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despite there's a recombinant sequence (in case of HCV just one, HCV-1b/2k) which is very close to the actual sequence (created with SmaltAlign), MinVar chose a non recombinant sequence as reference:
in minvar.logINFO 2018/11/29 09:13:23 prepare.py: main() 472: Using non recombinant
This then leads to a cns_ambiguous.fasta sequence which contains a lot of Ns
The recombinant form is also not listed in subtype_evidence.csv
The text was updated successfully, but these errors were encountered:
The logic in MinVar is that if the viral reads are aligned to a non_recombinant reference more than 50% then do not try the recombinant reference sequences.
Example
1000421130
despite there's a recombinant sequence (in case of HCV just one, HCV-1b/2k) which is very close to the actual sequence (created with SmaltAlign), MinVar chose a non recombinant sequence as reference:
in
minvar.log
INFO 2018/11/29 09:13:23 prepare.py: main() 472: Using non recombinant
This then leads to a
cns_ambiguous.fasta
sequence which contains a lot ofN
sThe recombinant form is also not listed in
subtype_evidence.csv
The text was updated successfully, but these errors were encountered: