diff --git a/.circleci/config.yml b/.circleci/config.yml
index b8d9bb466c21..f29c69235e68 100644
--- a/.circleci/config.yml
+++ b/.circleci/config.yml
@@ -15,7 +15,7 @@ variables:
 jobs:
   get_code:
     docker:
-      - image: cimg/python:3.7
+      - image: cimg/python:3.8
     <<: *set_workdir
     steps:
       # Replace standard code checkout with shallow clone to speed things up.
@@ -73,7 +73,7 @@ jobs:
             - ~/repo
   validate_test_tools:
     docker:
-      - image: cimg/python:3.7
+      - image: cimg/python:3.8
     <<: *set_workdir
     steps:
       - *restore_repo_cache
diff --git a/.github/workflows/api.yaml b/.github/workflows/api.yaml
index 90038e328865..753f669f148f 100644
--- a/.github/workflows/api.yaml
+++ b/.github/workflows/api.yaml
@@ -28,7 +28,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
         chunk: [0, 1]
     services:
       postgres:
diff --git a/.github/workflows/check_test_class_names.yaml b/.github/workflows/check_test_class_names.yaml
index 92db607c7bdb..217995df93e4 100644
--- a/.github/workflows/check_test_class_names.yaml
+++ b/.github/workflows/check_test_class_names.yaml
@@ -11,7 +11,7 @@ jobs:
     runs-on: ubuntu-latest
     strategy:
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
     steps:
       - uses: actions/checkout@v3
       - uses: actions/setup-python@v4
diff --git a/.github/workflows/converter_tests.yaml b/.github/workflows/converter_tests.yaml
index 359edf8b0d9e..1d8c0ee963b8 100644
--- a/.github/workflows/converter_tests.yaml
+++ b/.github/workflows/converter_tests.yaml
@@ -22,7 +22,7 @@ jobs:
     runs-on: ubuntu-latest
     strategy:
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
     steps:
       - if: github.event_name == 'schedule'
         run: |
diff --git a/.github/workflows/cwl_conformance.yaml b/.github/workflows/cwl_conformance.yaml
index 716a8ca2bbbd..206bd33798a1 100644
--- a/.github/workflows/cwl_conformance.yaml
+++ b/.github/workflows/cwl_conformance.yaml
@@ -22,7 +22,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
         marker: ['green', 'red and required', 'red and not required']
         conformance-version: ['cwl_conformance_v1_0'] #, 'cwl_conformance_v1_1', 'cwl_conformance_v1_2']
     services:
diff --git a/.github/workflows/db_indexes.yaml b/.github/workflows/db_indexes.yaml
index af8455edd887..88f1d02297af 100644
--- a/.github/workflows/db_indexes.yaml
+++ b/.github/workflows/db_indexes.yaml
@@ -24,11 +24,11 @@ jobs:
       matrix:
         db: ['postgresql', 'sqlite']
         postgresql-version: ['13']
-        python-version: ['3.7']
+        python-version: ['3.8']
         include:
           - db: postgresql
             postgresql-version: '9.6'
-            python-version: '3.7'
+            python-version: '3.8'
     services:
       postgres:
         image: postgres:${{ matrix.postgresql-version }}
diff --git a/.github/workflows/dependencies.yaml b/.github/workflows/dependencies.yaml
index 0d02ae2f1234..31405cc13498 100644
--- a/.github/workflows/dependencies.yaml
+++ b/.github/workflows/dependencies.yaml
@@ -9,17 +9,12 @@ jobs:
     runs-on: ubuntu-latest
     strategy:
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
     steps:
       - uses: actions/checkout@v3
       - uses: actions/setup-python@v4
         with:
-          # poetry requires Python >=3.8, but lint requirements currently need
-          # to be generated with `pip freeze`` on the oldest Python version
-          # supported by Galaxy.
-          python-version: |
-            ${{ matrix.python-version }}
-            3.8
+          python-version: ${{ matrix.python-version }}
       - name: Update dependencies
         run: | 
           python -m venv .venv
diff --git a/.github/workflows/deployment.yaml b/.github/workflows/deployment.yaml
index 441f35e71673..082e93d9328d 100644
--- a/.github/workflows/deployment.yaml
+++ b/.github/workflows/deployment.yaml
@@ -33,7 +33,7 @@ jobs:
     runs-on: ubuntu-latest
     strategy:
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
     steps:
       - uses: actions/checkout@v3
         with:
diff --git a/.github/workflows/docs.yaml b/.github/workflows/docs.yaml
index 6de9bf997964..9f67f3322488 100644
--- a/.github/workflows/docs.yaml
+++ b/.github/workflows/docs.yaml
@@ -16,7 +16,7 @@ jobs:
     runs-on: ubuntu-latest
     strategy:
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
     steps:
       - name: Get target branch name (push)
         if: github.event_name == 'push'
diff --git a/.github/workflows/first_startup.yaml b/.github/workflows/first_startup.yaml
index 34093cae9605..10f506dbbedf 100644
--- a/.github/workflows/first_startup.yaml
+++ b/.github/workflows/first_startup.yaml
@@ -21,7 +21,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7', '3.11']
+        python-version: ['3.8', '3.11']
     defaults:
       run:
         shell: bash -l {0}
diff --git a/.github/workflows/framework.yaml b/.github/workflows/framework.yaml
index 5de51cca7cad..53a287c5d7c0 100644
--- a/.github/workflows/framework.yaml
+++ b/.github/workflows/framework.yaml
@@ -25,7 +25,7 @@ jobs:
     runs-on: ubuntu-latest
     strategy:
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
     services:
       postgres:
         image: postgres:13
diff --git a/.github/workflows/integration.yaml b/.github/workflows/integration.yaml
index d3660517fb72..58aa81dd63c3 100644
--- a/.github/workflows/integration.yaml
+++ b/.github/workflows/integration.yaml
@@ -29,7 +29,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
         chunk: ['0', '1', '2', '3']
     services:
       postgres:
diff --git a/.github/workflows/integration_selenium.yaml b/.github/workflows/integration_selenium.yaml
index e8cf74fb5639..427a41ec1559 100644
--- a/.github/workflows/integration_selenium.yaml
+++ b/.github/workflows/integration_selenium.yaml
@@ -29,7 +29,7 @@ jobs:
     runs-on: ubuntu-latest
     strategy:
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
     services:
       postgres:
         image: postgres:13
diff --git a/.github/workflows/lint.yaml b/.github/workflows/lint.yaml
index 590f93cd5e2c..037f343d9ee9 100644
--- a/.github/workflows/lint.yaml
+++ b/.github/workflows/lint.yaml
@@ -22,7 +22,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7', '3.11']
+        python-version: ['3.8', '3.11']
     env:
       LINT_PATH: 'lib/galaxy/dependencies/pinned-lint-requirements.txt'
       TYPE_PATH: 'lib/galaxy/dependencies/pinned-typecheck-requirements.txt'
diff --git a/.github/workflows/lint_openapi_schema.yml b/.github/workflows/lint_openapi_schema.yml
index 32ddedf5cd7d..c327fabe72e8 100644
--- a/.github/workflows/lint_openapi_schema.yml
+++ b/.github/workflows/lint_openapi_schema.yml
@@ -20,7 +20,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7', '3.11']
+        python-version: ['3.8', '3.11']
     steps:
       - uses: actions/checkout@v3
         with:
diff --git a/.github/workflows/mulled.yaml b/.github/workflows/mulled.yaml
index a9e84ef28a5e..1b140eb1f6c3 100644
--- a/.github/workflows/mulled.yaml
+++ b/.github/workflows/mulled.yaml
@@ -20,7 +20,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
     steps:
       - uses: actions/checkout@v3
         with:
diff --git a/.github/workflows/osx_startup.yaml b/.github/workflows/osx_startup.yaml
index 0015076712b6..d550c6741678 100644
--- a/.github/workflows/osx_startup.yaml
+++ b/.github/workflows/osx_startup.yaml
@@ -18,7 +18,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7', '3.11']
+        python-version: ['3.8', '3.11']
     defaults:
       run:
         shell: bash -l {0}
diff --git a/.github/workflows/performance.yaml b/.github/workflows/performance.yaml
