diff --git a/lib/galaxy/objectstore/cloud.py b/lib/galaxy/objectstore/cloud.py
index 2d72e49ad795..0a7f80e37b31 100644
--- a/lib/galaxy/objectstore/cloud.py
+++ b/lib/galaxy/objectstore/cloud.py
@@ -117,6 +117,8 @@ def _get_connection(provider, credentials):
         log.debug(f"Configuring `{provider}` Connection")
         if provider == "aws":
             config = {"aws_access_key": credentials["access_key"], "aws_secret_key": credentials["secret_key"]}
+            if "region" in credentials:
+                config["aws_region_name"] = credentials["region"]
             connection = CloudProviderFactory().create_provider(ProviderList.AWS, config)
         elif provider == "azure":
             config = {
@@ -184,8 +186,9 @@ def parse_xml(clazz, config_xml):
             if provider == "aws":
                 akey = auth_element.get("access_key")
                 skey = auth_element.get("secret_key")
-
                 config["auth"] = {"access_key": akey, "secret_key": skey}
+                if "region" in auth_element:
+                    config["auth"]["region"] = auth_element["region"]
             elif provider == "azure":
                 sid = auth_element.get("subscription_id")
                 if sid is None:
diff --git a/test/unit/objectstore/test_objectstore.py b/test/unit/objectstore/test_objectstore.py
index 897f1027b64f..57decbaa93dc 100644
--- a/test/unit/objectstore/test_objectstore.py
+++ b/test/unit/objectstore/test_objectstore.py
@@ -8,6 +8,7 @@
 from uuid import uuid4
 
 import pytest
+from requests import get
 
 from galaxy.exceptions import ObjectInvalid
 from galaxy.objectstore.azure_blob import AzureBlobObjectStore
@@ -1242,7 +1243,7 @@ def test_config_parse_azure_no_cache():
             assert object_store.staging_path == directory.global_config.object_store_cache_path
 
 
-def verify_caching_object_store_functionality(tmp_path, object_store):
+def verify_caching_object_store_functionality(tmp_path, object_store, check_get_url=True):
     # Test no dataset with id 1 exists.
     absent_dataset = MockDataset(1)
     assert not object_store.exists(absent_dataset)
@@ -1322,14 +1323,13 @@ def verify_caching_object_store_functionality(tmp_path, object_store):
 
     # Test get_object_url returns a read-only URL
     url = object_store.get_object_url(hello_world_dataset)
-    from requests import get
+    if check_get_url:
+      response = get(url)
+      response.raise_for_status()
+      assert response.text == "Hello World!"
 
-    response = get(url)
-    response.raise_for_status()
-    assert response.text == "Hello World!"
 
-
-def verify_object_store_functionality(tmp_path, object_store):
+def verify_object_store_functionality(tmp_path, object_store, check_get_url=True):
     # Test no dataset with id 1 exists.
     absent_dataset = MockDataset(1)
     assert not object_store.exists(absent_dataset)
@@ -1376,11 +1376,10 @@ def verify_object_store_functionality(tmp_path, object_store):
 
     # Test get_object_url returns a read-only URL
     url = object_store.get_object_url(hello_world_dataset)
-    from requests import get
-
-    response = get(url)
-    response.raise_for_status()
-    assert response.text == "Hello World!"
+    if check_get_url:
+        response = get(url)
+        response.raise_for_status()
+        assert response.text == "Hello World!"
 
 
 AZURE_BLOB_TEMPLATE_TEST_CONFIG_YAML = """
@@ -1509,6 +1508,108 @@ def test_real_azure_blob_store_in_hierarchical(tmp_path):
         verify_object_store_functionality(tmp_path, object_store)
 
 
+AMAZON_S3_SIMPLE_TEMPLATE_TEST_CONFIG_YAML = """
+type: aws_s3
+store_by: uuid
+auth:
+  access_key: ${access_key}
+  secret_key: ${secret_key}
+
+bucket:
+  name: ${bucket}
+
+extra_dirs:
+- type: job_work
+  path: database/job_working_directory_azure
+- type: temp
+  path: database/tmp_azure
+"""
+
+
+@skip_unless_environ("GALAXY_TEST_AWS_ACCESS_KEY")
+@skip_unless_environ("GALAXY_TEST_AWS_SECRET_KEY")
+@skip_unless_environ("GALAXY_TEST_AWS_BUCKET")
+def test_real_aws_s3_store(tmp_path):
+    template_vars = {
+        "access_key": os.environ["GALAXY_TEST_AWS_ACCESS_KEY"],
+        "secret_key": os.environ["GALAXY_TEST_AWS_SECRET_KEY"],
+        "bucket": os.environ["GALAXY_TEST_AWS_BUCKET"],
+    }
+    with TestConfig(AMAZON_S3_SIMPLE_TEMPLATE_TEST_CONFIG_YAML, template_vars=template_vars) as (_, object_store):
+        verify_caching_object_store_functionality(tmp_path, object_store)
+
+
+AMAZON_CLOUDBRIDGE_TEMPLATE_TEST_CONFIG_YAML = """
+type: cloud
+store_by: uuid
+provider: aws
+auth:
+  access_key: ${access_key}
+  secret_key: ${secret_key}
+
+bucket:
+  name: ${bucket}
+
+extra_dirs:
+- type: job_work
+  path: database/job_working_directory_azure
+- type: temp
+  path: database/tmp_azure
+"""
+
+
+@skip_unless_environ("GALAXY_TEST_AWS_ACCESS_KEY")
+@skip_unless_environ("GALAXY_TEST_AWS_SECRET_KEY")
+@skip_unless_environ("GALAXY_TEST_AWS_BUCKET")
+def test_aws_via_cloudbridge_store(tmp_path):
+    template_vars = {
+        "access_key": os.environ["GALAXY_TEST_AWS_ACCESS_KEY"],
+        "secret_key": os.environ["GALAXY_TEST_AWS_SECRET_KEY"],
+        "bucket": os.environ["GALAXY_TEST_AWS_BUCKET"],
+    }
+    with TestConfig(AMAZON_CLOUDBRIDGE_TEMPLATE_TEST_CONFIG_YAML, template_vars=template_vars) as (_, object_store):
+        # disabling get_object_url check - cloudbridge in this config assumes the region
+        # is us-east-1 and generates a URL for that region. This functionality works and can
+        # be tested if a region is specified in the configuration (see next config and test case).
+        verify_caching_object_store_functionality(tmp_path, object_store, check_get_url=False)
+
+
+AMAZON_CLOUDBRIDGE_WITH_REGION_TEMPLATE_TEST_CONFIG_YAML = """
+type: cloud
+store_by: uuid
+provider: aws
+auth:
+  access_key: ${access_key}
+  secret_key: ${secret_key}
+  region: ${region}
+
+bucket:
+  name: ${bucket}
+
+extra_dirs:
+- type: job_work
+  path: database/job_working_directory_azure
+- type: temp
+  path: database/tmp_azure
+"""
+
+
+@skip_unless_environ("GALAXY_TEST_AWS_ACCESS_KEY")
+@skip_unless_environ("GALAXY_TEST_AWS_SECRET_KEY")
+@skip_unless_environ("GALAXY_TEST_AWS_BUCKET")
+@skip_unless_environ("GALAXY_TEST_AWS_REGION")
+def test_aws_via_cloudbridge_store_with_region(tmp_path):
+    template_vars = {
+        "access_key": os.environ["GALAXY_TEST_AWS_ACCESS_KEY"],
+        "secret_key": os.environ["GALAXY_TEST_AWS_SECRET_KEY"],
+        "bucket": os.environ["GALAXY_TEST_AWS_BUCKET"],
+        "region": os.environ["GALAXY_TEST_AWS_REGION"],
+    }
+    with TestConfig(AMAZON_CLOUDBRIDGE_WITH_REGION_TEMPLATE_TEST_CONFIG_YAML, template_vars=template_vars) as (_, object_store):
+        verify_caching_object_store_functionality(tmp_path, object_store)
+
+
+
 class MockDataset:
     def __init__(self, id):
         self.id = id