diff --git a/tests/api/test_flexible_calling.py b/tests/api/test_flexible_calling.py
index 74bf8f4d..7b7b1499 100644
--- a/tests/api/test_flexible_calling.py
+++ b/tests/api/test_flexible_calling.py
@@ -48,26 +48,6 @@ def test_invalid_str(self):
                 "ERROR:root:working directory specified is not a valid string.",
             )
 
-    def test_invalid_escape_sequence_path(self):
-        """This test checks when working directory is not a invalid escape sequence string,
-        if the program will behave correctly"""
-        with self.assertLogs() as logobs:
-            json_case_path = "./tests/api/data/flexible_calling_unit_test/verification_case_unit_test_Path.json"
-
-            # Change working_dir value in the json file to a invalid string
-            with open(json_case_path, "r") as f:
-                workflow_dict = json.load(f)
-                workflow_dict["working_dir"] = ".\tests\api\result"
-
-            with open(json_case_path, "w") as f:
-                json.dump(workflow_dict, f)
-
-            workflow = Workflow(workflow=json_case_path)
-            self.assertEqual(
-                logobs.output[0],
-                "ERROR:root:The working directory specified is an invalid escape sequence string.",
-            )
-
     def test_Linux_path(self):
         """This test check if the program can detect the working path provided is in Linux format."""
         with self.assertLogs() as logobs: