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Basic Melting Temperature #1

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AHReccese opened this issue May 4, 2024 · 2 comments · Fixed by #27
Closed

Basic Melting Temperature #1

AHReccese opened this issue May 4, 2024 · 2 comments · Fixed by #27
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@AHReccese
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AHReccese commented May 4, 2024

Description

  • Basic

http://biotools.nubic.northwestern.edu/OligoCalc.html

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Expected Behavior

Actual Behavior

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Python Version

OPR Version (Use : opr.__version__)

@AHReccese AHReccese self-assigned this Jun 25, 2024
@AHReccese AHReccese added this to the opr v0.1 milestone Jun 25, 2024
@sepandhaghighi sepandhaghighi changed the title Melting Temperature Basic Melting Temperature Sep 29, 2024
@sepandhaghighi
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Reference:

  1. Marmur, J. and Doty, P. 1962 Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol.5109–118

  2. Alejandro Panjkovich, Francisco Melo, Comparison of different melting temperature calculation methods for short DNA sequences, Bioinformatics, Volume 21, Issue 6, March 2005, Pages 711–722, https://doi.org/10.1093/bioinformatics/bti066

Formula:

$${T}_{m}=64.9+41.0\times \left(\frac{y\hbox{ G }+z\hbox{ C }-16.4}{w\hbox{ A }+x\hbox{ T }+y\hbox{ G }+z\hbox{ C }}\right)$$

@sepandhaghighi
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@AHReccese
Feel free to begin this task.

@sepandhaghighi sepandhaghighi linked a pull request Nov 24, 2024 that will close this issue
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