You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
Describe the bug
The number of bases to take from each file is calculated independently of whether there are enough bases available in each read. If there are not, the read is appended with N characters. Since words with N in them are skipped by HUMID, this gives zero usable reads.
Expected behavior
Since the files only contain a single read, there should also be a single read in the deduplicated output. Instead, we get no output at all, and the stats.dat file shows there were 0 usable reads.
Desktop (please complete the following information):
Using HUMID 1.0.2
The text was updated successfully, but these errors were encountered:
Describe the bug
The number of bases to take from each file is calculated independently of whether there are enough bases available in each read. If there are not, the read is appended with
N
characters. Sincewords
withN
in them are skipped by HUMID, this gives zero usable reads.To Reproduce
R1-no-umi_dedup.fastq.gz
R2_dedup.fastq.gz
R3_dedup.fastq.gz
Steps to reproduce the behavior:
Expected behavior
Since the files only contain a single read, there should also be a single read in the deduplicated output. Instead, we get no output at all, and the
stats.dat
file shows there were 0 usable reads.Desktop (please complete the following information):
The text was updated successfully, but these errors were encountered: