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Hello!
I'm trying to use scArches to do a label transfer between a reference and a query, and I have a few questions:
Is this normalization enough, or should it be done in a specific way?
sc.pp.normalize_total(adata, target_sum=1e4)
# Logarithmize the data:
sc.pp.log1p(adata)
Then, I want to use scPoli, but the model is asking about the condition_key. Is it enough to create a dummy variable where the condition for all the labels in the reference is the same? (All my cells come from the same condition-batch). Like: adata.obs['condition'] = 'none'
What does cell_type_key refer to? Should I specify the cell-type column that I want to use to transfer the labels here?
I see that you recommend scANVI also for annotation. Could any other method in scArches (like scGen) be useful for this task?
Thanks!
The text was updated successfully, but these errors were encountered:
Hello!
I'm trying to use scArches to do a label transfer between a reference and a query, and I have a few questions:
Is this normalization enough, or should it be done in a specific way?
Then, I want to use scPoli, but the model is asking about the condition_key. Is it enough to create a dummy variable where the condition for all the labels in the reference is the same? (All my cells come from the same condition-batch). Like:
adata.obs['condition'] = 'none'
What does cell_type_key refer to? Should I specify the cell-type column that I want to use to transfer the labels here?
I see that you recommend scANVI also for annotation. Could any other method in scArches (like scGen) be useful for this task?
Thanks!
The text was updated successfully, but these errors were encountered: