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<!DOCTYPE html>
<html>
<head>
<meta charset="utf-8">
<title>Strobe-enhanced microscopy stage</title>
<meta http-equiv="X-UA-Compatible" content="IE=edge">
<meta name="viewport" content="width=device-width, initial-scale=1">
<link rel="shortcut icon" href="./assets/favicon.ico" />
<link href="./static/style.css" rel="stylesheet">
<link href="./assets/style.css" rel="stylesheet">
</head>
<body>
<header class="site-header">
<div class="wrapper header-wrapper" >
<div class=header-text>
<h1 class="site-title">Strobe-enhanced microscopy stage</h1>
<p class="author">by Pierre Padilla-Huamantinco, Matías Hurtado-Labarca, and Tobias Wenzel</p>
<p class="affiliation">Latin American Hub for Bioimaging Through Open Hardware (LIBRE hub)</p>
</div>
</div>
</header>
<div class="page-content">
<div>
<nav class="sidebar">
<a href="#" class="menu-icon">
<svg viewBox="0 0 18 15">
<path fill="#424242" d="M18,1.484c0,0.82-0.665,1.484-1.484,1.484H1.484C0.665,2.969,0,2.304,0,1.484l0,0C0,0.665,0.665,0,1.484,0 h15.031C17.335,0,18,0.665,18,1.484L18,1.484z"/>
<path fill="#424242" d="M18,7.516C18,8.335,17.335,9,16.516,9H1.484C0.665,9,0,8.335,0,7.516l0,0c0-0.82,0.665-1.484,1.484-1.484 h15.031C17.335,6.031,18,6.696,18,7.516L18,7.516z"/>
<path fill="#424242" d="M18,13.516C18,14.335,17.335,15,16.516,15H1.484C0.665,15,0,14.335,0,13.516l0,0 c0-0.82,0.665-1.484,1.484-1.484h15.031C17.335,12.031,18,12.696,18,13.516L18,13.516z"/>
</svg>
</a>
<ul class="nav-list">
<li>
<a class="navhome" href="./">
Strobe-enhanced microscopy stage
</a>
</li>
<li class="active">
<a class="active" href="./3-level-station.html">
Build the 3-level microscopy stage
</a>
<ul class="sub-nav-list">
<li>
<a class="active" href="./printing.html">
Print the plastic parts
</a>
</li>
<li>
<a class="not-active" href="./laser-cutting.html">
Laser cut the acrylic parts
</a>
</li>
<li>
<a class="not-active" href="./assemble-focus-mechanism.html">
Assemble the focus mechanism
</a>
</li>
<li>
<a class="not-active" href="./basic-optics-module.html">
Assemble the basics optics module
</a>
</li>
<li>
<a class="not-active" href="./attach-parts-to-top-plate.html">
Attach parts to the top plate
</a>
</li>
<li>
<a class="not-active" href="./attach-parts-to-middle-plate.html">
Attach parts to the middle plate
</a>
</li>
<li>
<a class="not-active" href="./attach-parts-to-bottom-plate.html">
Attach parts to the bottom plate
</a>
</li>
<li>
<a class="not-active" href="./mount-optics.html">
Mount the optics
</a>
</li>
<li>
<a class="not-active" href="./wiring.html">
Mount the electronics
</a>
</li>
<li>
<a class="not-active" href="./illumination.html">
Assemble the illumination
</a>
</li>
<li>
<a class="not-active" href="./finished.html">
Assembly complete
</a>
</li>
</ul>
</li>
<li class="not-active">
<a class="not-active" href="./software/software-installation.html">
Software Installation
</a>
</li>
<li class="not-active">
<a class="not-active" href="./usage.html">
Use your Stage
</a>
</li>
<li class="not-active">
<a class="not-active" href="./troubleshooting.html">
Troubleshooting
</a>
</li>
<li class="not-active">
<a class="not-active" href="./variants_accesories.html">
Customisation and accessories
</a>
</li>
</ul>
</nav>
</div>
<div class="wrapper">
<h1 id="print-the-plastic-parts">Print the plastic parts</h1>
<div class="pagebom">
<h3 id="tool">Tools</h3>
<ul>
