diff --git a/README.md b/README.md index 442fea1..9af96a6 100644 --- a/README.md +++ b/README.md @@ -3,16 +3,16 @@ Set of bash scripts for analysis of HybSeq raw data. Consists of several steps: -0. Download FASTQ files from Illumina BaseSpace storage -1. Processing raw reads (PhiX removal, adaptor removal, quality filtering, summary statistics) -2. Mapping reads to reference (using Bowtie), create consensus sequence -3. Recognize sequences matching probes (generate PSLX files using BLAT) -4. Create alignments for all genes -5. Treat missing data, select best genes -6. Generate FastTree or RAxML gene trees + trees-alignment properties -7. Root gene trees with outgroup, combine gene trees into a single file -8. Estimate species tree (ASTRAL, ASTRID, MRL, concatenation) -9. Subselect suitable genes and repeat steps 6+7 +0: Download FASTQ files from Illumina BaseSpace storage +1: Processing raw reads (PhiX removal, adaptor removal, quality filtering, summary statistics) +2: Mapping reads to reference (using Bowtie), create consensus sequence +3: Recognize sequences matching probes (generate PSLX files using BLAT) +4: Create alignments for all genes +5: Treat missing data, select best genes +6: Generate FastTree or RAxML gene trees + trees-alignment properties +7: Root gene trees with outgroup, combine gene trees into a single file +8: Estimate species tree (ASTRAL, ASTRID, MRL, concatenation) +9: Subselect suitable genes and repeat steps 7+8 Uses many additional software that must be installed and put in the PATH prior to run scripts (see Table located in docs folder and consider to run install_software.sh). Also utilizes many scripts developed by others (located in HybSeqSource folder). PLEASE CITE APPROPRIATELY THOSE SCRIPTS WHEN USING HybPhyloMaker!