GC skew for plasmid genomes generates empty output file

[Jigyasa3]

Hi @jenniferlu717 ,

Thanks for the great software! I am interested in using it on plasmid genomes, but I end up getting an incorrect result with the shiny app, and no output with the locally downloaded skewi.py script.

The code for the skewi.py-
python /home/jigyasaa/downloads/SkewIT/src/skewi.py --plasmid -i ${IN_DIR}/NC_001621.1.fa -o NC_001621.1.fa_skewi.txt
This generates an empty file

The output from the shiny app-
This shows that the GC skew is ~20,001 bp. But the origin of replication for this plasmid is 11864..12943

Any suggestions?