index 4eda60399585..48287a739f8c 100644
--- a/.github/workflows/performance.yaml
+++ b/.github/workflows/performance.yaml
@@ -24,7 +24,7 @@ jobs:
     runs-on: ubuntu-latest
     strategy:
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
     services:
       postgres:
         image: postgres:13
diff --git a/.github/workflows/publish_artifacts.yaml b/.github/workflows/publish_artifacts.yaml
index fd4d736f1b34..3703d932c0cc 100644
--- a/.github/workflows/publish_artifacts.yaml
+++ b/.github/workflows/publish_artifacts.yaml
@@ -9,7 +9,7 @@ jobs:
     runs-on: ubuntu-latest
     strategy:
         matrix:
-            python-version: ['3.7']
+            python-version: ['3.8']
     steps:
       - uses: actions/setup-python@v4
         with:
diff --git a/.github/workflows/reports_startup.yaml b/.github/workflows/reports_startup.yaml
index 805686e3d388..5597489d3726 100644
--- a/.github/workflows/reports_startup.yaml
+++ b/.github/workflows/reports_startup.yaml
@@ -18,7 +18,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7', '3.11']
+        python-version: ['3.8', '3.11']
     defaults:
       run:
         shell: bash -l {0}
diff --git a/.github/workflows/selenium.yaml b/.github/workflows/selenium.yaml
index 11cf1886fc2b..b8235cb7869f 100644
--- a/.github/workflows/selenium.yaml
+++ b/.github/workflows/selenium.yaml
@@ -30,7 +30,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
         chunk: [0, 1, 2]
     services:
       postgres:
diff --git a/.github/workflows/test_galaxy_packages.yaml b/.github/workflows/test_galaxy_packages.yaml
index 089d4f45f19e..f2e5d88b52a7 100644
--- a/.github/workflows/test_galaxy_packages.yaml
+++ b/.github/workflows/test_galaxy_packages.yaml
@@ -18,7 +18,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7', '3.11']
+        python-version: ['3.8', '3.11']
     steps:
       - uses: actions/checkout@v3
         with:
diff --git a/.github/workflows/test_galaxy_packages_for_pulsar.yaml b/.github/workflows/test_galaxy_packages_for_pulsar.yaml
index 7f5f5499bc4c..2a8e39cadbce 100644
--- a/.github/workflows/test_galaxy_packages_for_pulsar.yaml
+++ b/.github/workflows/test_galaxy_packages_for_pulsar.yaml
@@ -20,7 +20,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.7']  # don't upgrade, see https://github.com/galaxyproject/galaxy/pull/16649
     steps:
       - uses: actions/checkout@v3
         with:
diff --git a/.github/workflows/toolshed.yaml b/.github/workflows/toolshed.yaml
index 4e3a2b2cac8c..95db16b404ac 100644
--- a/.github/workflows/toolshed.yaml
+++ b/.github/workflows/toolshed.yaml
@@ -21,7 +21,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7', '3.11']
+        python-version: ['3.8', '3.11']
         shed-api: ['v1', 'v2']
         test-install-client: ['galaxy_api', 'standalone']
     services:
diff --git a/.github/workflows/unit-postgres.yaml b/.github/workflows/unit-postgres.yaml
index 6e695fd64082..f2c57b6228d4 100644
--- a/.github/workflows/unit-postgres.yaml
+++ b/.github/workflows/unit-postgres.yaml
@@ -22,7 +22,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7']
+        python-version: ['3.8']
     services:
       postgres:
         image: postgres:13
diff --git a/.github/workflows/unit.yaml b/.github/workflows/unit.yaml
index 8ade16541a1f..9c32eae057d6 100644
--- a/.github/workflows/unit.yaml
+++ b/.github/workflows/unit.yaml
@@ -20,7 +20,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.7', '3.11']
+        python-version: ['3.8', '3.11']
     steps:
       - uses: actions/checkout@v3
         with:
diff --git a/README.rst b/README.rst
index 34d382dc68bb..17e11d9ff098 100644
--- a/README.rst
+++ b/README.rst
@@ -24,12 +24,12 @@ Community support is available at `Galaxy Help <https://help.galaxyproject.org/>
 Galaxy Quickstart
 =================
 
