diff --git a/js/web/lib/wasm/jsep/backend-webgpu.ts b/js/web/lib/wasm/jsep/backend-webgpu.ts
index 78147ffc09ab7..39f8c2a6d0db3 100644
--- a/js/web/lib/wasm/jsep/backend-webgpu.ts
+++ b/js/web/lib/wasm/jsep/backend-webgpu.ts
@@ -593,7 +593,6 @@ export class WebGpuBackend {
         } else if (v.type === DataType.uint32) {
           new Uint32Array(arrayBuffer, offset, data.length).set(data);
         } else if (v.type === DataType.float16) {
-          // TODO: use Float16Array.
           new Uint16Array(arrayBuffer, offset, data.length).set(data);
         } else if (v.type === DataType.float) {
           new Float32Array(arrayBuffer, offset, data.length).set(data);
diff --git a/js/web/lib/wasm/jsep/init.ts b/js/web/lib/wasm/jsep/init.ts
index 3f326881079f0..b31fbc6255c41 100644
--- a/js/web/lib/wasm/jsep/init.ts
+++ b/js/web/lib/wasm/jsep/init.ts
@@ -3,8 +3,9 @@
 
 import { Env } from 'onnxruntime-common';
 
+import { calculateTensorSizeInBytes, DataType } from '../wasm-common';
+
 import type { OrtWasmModule } from '../wasm-types';
-import { DataType, calculateTensorSizeInBytes } from '../wasm-common';
 
 import { WebGpuBackend } from './backend-webgpu';
 import { LOG_DEBUG } from './log';
@@ -22,6 +23,14 @@ class TensorViewImpl implements TensorView {
     public readonly dims: readonly number[],
   ) {}
 
+  getUint16Array(): Uint16Array {
+    if (this.dataType !== DataType.float16 && this.dataType !== DataType.uint16) {
+      throw new Error('Invalid data type');
+    }
+    const elementCount = ShapeUtil.size(this.dims);
+    return elementCount === 0 ? new Uint16Array() : new Uint16Array(this.module.HEAP8.buffer, this.data, elementCount);
+  }
+
   getFloat32Array(): Float32Array {
     if (this.dataType !== DataType.float) {
       throw new Error('Invalid data type');
diff --git a/js/web/lib/wasm/jsep/tensor-view.ts b/js/web/lib/wasm/jsep/tensor-view.ts
index defc418c29264..5f1fdfa4534cd 100644
--- a/js/web/lib/wasm/jsep/tensor-view.ts
+++ b/js/web/lib/wasm/jsep/tensor-view.ts
@@ -28,6 +28,11 @@ export interface TensorView {
   readonly dataType: number;
   readonly dims: readonly number[];
 
+  /**
+   * get a Float16Array data view of the tensor data. tensor data must be on CPU.
+   */
+  getUint16Array(): Uint16Array;
+
   /**
    * get a Float32Array data view of the tensor data. tensor data must be on CPU.
    */
diff --git a/js/web/lib/wasm/jsep/webgpu/ops/pad.ts b/js/web/lib/wasm/jsep/webgpu/ops/pad.ts
index 4951bd0192baf..eb08cc7b713f2 100644
--- a/js/web/lib/wasm/jsep/webgpu/ops/pad.ts
+++ b/js/web/lib/wasm/jsep/webgpu/ops/pad.ts
@@ -165,8 +165,10 @@ const createPadProgramInfo = (inputs: readonly TensorView[], attributes: PadAttr
     { type: DataType.uint32, data: outputSize },
     { type: DataType.int32, data: attributes.pads },
   ];
+
+  const isValueFromInput = inputs.length >= 3 && inputs[2].data;
   if (attributes.mode === 0) {
-    programUniforms.push({ type: inputs[0].dataType, data: attributes.value });
+    programUniforms.push({ type: isValueFromInput ? inputs[2].dataType : DataType.float, data: attributes.value });
   }
 
   programUniforms.push(...createTensorShapeVariables(inputs[0].dims, outputShape));
@@ -182,7 +184,7 @@ const createPadProgramInfo = (inputs: readonly TensorView[], attributes: PadAttr
       { name: 'pads', type: 'i32', length: attributes.pads.length },
     ];
     if (attributes.mode === 0) {
-      uniforms.push({ name: 'constant_value', type: dataType as UniformDataElementType });
+      uniforms.push({ name: 'constant_value', type: (isValueFromInput ? dataType : 'f32') as UniformDataElementType });
     }
 
     return `
@@ -200,7 +202,7 @@ const createPadProgramInfo = (inputs: readonly TensorView[], attributes: PadAttr
 
   return {
     name: 'Pad',
-    shaderCache: { hint: `${attributes.mode}`, inputDependencies },
+    shaderCache: { hint: `${attributes.mode}${isValueFromInput}`, inputDependencies },
     getRunData: () => ({
       outputs: [{ dims: outputShape, dataType: inputs[0].dataType }],
       dispatchGroup: { x: Math.ceil(ShapeUtil.size(outputShape) / 64 /* workgroup size */) },
@@ -213,7 +215,12 @@ const createPadProgramInfo = (inputs: readonly TensorView[], attributes: PadAttr
 const createPadAttributesFromInputs = (inputs: readonly TensorView[], attributes: PadAttributes): PadAttributes => {
   if (inputs.length > 1) {
     const bigInt64Pads = inputs[1].getBigInt64Array();
-    const value = inputs.length >= 3 && inputs[2].data ? inputs[2].getFloat32Array()[0] : 0.0;
+    const value =
+      inputs.length >= 3 && inputs[2].data
+        ? inputs[2].dataType === DataType.float16
+          ? inputs[2].getUint16Array()[0]
+          : inputs[2].getFloat32Array()[0]
+        : 0.0;
 
     const inputRank = inputs[0].dims.length;
     const updatePads = new Int32Array(2 * inputRank).fill(0);
diff --git a/js/web/test/data/ops/pad_f16.jsonc b/js/web/test/data/ops/pad_f16.jsonc
index 44c61b8a95382..9e497cd2215ca 100644
--- a/js/web/test/data/ops/pad_f16.jsonc
+++ b/js/web/test/data/ops/pad_f16.jsonc
@@ -1,6 +1,6 @@
 [
   {
-    "name": "constant 2D float16",
+    "name": "constant 2D float16 v10",
     "operator": "Pad",
     "opset": { "domain": "", "version": 10 },
     "attributes": [
@@ -33,7 +33,7 @@
     ]
   },
   {
-    "name": "constant 2D float16",
+    "name": "constant 2D float16 v19",
     "operator": "Pad",
     "opset": { "domain": "", "version": 19 },
     "attributes": [{ "name": "mode", "data": "constant", "type": "string" }],
diff --git a/js/web/test/suite-test-list.jsonc b/js/web/test/suite-test-list.jsonc
index edbaeb6f4095c..829e55a625102 100644
--- a/js/web/test/suite-test-list.jsonc
+++ b/js/web/test/suite-test-list.jsonc
@@ -1385,6 +1385,7 @@
       "reduce-min.jsonc",
       "relu.jsonc",
       "gelu.jsonc",
+      "pad_f16.jsonc",
       "pad.jsonc",
       "pad-big.jsonc",
       "pow.jsonc",