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Ivy edited this page Aug 26, 2021 · 56 revisions

ecc_finder Version: v1.0.0

ecc_finder identifies eccDNA loci using short and long reads.

Long read mapping

To identify eccDNA loci by mapping to a reference genome.

Algorithm overview

Long read pipeline algorithm overview

Usage

Usage in details, please see the map-ont

usage: ecc_finder.py map-ont <reference.idx> <query.fq> (option)

Short read mapping

Algorithm overview

Long read pipeline algorithm overview

Usage

usage: ecc_finder.py map-sr <reference.idx> <query.fq> (option)

Long read assembly

Long read pipeline algorithm overview

Usage

usage: ecc_finder.py asm-ont <query.fq> (option)

Short read assembly

Long read pipeline algorithm overview

Usage

usage: ecc_finder.py asm-sr <query.fq> (option)

Output

All output is in eccFinder_output, or whichever directory -o specifies.

eccDNA.fasta

The eccDNA locus in FASTA format.

eccDNA.csv

The eccDNA locus in csv format.

Col Type Description
1 string Reference sequence name
2 int Reference start on original strand
3 int Reference start on original strand
4 int Circular read number at the locus
5 int Repeat units of all circular reads
6 int Read coverage at the locus
7 int EccDNA sequence length
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