diff --git a/onnxruntime/test/python/onnxruntime_test_python.py b/onnxruntime/test/python/onnxruntime_test_python.py
index 2ecd12896dd58..91b6c71e735a8 100644
--- a/onnxruntime/test/python/onnxruntime_test_python.py
+++ b/onnxruntime/test/python/onnxruntime_test_python.py
@@ -414,8 +414,6 @@ def test_get_and_set_option_with_values(option_name, option_values):
                             str(option_value),
                         )
 
-                test_get_and_set_option_with_values("prefer_nhwc", ["1", "0"])
-
                 test_get_and_set_option_with_values("enable_cuda_graph", ["1", "0"])
 
                 test_get_and_set_option_with_values("arena_extend_strategy", ["kNextPowerOfTwo", "kSameAsRequested"])