diff --git a/lib/galaxy/config/sample/object_store_conf.sample.yml b/lib/galaxy/config/sample/object_store_conf.sample.yml
index b1b2cb34afec..5fdfcb8d8ed1 100644
--- a/lib/galaxy/config/sample/object_store_conf.sample.yml
+++ b/lib/galaxy/config/sample/object_store_conf.sample.yml
@@ -135,10 +135,64 @@ backends:
     store_by: uuid
     files_dir: /old-fs/galaxy/files
 
+
+# There are now four ways to access S3 related services. Two are
+# suitable just for AWS services (aws_s3 & cloud), one is
+# more suited for non-AWS S3 compatible services (generic_s3),
+# and finally boto3 gracefully handles either scenario.
+# 
+# boto3 is built on the newest and most widely used Python client
+# outside of Galaxy. It has advanced transfer options and is likely
+# the client you should use for new setup. generic_s3 and aws_s3
+# have existed in Galaxy for longer and could perhaps be considered
+# more battle tested. Both boto3 and generic_s3 have been tested
+# with multiple non-AWS APIs including minio and GCP. The cloud
+# implementation is based on CloudBridge and is still supported
+# and has been recently tested - the downside is mostly the advanced
+# multi-threaded processing options of boto3 are not available
+# and it has not been battle tested like aws_s3.
+
+#
+# Sample AWS S3 Object Store configuration (newest boto3 client)
+#
+type: boto3
+auth:
+  access_key: ...
+  secret_key: ...
+bucket:
+  name: unique_bucket_name_all_lowercase
+connection:  # not strictly needed but more of the API works with this.
+  region: us-east-1
+transfer:
+  multipart_threshold: 10000000
+  download_max_concurrency: 5
+  upload_max_concurrency: 10
+  # any of these options:
+  # multipart_threshold, max_concurrency, multipart_chunksize,
+  # num_download_attempts, max_io_queue, io_chunksize, use_threads,
+  # and max_bandwidth
+  # can be set. By default they will apply to uploads and downloads
+  # but they can be prefixed with upload_ or download_ as shown above
+  # to apply to just one scenario. More information about these parameters
+  # can be found at:
+  # https://boto3.amazonaws.com/v1/documentation/api/latest/reference/customizations/s3.html#boto3.s3.transfer.TransferConfig
+
+cache:
+  path: database/object_store_cache_s3
+  size: 1000
+  cache_updated_data: true
+extra_dirs:
+  - type: job_work
+    path: database/job_working_directory_s3
+
+
+
 #
-# Sample AWS S3 Object Store configuration
+# Sample AWS S3 Object Store configuration (legacy boto implementation)
 #
-
+# This implementation will use axel automatically for file transfers if it is on
+# Galaxy's path. Otherwise, it will use various python-based strategies for multi-part
+# upload of large uploads but all downloads will be single threaded.
 type: aws_s3
 auth:
   access_key: ...
@@ -147,6 +201,8 @@ bucket:
   name: unique_bucket_name_all_lowercase
   use_reduced_redundancy: false
   max_chunk_size: 250
+connection:  # not strictly needed but more of the API works with this.
+  region: us-east-1
 cache:
   path: database/object_store_cache_s3
   size: 1000
@@ -182,7 +238,30 @@ extra_dirs:
     path: database/job_working_directory_irods
 
 #
-# Sample non-AWS S3 Object Store (e.g. swift) configuration
+# Sample non-AWS S3 Object Store (e.g. swift) configuration (boto3)
+#
+
+type: boto3
+auth:
+  access_key: ...
+  secret_key: ...
+bucket:
+  name: unique_bucket_name_all_lowercase
+connection:
+  endpoint_url: https://swift.example.org:6000/
+  # region: some services may make use of region is specified.
+cache:
+  path: database/object_store_cache_swift
+  size: 1000
+  cache_updated_data: true
+# transfer:   # see transfer options for boto3 above in AWS configuration.
+extra_dirs:
+  - type: job_work
+    path: database/job_working_directory_swift
+
+
+#
+# Sample non-AWS S3 Object Store (e.g. swift) configuration (legacy boto client)
 #
 
 type: generic_s3