All notable changes to this project will be documented in this file.
The format is based on Keep a Changelog, and this project adheres to Semantic Versioning.
- new parameters for genome QC:
seq_threshold
andn_threshold
- the default values did not change
- updated default
nextclade
version to3.3.1
- default dataset version
2024-02-16--04-00-32Z
- default dataset version
- updated default
pangolin
version to4.3
- default
pangolin-data
version 1.25.1
- default
- fixed conda channel order
- enhanced nextclade dataset provenance
- added a DOI
- update documentation
- new parameters
nextclade_dataset_name
andnextclade_dataset_tag
for betternextclade
fine tuning- with
--update
the latest dataset tag is selected
- with
- new filter for indels for consensus generation based on their frequency
--cns_indel_filter
- fixed conda/mamba execution in standard profile
- updated smallest required
nextflow
version to22.10.0
- updated default
nextclade
version to 2.13.1 - updated default
pangolin
version to 4.2 withpangolin-data
version 1.18.1.1
- corrected column name in summary report table
- timestamp in report name
- updated conda config
- added license (GPL3)
sc2rf
resources from https://github.com/ktmeaton/ncov-recombinant instead of https://github.com/lenaschimmel/sc2rf
- test profile
- end-to-end CI test
- Report (accumulated sequence depth plot) can handle > 90 input samples
- default reference changed from none to 'sars-cov-2'
- full support for single end data
- '--bamclipper_additional_parameters' option to adjust BAMClipper parameters
- documentation on how names in the input BED file have to be to automatically generate a BEDPE file
- added
pangolin-data
version to th epangolin environment/container - added an insert size filter for the BAM file
- RKI report bug
- fixed Nextclade2 Docker auto-update
- updated Nextclade to version 2: CoVpipe2 not compatible with nextclade version
< 2.4.0
- updated Pangolin to version 4.1.2
- parameter
pangolin_scorpio
, since pangolin default behaviour changed from v4.1
- report can handle Kraken2 output with no human, unclassified or tax_id reads
- changed
bcftools consensus
parameter from-I
to-H I
- does not change behaviour
- genotype adjustment for all heterzygote variants
- updated default pangolin version to 4.0.6
- renamed parameter
pangolin_skip_scorpio
topangolin_scorpio
(default behavior of skipping scorpio does not change) - Kraken2 read output is not compressed - changed the file naming accordingly
- removed unsued script
- removed unused conda yamls
- added sc2rf
- strict params to find potential recombinants
- added skip-scorpio parameter for pangolin
- activeted per defaul
- added this changelog
- separate result tabled get published
- better primer/reference id check
- fist fasta id has to be at least once in the primer file (some primer kits have a non-sc2 amplicon)
- kraken2 database can be set by user
- hard filtered variants get published in the variant calling result directory
- removed
?
removal since deletion adjustment is removed - update processes for pangolin and nextclade
- fixed container execution for pangolin and nextclade
- fixed disabling/enabling of
var_sap
parameter
- updated workflow figure
- more stable automated conda update for pangolin and nextclade directly from anaconda.org
- updated help/readme
- removed deletion adjustment since bcftools works as expected
- LCS update functionality
- LCS fro mixed sample detection
- updated versions of samtools, liftoff, kraken, freebayes, bcftools, fastp, bedtools
bedtools subtract
instead ofintersect
for low coverage mask generation
- updated help and readme
- disabel
var-sap
in default - removed workflow version from consensus fasta header
- fixed krona fails with mamba/conda execution
- fixes pangolin update
- update nextclade
- DESH support
- initial commit