diff --git a/.readthedocs.yaml b/.readthedocs.yaml
index f57db3ed..8d6ffb00 100644
--- a/.readthedocs.yaml
+++ b/.readthedocs.yaml
@@ -6,9 +6,9 @@
 version: 2
 
 build:
-  os: ubuntu-20.04
+  os: ubuntu-22.04
   tools:
-    python: '3.9'
+    python: '3.12'
 
 # Build documentation in the docs/ directory with Sphinx
 sphinx:
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 78b5742d..ecf61732 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,6 +3,7 @@
 ## 1.0.0 (Upcoming)
 ### Enhancements
 * Added support for Pathlib paths. @mavaylon1 [#212](https://github.com/hdmf-dev/hdmf-zarr/pull/212)
+* Updated packages used for testing and readthedocs configuration. @mavaylon1, @rly [#214](https://github.com/hdmf-dev/hdmf-zarr/pull/214)
 
 ## 0.9.0 (September 16, 2024)
 ### Enhancements
diff --git a/requirements-dev.txt b/requirements-dev.txt
index 0fd19af4..047125eb 100644
--- a/requirements-dev.txt
+++ b/requirements-dev.txt
@@ -12,4 +12,4 @@ pytest==7.4.3
 pytest-cov==4.1.0
 python-dateutil==2.8.2
 ruff==0.1.3
-tox==4.11.3
\ No newline at end of file
+tox==4.11.3
diff --git a/requirements-opt.txt b/requirements-opt.txt
index 771f5d04..6fc3bcb0 100644
--- a/requirements-opt.txt
+++ b/requirements-opt.txt
@@ -1,3 +1,3 @@
-tqdm==4.66.4
-fsspec==2024.6.0
-s3fs==2024.6.0
+tqdm==4.67.0
+fsspec==2024.10.0
+s3fs==2024.10.0
diff --git a/requirements.txt b/requirements.txt
index 338c96f2..840b4815 100644
--- a/requirements.txt
+++ b/requirements.txt
@@ -1,7 +1,7 @@
 # pinned dependencies to reproduce an entire development environment to use HDMF-ZARR
-hdmf==3.14.2
-zarr==2.16.1
-pynwb==2.5.0
-numpy==2.0.0
-numcodecs==0.12.1
-threadpoolctl==3.2.0
+hdmf==3.14.5
+zarr==2.18.3
+pynwb==2.8.2
+numpy==2.1.3
+numcodecs==0.13.1
+threadpoolctl==3.5.0