-Galaxy requires Python 3.7 . To check your Python version, run:
+Galaxy requires Python 3.8 . To check your Python version, run:
 
 .. code:: console
 
     $ python -V
-    Python 3.7.6
+    Python 3.8.18
 
 Start Galaxy:
 
diff --git a/doc/source/admin/python.md b/doc/source/admin/python.md
index 23870548c8e3..015f85e08a10 100644
--- a/doc/source/admin/python.md
+++ b/doc/source/admin/python.md
@@ -1,6 +1,6 @@
 # Supported Python versions
 
-Galaxy's core functionality is currently supported on Python **>=3.7** .
+Galaxy's core functionality is currently supported on Python **>=3.8** .
 
 If Galaxy complains about the version of Python you are using:
 
diff --git a/doc/source/dev/debugging_tests.md b/doc/source/dev/debugging_tests.md
index 231747572be7..6bda2d5c92f8 100644
--- a/doc/source/dev/debugging_tests.md
+++ b/doc/source/dev/debugging_tests.md
@@ -52,7 +52,7 @@ index 6647588dfb..c8d82957a1 100644
 @@ -14,7 +14,7 @@ jobs:
        fail-fast: false
        matrix:
-         python-version: ['3.7']
+         python-version: ['3.8']
 -        subset: ['upload_datatype', 'extended_metadata', 'kubernetes', 'not (upload_datatype or extended_metadata or kubernetes)']
 +        subset: ['not (upload_datatype or extended_metadata or kubernetes)']
      services:
diff --git a/lib/galaxy/app_unittest_utils/tools_support.py b/lib/galaxy/app_unittest_utils/tools_support.py
index 77c759499d75..6c94d5eb59da 100644
--- a/lib/galaxy/app_unittest_utils/tools_support.py
+++ b/lib/galaxy/app_unittest_utils/tools_support.py
@@ -123,7 +123,7 @@ class MockContext:
     def __init__(self, model_objects=None):
         self.expunged_all = False
         self.flushed = False
-        self.model_objects = model_objects or defaultdict(lambda: {})
+        self.model_objects = model_objects or defaultdict(dict)
         self.created_objects = []
         self.current = self
 
diff --git a/lib/galaxy/celery/tasks.py b/lib/galaxy/celery/tasks.py
index b94cc8f832b4..828e692ba0a2 100644
--- a/lib/galaxy/celery/tasks.py
+++ b/lib/galaxy/celery/tasks.py
@@ -68,12 +68,12 @@
 log = get_logger(__name__)
 
 
-@lru_cache()
+@lru_cache
 def setup_data_table_manager(app):
     app._configure_tool_data_tables(from_shed_config=False)
 
 
-@lru_cache()
+@lru_cache
 def cached_create_tool_from_representation(app, raw_tool_source):
     return create_tool_from_representation(
         app=app, raw_tool_source=raw_tool_source, tool_dir="", tool_source_class="XmlToolSource"
diff --git a/lib/galaxy/datatypes/text.py b/lib/galaxy/datatypes/text.py
index 91ea44cd3b3a..e7670856665b 100644
--- a/lib/galaxy/datatypes/text.py
+++ b/lib/galaxy/datatypes/text.py
@@ -6,6 +6,7 @@
 import logging
 import os
 import re
+import shlex
 import subprocess
 import tempfile
 from typing import (
@@ -38,7 +39,6 @@
 )
 from galaxy.util import (
     nice_size,
-    shlex_join,
     string_as_bool,
     unicodify,
 )
@@ -242,7 +242,7 @@ def _display_data_trusted(
                 ofilename = dataset.get_file_name()
                 log.exception(
                     'Command "%s" failed. Could not convert the Jupyter Notebook to HTML, defaulting to plain text.',
-                    shlex_join(cmd),
+                    shlex.join(cmd),
                 )
             return open(ofilename, mode="rb"), headers
 
