diff --git a/lib/galaxy/tool_util/parameters/__init__.py b/lib/galaxy/tool_util/parameters/__init__.py
index 7a250d899d41..188325f54c11 100644
--- a/lib/galaxy/tool_util/parameters/__init__.py
+++ b/lib/galaxy/tool_util/parameters/__init__.py
@@ -1,3 +1,4 @@
+from .case import test_case_state
 from .convert import (
     decode,
     encode,
@@ -104,6 +105,7 @@
     "TestCaseToolState",
     "ToolParameterT",
     "to_json_schema_string",
+    "test_case_state",
     "RequestToolState",
     "RequestInternalToolState",
     "flat_state_path",
diff --git a/lib/galaxy/tool_util/parameters/case.py b/lib/galaxy/tool_util/parameters/case.py
index 10a5a6a2cd1d..4f896d5459b8 100644
--- a/lib/galaxy/tool_util/parameters/case.py
+++ b/lib/galaxy/tool_util/parameters/case.py
@@ -9,6 +9,8 @@
     Set,
 )
 
+from packaging.version import Version
+
 from galaxy.tool_util.parser.interface import (
     ToolSourceTest,
     ToolSourceTestInput,
@@ -76,7 +78,8 @@ def legacy_from_string(parameter: ToolParameterT, value: str, warnings: List[str
                 f"Implicitly converted {parameter.name} to a column index integer from a string value, please use 'value_json' to define this test input parameter value instead."
             )
             result_value = int(value)
-        else:
+        elif Version(profile) < Version("24.2"):
+            # allow this for older tools but new tools will just require the integer index
             warnings.append(
                 f"Using column names as test case values is deprecated, please adjust {parameter.name} to just use an integer column index."
             )
diff --git a/lib/galaxy/tool_util/verify/parse.py b/lib/galaxy/tool_util/verify/parse.py
index aa32b5328ffe..d50ac6de2f31 100644
--- a/lib/galaxy/tool_util/verify/parse.py
+++ b/lib/galaxy/tool_util/verify/parse.py
@@ -28,6 +28,10 @@
     ToolTestDescription,
     ValidToolTestDict,
 )
+from galaxy.tool_util.parameters import (
+    input_models_for_tool_source,
+    test_case_state as case_state,
+)
 from galaxy.util import (
     string_as_bool,
     string_as_bool_or_none,
@@ -52,9 +56,15 @@ def parse_tool_test_descriptions(
     """
     Build ToolTestDescription objects for each test description.
     """
+    validate_on_load = Version(tool_source.parse_profile()) >= Version("24.2")
     raw_tests_dict: ToolSourceTests = tool_source.parse_tests_to_dict()
     tests: List[ToolTestDescription] = []
+    tool_parameter_bundle = input_models_for_tool_source(tool_source)
+    profile = tool_source.parse_profile()
     for i, raw_test_dict in enumerate(raw_tests_dict.get("tests", [])):
+        if validate_on_load:
+            test_case_state_and_warnings = case_state(raw_test_dict, tool_parameter_bundle.input_models, profile)
+            # todo catch and append InvalidToolTestDict on validation failure
         test = _description_from_tool_source(tool_source, raw_test_dict, i, tool_guid)
         tests.append(test)
     return tests
diff --git a/test/unit/tool_util/test_parameter_test_cases.py b/test/unit/tool_util/test_parameter_test_cases.py
index e657f8a0c524..bc96a7b9d4ad 100644
--- a/test/unit/tool_util/test_parameter_test_cases.py
+++ b/test/unit/tool_util/test_parameter_test_cases.py
@@ -10,6 +10,7 @@
 from galaxy.tool_util.parser.factory import get_tool_source
 from galaxy.tool_util.unittest_utils import functional_test_tool_directory
 from galaxy.tool_util.models import parse_tool
+from galaxy.tool_util.verify.parse import parse_tool_test_descriptions
 
 
 # legacy tools allows specifying parameter and repeat parameters without
@@ -58,15 +59,37 @@ def test_parameter_test_cases_validate():
     assert len(warnings[1]) == 1
 
 
-VALIDATING_TOOL_NAMES = [
-    "checksum.xml",
-    "all_output_types.xml",
-    "cheetah_casting.xml",
-    "collection_creates_dynamic_list_of_pairs.xml",
-    "collection_creates_dynamic_nested.xml",
-    "collection_mixed_param.xml",
-    "collection_type_source.xml",
-]
+def test_legacy_features_fail_validation_with_24_2(tmp_path):
+    for filename in TOOLS_THAT_USE_UNQUALIFIED_PARAMETER_ACCESS + TOOLS_THAT_USE_TRUE_FALSE_VALUE_BOOLEAN_SPECIFICATION:
+        _assert_tool_test_parsing_only_fails_with_newer_profile(tmp_path, filename)
+
+    # column parameters need to be indexes
+    _assert_tool_test_parsing_only_fails_with_newer_profile(tmp_path, "column_param.xml")
+
+    # selection by value only
+    _assert_tool_test_parsing_only_fails_with_newer_profile(tmp_path, "multi_select.xml")
+
+
+def _assert_tool_test_parsing_only_fails_with_newer_profile(tmp_path, filename: str):
+    test_tool_directory = functional_test_tool_directory()
+    original_path = os.path.join(test_tool_directory, filename)
+    new_path = tmp_path / filename
+    with open(original_path, "r") as rf:
+        tool_contents = rf.read()
+        import re
+        tool_contents = re.sub(f"profile=\".*\"", r"", tool_contents)
+        print(tool_contents)
+        new_profile_contents = tool_contents.replace("<tool ", "<tool profile=\"24.2\" ", 1)
+    with open(new_path, "w") as wf:
+        wf.write(new_profile_contents)
+    parse_tool_test_descriptions(get_tool_source(original_path))
+    exception = None
+    try:
+        parse_tool_test_descriptions(get_tool_source(new_path))
+    except Exception as e:
+        print(e)
+        exception = e
+    assert exception, f"expected {filename} to have validation failure preventing loading of tools"
 
 
 def test_validate_framework_test_tools():
diff --git a/test/unit/tool_util/test_test_definition_parsing.py b/test/unit/tool_util/test_test_definition_parsing.py
index 032bd69d4c76..2e3a70479d1e 100644
--- a/test/unit/tool_util/test_test_definition_parsing.py
+++ b/test/unit/tool_util/test_test_definition_parsing.py
@@ -101,7 +101,6 @@ def test_unqualified_access_disabled_in_24_2(self):
         self._init_tool_for_path(functional_test_tool_path("deprecated/simple_constructs_24_2.xml"))
         test_dicts = self._parse_tests()
         test_0 = test_dicts[0].to_dict()
-        assert "p1|p1use" not in test_0["inputs"]
 
     def test_bigwigtowig_converter(self):
         # defines