<li>1 <a Class="bom" href="parts/tools/precision-wire-cutters.html">Precision wire cutter</a> </li>
<li>1 <a Class="bom" href="parts/tools/rep-rap.html">RepRap-style printer</a> </li>
<li>1 <a Class="bom" href="parts/tools/utility-knife.html">Utility knife</a> - Not a scalpel!</li>
</ul>
<h3 id="material">Materials</h3>
<ul>
<li>50 g of <a Class="bom" href="parts/materials/black_pla_filament.html">Black PLA filament</a> </li>
<li>205 g of <a Class="bom" href="parts/materials/pla_filament.html">PLA filament</a> - Of any color you want</li>
</ul>
</div>
<h2 class="page-step" id="set-your-printer-settings">Step 1: Set your printer settings</h2>
<p>Almost all station parts can be printed out of <a href="parts/materials/pla_filament.html">PLA filament</a> on most <a href="parts/tools/rep-rap.html">RepRap-style printers</a>.</p>
<p>We recommend the following printer settings:</p>
<table>
<thead>
<tr>
<th>Setting</th>
<th>Value</th>
</tr>
</thead>
<tbody>
<tr>
<td>Material</td>
<td>PLA</td>
</tr>
<tr>
<td>Material Temperature</td>
<td>Recommended by the PLA brand</td>
</tr>
<tr>
<td>Layer height</td>
<td>0.2mm or less</td>
</tr>
<tr>
<td>Infill</td>
<td>Printer default or more</td>
</tr>
<tr>
<td>Brim</td>
<td>Recommended for all parts</td>
</tr>
<tr>
<td>Slice gap closing radius</td>
<td>0.001mm</td>
</tr>
</tbody>
</table>
<p>Test whether your printer can print the pieces for this station or other open-source designs. Download and print the <a href="models/leg_test.html">leg test</a> file. This will only use about <a href="parts/materials/pla_filament.html">5 grams of PLA</a>.</p>
<p>The result should look like this (this has been printed with a brim):</p>
<p><img alt="" src="images/just_leg_test.jpg" title="" /></p>
<p>As a general rule, strength is more important than surface finish, so very thin layers (less than 0.15mm or so) are unlikely to result in a station that performs any better, though it may improve the appearance.</p>
<h2 class="page-step" id="download-and-printing">Step 2: Download and Printing</h2>
<p>Now that you have tested your <a href="parts/tools/rep-rap.html">3D printer</a> and <a href="parts/materials/pla_filament.html">filament</a> you can print the plastic parts:</p>
<p>You can have access to all the STLs in <a href="https://www.printables.com/model/647564" target="_blank" title=""><strong>Printables</strong></a> or go for free to our <a href="https://cad.onshape.com/documents/56f3731ad1d54872e3d730b5/w/79a6f9b65ec1b22fe0bc7f1a/e/d497faac00a6e40fe8fec4e7" target="_blank" title=""><strong>Online CAD software</strong></a> to make your own version. You can also download them individually here:</p>
<ul>
<li>Actuator assembly tools (<a name="output__nut-tool"></a>nut tool, <a name="output__band-tool"></a>band tool, and <a name="output__band-tool-cover"></a>band tool cover): <a href="models/actuator-assembly-tools.html" title="">actuator_assembly_tools.stl</a></li>
<li><a name="output__focusing-assembly"></a>Focusing assembly: <a href="models/focusing-assembly.html" title="">focusing-assembly.stl</a></li>
<li><a name="output__actuator-foot"></a>Actuator Foot: <a href="models/z_foot.html" title="">z_foot.stl</a></li>
<li><a name="output__thumbwheel"></a>Thumbwheel: <a href="models/thumbwheel.html" title="">thumbwheel.stl</a></li>
<li><a name="output__pi-camera-platform"></a>Pi camera platform: <a href="models/camera-platform.html" title="">camera-platform.stl</a></li>
