diff --git a/lib/galaxy/tool_util/verify/asserts/image.py b/lib/galaxy/tool_util/verify/asserts/image.py
index ec5fb01d52da..f0fbc6e8326e 100644
--- a/lib/galaxy/tool_util/verify/asserts/image.py
+++ b/lib/galaxy/tool_util/verify/asserts/image.py
@@ -241,6 +241,7 @@ def cast_label(label):
 def assert_has_image_n_labels(
     output_bytes: bytes,
     channel: Optional[Union[int, str]] = None,
+    labels: Optional[Union[str, List[int]]] = None,
     exclude_labels: Optional[Union[str, List[int]]] = None,
     n: Optional[Union[int, str]] = None,
     delta: Union[int, str] = 0,
@@ -251,7 +252,7 @@ def assert_has_image_n_labels(
     """
     Asserts the specified output is an image and has the specified number of unique values (e.g., uniquely labeled objects).
     """
-    present_labels = _get_image_labels(output_bytes, channel, exclude_labels)[1]
+    present_labels = _get_image_labels(output_bytes, channel, labels, exclude_labels)[1]
     _assert_number(
         len(present_labels),
         n,
diff --git a/test/functional/tools/validation_image.xml b/test/functional/tools/validation_image.xml
index 852e78191a12..a1a7791fa6ec 100644
--- a/test/functional/tools/validation_image.xml
+++ b/test/functional/tools/validation_image.xml
@@ -102,6 +102,7 @@
                     <has_image_height height="32" />
                     <has_image_channels channels="1" />
                     <has_image_n_labels n="1" exclude_labels="0" />
+                    <has_image_n_labels n="0" exclude_labels="0,1" />
                     <has_image_mean_object_size mean_object_size="256" exclude_labels="0" />
                 </assert_contents>
             </output>
@@ -179,4 +180,4 @@
             </output>
         </test>
     </tests>
-</tool>
\ No newline at end of file
+</tool>