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+### Tracy and Mario's Codes of Subsampling Stuff
+### Created 22 Aug 2012
+### Last Update 0 Oct 2012 - Muscarella
+
+# system( ) #mothur command version
+
+# Example files used for testing
+# input_file <- "KBS.an.shared" 
+
+# Needed packages
+require(vegan)
+
+# Read OTU File
+read.otu <- function(shared = " ", cutoff = "0.03"){
+  matrix <- read.delim(shared, head=T)
+  matrix.cutoff <- subset(matrix, matrix$label == cutoff)
+  matrix.out <- as.matrix(matrix.cutoff[1:dim(matrix.cutoff)[1],4:(3+mean(matrix.cutoff$numOtus))])
+  row.names(matrix.out) <- matrix.cutoff$Group
+  return(matrix.out)
+  } 
+
+# Count All Groups 
+count.groups <- function(otu.matrix = " "){
+  counts <- rowSums(otu.matrix)
+  return(counts)
+  }  
+
+# Subsampling wrapper
+sub.sample <- function(otu.matrix = " ", sample.size = "min(count.groups(test))"){
+  counts <- count.groups(otu.matrix) 
+  statement <- counts > sample.size  # Add warning message
+  otu.matrix <- subset(otu.matrix, rowSums(otu.matrix)>sample.size)
+  x <- rrarefy(otu.matrix, sample.size)
+  return(x)
+  }
+
+# Example function for calculating species richness  
+richness.iter <- function(shared = " ", cutoff = " ", size = " ", iters = " "){
+  otu.matrix <- read.otu(shared, cutoff)
+  counts <- count.groups(otu.matrix) 
+  statement <- counts > size  # Add warning message
+  otu.matrix <- subset(otu.matrix, rowSums(otu.matrix)>size)
+  rich.matrix <- matrix(NA, dim(otu.matrix)[1], iters)
+  rownames(rich.matrix) <- rownames(otu.matrix)
+  for (i in 1:iters){
+    temp.matrix <- sub.sample(otu.matrix, size)
+    rich.matrix[,i] <- rowSums((temp.matrix>0)*1)
+    }
+  return(rich.matrix)
+  } 
+  
+# Example function for calculating diversity
+diversity.iter <- function(shared = " ", index = "shannon", cutoff = " ", size = " ", iters = " "){
+  otu.matrix <- read.otu(shared, cutoff)
+  counts <- count.groups(otu.matrix) 
+  statement <- counts > size  # Add warning message
+  otu.matrix <- subset(otu.matrix, rowSums(otu.matrix)>size)
+  div.matrix <- matrix(NA, dim(otu.matrix)[1], iters)
+  rownames(div.matrix) <- rownames(otu.matrix)
+  for (i in 1:iters){
+    temp.matrix <- sub.sample(otu.matrix, size)
+    div.matrix[,i] <- diversity(temp.matrix, index)  
+    }
+  return(div.matrix)
+  }
+  
+# Example function for calculating species evenness (Pielou's Evenness = J' = H'/H'max = H'/lnS, H' = Shannon)
+evenness.iter <- function(shared = " ", cutoff = " ", size = " ", iters = " "){
+  otu.matrix <- read.otu(shared, cutoff)
+  counts <- count.groups(otu.matrix)
+  statement <- counts > size  # Add warning message
+  otu.matrix <- subset(otu.matrix, rowSums(otu.matrix)>size)
+  even.matrix <- matrix(NA, dim(otu.matrix)[1], iters)
+  rownames(even.matrix) <- rownames(otu.matrix)
+  for (i in 1:iters){
+    temp.matrix <- sub.sample(otu.matrix, size)
+    even.matrix[,i] <- diversity(temp.matrix, "shannon")/log(rowSums((temp.matrix>0)*1))
+    }
+  return(even.matrix)
+  } 
+