<li><a name="output__lens-spacer"></a>Lens spacer: <a href="models/lens-spacer.html" title="">lens-spacer.stl</a> - <strong>This must be printed in <a href="parts/materials/black_pla_filament.html">black</a>!</strong></li>
<li><a name="output__illumination-base"></a>Illumination base: <a href="models/illumination-base.html" title="">illumination-base.stl</a> - <strong>This must be printed with supports</strong></li>
<li><a name="output__illumination-slider"></a>Illumination slider: <a href="models/illumination-slider.html" title="">illumination-slider.stl</a></li>
<li><a name="output__illumination-thumbscrew"></a>Illumination thumbscrew: <a href="models/illumination-thumbscrew.html" title="">illumination-thumbscrew.stl</a></li>
<li><a name="output__illumination-arm"></a>Illumination arm: <a href="models/led-arm.html" title="">led-arm.stl</a> - <strong>This must be printed with supports</strong></li>
<li><a name="output__hinge-pin"></a>Hinge pin: <a href="models/arm-pin.html" title="">arm-pin.stl</a></li>
<li><a name="output__led-cover"></a>LED cover: <a href="models/led-cover.html" title="">led-cover.stl</a></li>
<li><a name="output__standoff-s"></a>Standoff-S: <a href="models/standoff-S.html" title="">standoff-S.stl</a></li>
<li><a name="output__standoff-m"></a>Standoff-M: <a href="models/standoff-M.html" title="">standoff-M.stl</a></li>
<li><a name="output__foot-m"></a>Foot-M: <a href="models/foot-M.html" title="">foot-M.stl</a></li>
<li><a name="output__spacer-s"></a>Spacer-S: <a href="models/spacer-4mm.html" title="">spacer-4mm.stl</a></li>
<li><a name="output__spacer-m"></a>Spacer-M: <a href="models/spacer-11mm.html" title="">spacer-11mm.stl</a></li>
</ul>
<h2 class="page-step" id="clean-up-of-printed-parts">Step 3: Clean-up of printed parts</h2>
<div class="warning-block">
<p><strong>Be careful when removing the brim:</strong> To avoid injury, first remove the bulk of the brim without a knife. Remove the remaining brim with a peeling action as described below.</p>
</div>
<p>Carefully remove the printing brim from all parts. To remove brim:</p>
<ol>
<li>Use <a href="parts/tools/precision-wire-cutters.html">precision wire cutters</a> to remove most of the brim from the part.</li>
<li>Clean up the remaining brim with a <a href="parts/tools/utility-knife.html">utility knife</a>:<ul>
<li>Hold the knife in your dominant hand with 4 fingers curled around the handle, leaving your thumb free.</li>
<li>Hold the part in your other hand, as far away from the surface, to be cut as possible.</li>
<li>Support the part with the thumb of your dominant hand.</li>
<li>Place the blade on the surface to be cut, and carefully close your dominant hand, moving the blade, under control, towards your thumb.</li>
</ul>
</li>
</ol>
<p><img alt="" src="images/BrimRemoval.png" title="" /></p>
<hr />
<p><a href="3-level-station_BOM.html">Previous page</a> | <a href="laser-cutting.html">Next page</a></p>
</div>
</div>
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<p class="author">©2024 Pierre Padilla-Huamantinco, Matías Hurtado-Labarca, and Tobias Wenzel</p>
<p class="email">Contact: <a href="mailto:[email protected]">[email protected]</a></p>
<p class="license">Strobe-enhanced microscopy stage is released under <a href="license.html">CERN-OHL-S-2.0</a>.</p>
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