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mitogenome lost #44

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JWDebler opened this issue Mar 31, 2021 · 7 comments
Open

mitogenome lost #44

JWDebler opened this issue Mar 31, 2021 · 7 comments

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@JWDebler
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Hi,
I just ran raven over some of my nanopore data, and the assembly looks great, with one caveat, it lost the complete mitogenome. The same reads assembled by Canu lead to more fragmented contigs, but a tandem repeat of 3-4 mitogenomes. NECAT assembled the mitogenome into a single copy contig, but Raven seems to have discarded all the mitogenome reads as I cannot find any of the mito sequences anywhere in the assembly.
Any idea what happend or how to find out?
Cheers.
Raven.Cereal file

@rvaser
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rvaser commented Mar 31, 2021

Hi,
I'll check the cereal file, but please tell me the Raven version you are using.

Best regards,
Robert

@JWDebler
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@rvaser Sorry, was away over the extended easter break. That was with version 1.5.0

@rvaser
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rvaser commented Apr 16, 2021

What is the expected size of the mitochondrial DNA? I inspected the cereal file but not sure what to look for.

@JWDebler
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It's 52504 bp. Would the sequence help?

@rvaser
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rvaser commented Apr 18, 2021

It could help :)

@JWDebler
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Here you go: mitogenome.zip

@rvaser
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rvaser commented Jun 29, 2021

@JWDebler, I inspected the GFA in more detail, there is a contig consisting of two reads that has 2.5x the mitochondrial genome. Raven did not print it as it is not circular and has only two connected reads. Sorry for the huge delay.

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