diff --git a/README.md b/README.md index 27f8973..c07ec60 100644 --- a/README.md +++ b/README.md @@ -9,7 +9,7 @@ 1: Processing raw reads (PhiX removal, adaptor removal, quality filtering, summary statistics) 2: Mapping reads to reference (using Bowtie2/BWA), create consensus sequence 3: Recognize sequences matching probes (generate PSLX files using BLAT) -4: Create alignments for all genes (+ correct reading frame) +4: Create alignments for all genes (+ optionally correct reading frame and/or select low heterozygosity loci) 5: Treat missing data, select best genes 6: Generate FastTree or RAxML gene trees + calculate/plot trees-alignment properties 7: Root gene trees with outgroup, combine gene trees into a single file