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I created a new higlass-transcripts track from GeneCode_M31 in the following way:
cd ../data/ref/GeneCode_M31/higlass
curl https://ftp.ebi.ac.uk/pub/databases/gencode/Gencode_mouse/release_M31/gencode.vM31.primary_assembly.annotation.gtf.gz > gencode.vM31.primary_assembly.annotation.gtf.gz
gzip -d gencode.vM31.primary_assembly.annotation.gtf.gz
FILE=gencode.vM31.primary_assembly.annotation.gtf
BEDDB=gencode.vM31.primary_assembly_transcripts.beddb
PATH_SCRIPT=../../../../tools/higlass/higlass-transcripts # this repo was cloned here
CHRSIZE=mm39.chrom.mod.sizes
python $PATH_SCRIPT/scripts/extract_transcript_data.py --input-filename $FILE --chromsizes-filename $CHRSIZE -o $FILE.txt
clodius aggregate bedfile --max-per-tile 20 --importance-column 5 --chromsizes-filename $CHRSIZE --output-file $BEDDB --delimiter $'\t' $FILE.txt
# Finally I ingested the following way:
docker exec higlass-container python higlass-server/manage.py ingest_tileset --filename /tmp/gencode.vM31.primary_assembly_transcripts.beddb --filetype beddb --datatype gene-annotation --uid transcripts_abcd1234
But when I include this transcript track at the top in HiGlass then it is empty. If I try to modify the size of the track then HiGlass crashes and only a white page appears.
I created a new higlass-transcripts track from GeneCode_M31 in the following way:
But when I include this transcript track at the top in HiGlass then it is empty. If I try to modify the size of the track then HiGlass crashes and only a white page appears.
Note:
This was the "mm39.chrom.mod.sizes" file:
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