diff --git a/lib/galaxy/datatypes/util/maf_utilities.py b/lib/galaxy/datatypes/util/maf_utilities.py
index bbfdf7967f51..52c18931c3c9 100644
--- a/lib/galaxy/datatypes/util/maf_utilities.py
+++ b/lib/galaxy/datatypes/util/maf_utilities.py
@@ -658,9 +658,6 @@ def get_starts_ends_fields_from_gene_bed(line):
     if len(fields) < 12:
         raise Exception(f"Not a proper 12 column BED line ({line}).")
     tx_start = int(fields[1])
-    strand = fields[5]
-    if strand != "-":
-        strand = "+"  # Default strand is +
     cds_start = int(fields[6])
     cds_end = int(fields[7])
 
diff --git a/lib/galaxy/dependencies/update.sh b/lib/galaxy/dependencies/update.sh
index 7771ea30791e..01708a54cfe4 100755
--- a/lib/galaxy/dependencies/update.sh
+++ b/lib/galaxy/dependencies/update.sh
@@ -6,7 +6,7 @@
 
 set -e
 
-SUPPORTED_PYTHON_VERSIONS="3.7 3.8 3.9 3.10 3.11"
+SUPPORTED_PYTHON_VERSIONS="3.8 3.9 3.10 3.11"
 NOT_SUPPORTED_NEXT_PYTHON_VERSION="3.12"
 
 this_directory="$(cd "$(dirname "$0")" > /dev/null && pwd)"
diff --git a/lib/galaxy/dependencies/update_lint_requirements.sh b/lib/galaxy/dependencies/update_lint_requirements.sh
index 6f06089aabf8..0225d931d11d 100755
--- a/lib/galaxy/dependencies/update_lint_requirements.sh
+++ b/lib/galaxy/dependencies/update_lint_requirements.sh
@@ -11,7 +11,7 @@ THIS_DIRECTORY="$(cd "$(dirname "$0")" > /dev/null && pwd)"
 
 update_pinned_reqs() {
     VENV=$(mktemp -d "${TMPDIR:-/tmp}/$1_venv.XXXXXXXXXX")
-    python3.7 -m venv "${VENV}"
+    python3.8 -m venv "${VENV}"
     . "${VENV}/bin/activate"
     pip install --upgrade pip setuptools
     pip install -r "${THIS_DIRECTORY}/$1-requirements.txt"
diff --git a/lib/galaxy/managers/notification.py b/lib/galaxy/managers/notification.py
index 0db48f7bfb3e..b0da5b1b9d8e 100644
--- a/lib/galaxy/managers/notification.py
+++ b/lib/galaxy/managers/notification.py
@@ -409,7 +409,7 @@ def resolve_users(self, recipients: NotificationRecipients) -> List[User]:
         user_ids_from_roles_stmt = self._get_all_user_ids_from_roles_query(all_role_ids)
 
         union_stmt = union(user_ids_from_groups_stmt, user_ids_from_roles_stmt)
-        user_ids_from_groups_and_roles = set([id for id, in self.sa_session.execute(union_stmt)])
+        user_ids_from_groups_and_roles = {id for id, in self.sa_session.execute(union_stmt)}
         unique_user_ids.update(user_ids_from_groups_and_roles)
 
         stmt = select(User).where(User.id.in_(unique_user_ids))
@@ -448,7 +448,7 @@ def _expand_group_and_roles_ids(self, group_ids: Set[int], role_ids: Set[int]) -
                 .where(GroupRoleAssociation.role_id.in_(role_ids))
                 .distinct()
             )
-            group_ids_from_roles = set([id for id, in self.sa_session.execute(stmt) if id is not None])
+            group_ids_from_roles = {id for id, in self.sa_session.execute(stmt) if id is not None}
             new_group_ids = group_ids_from_roles - processed_group_ids
 
             # Get role IDs associated with any of the given group IDs
@@ -458,7 +458,7 @@ def _expand_group_and_roles_ids(self, group_ids: Set[int], role_ids: Set[int]) -
                 .where(GroupRoleAssociation.group_id.in_(group_ids))
                 .distinct()
             )
-            role_ids_from_groups = set([id for id, in self.sa_session.execute(stmt) if id is not None])
+            role_ids_from_groups = {id for id, in self.sa_session.execute(stmt) if id is not None}
             new_role_ids = role_ids_from_groups - processed_role_ids
 
