diff --git a/e2e/README.md b/e2e/README.md
index 98fea127cb..9bd33df8a9 100644
--- a/e2e/README.md
+++ b/e2e/README.md
@@ -41,7 +41,16 @@ export LONGHORN_CLIENT_URL=http://node-public-ip:30000
 export LONGHORN_CLIENT_URL=http://localhost:8080
 ```
 
-5. Prepare test environment and run the test
+4. For running backup related test cases, export the related environment variable:
+
+(Currently only s3 backup target is supported)
+
+```
+export LONGHORN_BACKUPSTORE='s3://backupbucket@us-east-1/backupstore$minio-secret'
+export LONGHORN_BACKUPSTORE_POLL_INTERVAL=30
+```
+
+5. Prepare test environment and run the test:
 ```
 cd e2e
 python -m venv .