diff --git a/onnxruntime/python/onnxruntime_inference_collection.py b/onnxruntime/python/onnxruntime_inference_collection.py
index 1a3e22142f80e..09f768f53ea65 100644
--- a/onnxruntime/python/onnxruntime_inference_collection.py
+++ b/onnxruntime/python/onnxruntime_inference_collection.py
@@ -466,7 +466,7 @@ def _create_inference_session(self, providers, provider_options, disabled_optimi
 
         session_options = self._sess_options if self._sess_options else C.get_default_session_options()
 
-        self._register_ep_custom_ops(session_options, providers, provider_options)
+        self._register_ep_custom_ops(session_options, providers, provider_options, available_providers)
 
         if self._model_path:
             sess = C.InferenceSession(session_options, self._model_path, True, self._read_config_from_model)
@@ -510,11 +510,15 @@ def _reset_session(self, providers, provider_options):
         self._sess_options = self._sess_options_initial
         self._create_inference_session(providers, provider_options)
 
-    def _register_ep_custom_ops(self, session_options, providers, provider_options):
+    def _register_ep_custom_ops(self, session_options, providers, provider_options, available_providers):
         for i in range(len(providers)):
-            if providers[i] == "TensorrtExecutionProvider":
+            if providers[i] in available_providers and providers[i] == "TensorrtExecutionProvider":
                 C.register_tensorrt_plugins_as_custom_ops(session_options, provider_options[i])
-            elif isinstance(providers[i], tuple) and providers[i][0] == "TensorrtExecutionProvider":
+            elif (
+                isinstance(providers[i], tuple)
+                and providers[i][0] in available_providers
+                and providers[i][0] == "TensorrtExecutionProvider"
+            ):
                 C.register_tensorrt_plugins_as_custom_ops(session_options, providers[i][1])