             # Stop if there are no new group or role IDs to process
diff --git a/lib/galaxy/tool_util/deps/conda_util.py b/lib/galaxy/tool_util/deps/conda_util.py
index f91203923cd2..3c15a8800b97 100644
--- a/lib/galaxy/tool_util/deps/conda_util.py
+++ b/lib/galaxy/tool_util/deps/conda_util.py
@@ -5,6 +5,7 @@
 import os
 import platform
 import re
+import shlex
 import shutil
 import sys
 import tempfile
@@ -28,7 +29,6 @@
     commands,
     download_to_file,
     listify,
-    shlex_join,
     smart_str,
     which,
 )
@@ -253,7 +253,7 @@ def exec_command(self, operation: str, args: List[str], stdout_path: Optional[st
         env = {}
         if self.condarc_override:
             env["CONDARC"] = self.condarc_override
-        cmd_string = shlex_join(cmd)
+        cmd_string = shlex.join(cmd)
         kwds = dict()
         try:
             if stdout_path:
diff --git a/lib/galaxy/tool_util/deps/mulled/mulled_build.py b/lib/galaxy/tool_util/deps/mulled/mulled_build.py
index d2f6e0dc0090..839e9e3d8f0a 100644
--- a/lib/galaxy/tool_util/deps/mulled/mulled_build.py
+++ b/lib/galaxy/tool_util/deps/mulled/mulled_build.py
@@ -12,6 +12,7 @@
 import json
 import logging
 import os
+import shlex
 import shutil
 import stat
 import string
@@ -38,7 +39,6 @@
     commands,
     download_to_file,
     safe_makedirs,
-    shlex_join,
     unicodify,
 )
 from ._cli import arg_parser
@@ -335,7 +335,7 @@ def mull_targets(
             involucro_args.insert(6, "-set")
             involucro_args.insert(7, f"TEST_BINDS={','.join(test_bind)}")
     cmd = involucro_context.build_command(involucro_args)
-    print(f"Executing: {shlex_join(cmd)}")
+    print(f"Executing: {shlex.join(cmd)}")
     if dry_run:
         return 0
     ensure_installed(involucro_context, True)
diff --git a/lib/galaxy/tools/imp_exp/export_history.py b/lib/galaxy/tools/imp_exp/export_history.py
index 4127d11770bb..340c19a203d4 100644
--- a/lib/galaxy/tools/imp_exp/export_history.py
+++ b/lib/galaxy/tools/imp_exp/export_history.py
@@ -40,9 +40,6 @@ def main(argv=None):
     )
     parser.add_option("--file-sources", type=str, help="file sources json")
     (options, args) = parser.parse_args(argv)
-    galaxy_version = options.galaxy_version
-    if galaxy_version is None:
-        galaxy_version = "19.05"
 
     gzip = bool(options.gzip)
     assert len(args) >= 2
diff --git a/lib/galaxy/util/__init__.py b/lib/galaxy/util/__init__.py
index 2db3e454bb22..0f028eb90b1f 100644
--- a/lib/galaxy/util/__init__.py
+++ b/lib/galaxy/util/__init__.py
@@ -13,7 +13,6 @@
 import os
 import random
 import re
-import shlex
 import shutil
 import smtplib
 import stat
@@ -96,14 +95,6 @@ def __new__(cls, element=None, file=None) -> etree.ElementTree:
 )
 from .rst_to_html import rst_to_html  # noqa: F401
 
-try:
-    shlex_join = shlex.join  # type: ignore[attr-defined]
-except AttributeError:
-    # Python < 3.8
-    def shlex_join(split_command):
-        return " ".join(map(shlex.quote, split_command))
-
-
 inflector = Inflector()
 
 log = get_logger(__name__)
diff --git a/lib/galaxy/util/path/__init__.py b/lib/galaxy/util/path/__init__.py
index a82d6619a0a3..744fed27dc65 100644
--- a/lib/galaxy/util/path/__init__.py
+++ b/lib/galaxy/util/path/__init__.py
@@ -388,7 +388,7 @@ def external_chown(path, pwent, external_chown_script, description="file"):
 
