You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
Pool the reads from both experiments into a single Tag Directory using makeTagDirectory (more details here). In the case of technical replicates (i.e. runs from the same sequencing library), it is advisable to always "pool" the data. If they are biological replicates, it is often a good idea to keep them separate for and take advantage of their variability to refine your analysis. For some types of analysis, such as transcript identification, it is a good idea to create a single META-experiment that contains all of the GRO-Seq reads for a given cell type. This will provide increased power for identifying transcripts de novo.
Description of feature
http://homer.ucsd.edu/homer/ngs/groseq/groseq.html
Currently, supporting technical reads getting pooled, and grouping via META-experiment.
I think either way, it requires some really careful samplesheet curation, and there aren't examples of what those would be exactly.
The text was updated successfully, but these errors were encountered: