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MRXS: conversion is incorrect when multiple pyramids are present #258

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melissalinkert opened this issue Sep 26, 2024 · 1 comment
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Reported in https://forum.image.sc/t/issues-mrxs-to-tiff-using-bioformat2raw/100695; private sample data is in automated-tests/inbox/imagesc-100695/.

The problematic dataset has an extra Slide zoom mask level inserted between the Slide zoom level and Slide filter level (the "real" data). Looking at a diff of Slidedat.ini against the Slidedat.ini for https://openslide.cs.cmu.edu/download/openslide-testdata/Mirax/CMU-1.zip is useful.

melissalinkert@905daf4 is a start at fixing the channel count detection, but that does not result in correct image data. I suspect that pyramidDepth needs to be calculated and recorded separately for each hierarchy so that the index file is read properly, but more investigation is needed.

@melissalinkert melissalinkert added the bug Something isn't working label Sep 26, 2024
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https://forum.image.sc/t/help-with-conversion-of-mrxs-files-to-ome-tiff/104549 (data in https://zenodo.org/records/14029699) appears to be related. The channel count is incorrect (2, when it should be 3), and the converted image is entirely black.

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