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Dockerfile
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# start with miniconda3 as build environment
FROM continuumio/miniconda3 AS build
# Update, install mamba and conda-pack:
RUN conda update -n base -c defaults --yes conda && \
conda install -c conda-forge -n base --yes mamba conda-pack
# Install funannotate deps from bioconda
# here specifying specific versions to be able to set ENV below
RUN mamba create -c conda-forge -c bioconda -c defaults \
-n funannotate --yes "python>=3.6,<3.9" biopython \
"trinity==2.8.5" "evidencemodeler==1.1.1" "pasa==2.4.1" "codingquarry==2.0" \
"proteinortho==6.0.16" goatools matplotlib-base natsort numpy pigz \
pandas psutil requests scikit-learn scipy seaborn "blast=2.2.31" \
tantan bedtools hmmer exonerate "diamond>=2.0.5" tbl2asn blat "trnascan-se>=2.0" \
ucsc-pslcdnafilter trimmomatic raxml iqtree trimal "mafft>=7" hisat2 \
"kallisto==0.46.1" minimap2 stringtie "salmon>=0.9" "samtools>=1.9" \
glimmerhmm bamtools perl perl-local-lib perl-dbd-mysql perl-clone perl-hash-merge \
perl-soap-lite perl-json perl-logger-simple perl-scalar-util-numeric perl-math-utils perl-mce \
perl-text-soundex perl-parallel-forkmanager perl-db-file perl-perl4-corelibs ete3 distro \
&& conda clean -a -y
# Since we want the most recent, install from repo, remove snap as broken
SHELL ["conda", "run", "-n", "funannotate", "/bin/bash", "-c"]
RUN python -m pip install git+https://github.com/nextgenusfs/funannotate.git
# package with conda-pack
RUN conda-pack -n funannotate -o /tmp/env.tar && \
mkdir /venv && cd /venv && tar xf /tmp/env.tar && \
rm /tmp/env.tar
# We've put venv in same path it'll be in final image
RUN /venv/bin/conda-unpack
# Now build environment
FROM debian:buster AS runtime
# Copy /venv from the previous stage:
COPY --from=build /venv /venv
# Install debian snap via apt-get
RUN apt-get update && apt-get install -y snap augustus augustus-data locales locales-all libgl1 && \
rm -rf /var/lib/apt/lists/* && \
ln -s /usr/bin/snap-hmm /usr/bin/snap && \
rm "/venv/bin/fasta" && \
ln -s "/venv/bin/fasta36" "/venv/bin/fasta"
# add it to the PATH and add env variables
ENV PATH="/venv/bin:$PATH" \
AUGUSTUS_CONFIG_PATH="/usr/share/augustus/config" \
EVM_HOME="/venv/opt/evidencemodeler-1.1.1" \
PASAHOME="/venv/opt/pasa-2.4.1" \
TRINITYHOME="/venv/opt/trinity-2.8.5" \
QUARRY_PATH="/venv/opt/codingquarry-2.0/QuarryFiles" \
ZOE="/usr/share/snap" \
USER="me" \
FUNANNOTATE_DB="/opt/databases"
# When image is run, run the code with the environment
SHELL ["/bin/bash", "-c"]
CMD funannotate