From c6ea913129831383bc116998e1de000c333674ac Mon Sep 17 00:00:00 2001 From: Lorena Pantano Date: Tue, 17 Dec 2024 10:15:49 -0500 Subject: [PATCH] Update atacseq notes --- docs/pipelines.md | 3 +++ 1 file changed, 3 insertions(+) diff --git a/docs/pipelines.md b/docs/pipelines.md index abbd00c..4938c8f 100644 --- a/docs/pipelines.md +++ b/docs/pipelines.md @@ -36,6 +36,9 @@ NFR peaks `nf-core-atac-seq_shift_NFR`: - `--minFragmentLength 0` - `--maxFragmentLength 120` +**Note**: Recommendation to check the fragment length distribution after the run to make sure you're capturing the NFRs. +**Note**: We don't need the MN(180, 247), DN (315, 473) and TN (558, 615), unless it's a specific case where we are looking at global shifts in accessibility + ## Nextflow in Seqera platform - Create an user here: https://cloud.seqera.io/login