CryoSPARC Reconstruction Parameters and Preprocessing Details

[JachyLikeCoding]

Hello，
I am currently using CryoSegNet segmentation results to perform cryoSPARC reconstruction, but I am unable to achieve the results reported in the paper. I would appreciate it if you could clarify a few details about the reconstruction process:

1. Did you use the default parameters in cryoSPARC for the reconstruction process, or were any parameters adjusted specifically for your dataset?
2. Was there any preprocessing done on the segmentation results before reconstruction in cryoSPARC? If so, could you provide some details on the preprocessing steps?

Thank you very much for your help and for sharing this valuable resource!

[rajangyawali]

Hi,

The default parameters are used. The extraction box size in 'Extract Micrographs job' is different for different proteins. No further preprocessing is required.

Thank you. Feel free to ask me if you have further questions