         cmd = shlex.split(external_chown_script)
         cmd.extend([path, pwent[0], str(pwent[3])])
-        log.debug(f"Changing ownership of {path} with: '{galaxy.util.shlex_join(cmd)}'")
+        log.debug(f"Changing ownership of {path} with: '{shlex.join(cmd)}'")
         galaxy.util.commands.execute(cmd)
         return True
     except galaxy.util.commands.CommandLineException as e:
diff --git a/lib/galaxy/webapps/galaxy/services/notifications.py b/lib/galaxy/webapps/galaxy/services/notifications.py
index 6ca78fafb568..12c86a218046 100644
--- a/lib/galaxy/webapps/galaxy/services/notifications.py
+++ b/lib/galaxy/webapps/galaxy/services/notifications.py
@@ -134,7 +134,7 @@ def update_user_notification(
     ):
         """Updates a single notification received by the user with the requested values."""
         self.notification_manager.ensure_notifications_enabled()
-        updated_response = self.update_user_notifications(user_context, set([notification_id]), request)
+        updated_response = self.update_user_notifications(user_context, {notification_id}, request)
         if not updated_response.updated_count:
             self._raise_notification_not_found(notification_id)
 
diff --git a/packages/app/setup.cfg b/packages/app/setup.cfg
index c8cc845deed1..d9b9de4708cf 100644
--- a/packages/app/setup.cfg
+++ b/packages/app/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -77,7 +76,7 @@ install_requires =
     WebOb
     Whoosh
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.entry_points]
 console_scripts =
diff --git a/packages/auth/setup.cfg b/packages/auth/setup.cfg
index cca3a9b7ad7c..5dfbe3c629e2 100644
--- a/packages/auth/setup.cfg
+++ b/packages/auth/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -35,8 +34,8 @@ install_requires =
     galaxy-data
     galaxy-util
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.packages.find]
 exclude =
-    tests*
\ No newline at end of file
+    tests*
diff --git a/packages/config/setup.cfg b/packages/config/setup.cfg
index 16d2815d2a2e..a4bbe8efb729 100644
--- a/packages/config/setup.cfg
+++ b/packages/config/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -38,7 +37,7 @@ install_requires =
     pykwalify
     PyYAML
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.entry_points]
 console_scripts =
@@ -46,4 +45,4 @@ console_scripts =
 
 [options.packages.find]
 exclude =
-    tests*
\ No newline at end of file
+    tests*
diff --git a/packages/data/setup.cfg b/packages/data/setup.cfg
index 02f74386b9d5..b3374574ed65 100644
--- a/packages/data/setup.cfg
+++ b/packages/data/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -61,7 +60,7 @@ install_requires =
     typing-extensions
     WebOb
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.entry_points]
 console_scripts =
diff --git a/packages/files/setup.cfg b/packages/files/setup.cfg
index 9f89c257b476..3f7f01acaf1b 100644
--- a/packages/files/setup.cfg
+++ b/packages/files/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -36,7 +35,7 @@ install_requires =
     fs
     typing-extensions
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.packages.find]
 exclude =
diff --git a/packages/job_execution/setup.cfg b/packages/job_execution/setup.cfg
index ea1943235b8a..1d77581d51ff 100644
--- a/packages/job_execution/setup.cfg
+++ b/packages/job_execution/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -40,7 +39,7 @@ install_requires =
     MarkupSafe
     SQLAlchemy>=1.4.25,<2
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.entry_points]
 console_scripts =
@@ -49,4 +48,4 @@ console_scripts =
 
 [options.packages.find]
 exclude =
-    tests*
\ No newline at end of file
+    tests*
diff --git a/packages/job_metrics/setup.cfg b/packages/job_metrics/setup.cfg
index d9576a25c86d..80b93417f476 100644
--- a/packages/job_metrics/setup.cfg
+++ b/packages/job_metrics/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -34,8 +33,8 @@ include_package_data = True
 install_requires =
     galaxy-util
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.packages.find]
 exclude =
-    tests*
\ No newline at end of file
+    tests*
diff --git a/packages/meta/setup.cfg b/packages/meta/setup.cfg
index 516107f5088d..e3d3a152e651 100644
--- a/packages/meta/setup.cfg
+++ b/packages/meta/setup.cfg
@@ -10,7 +10,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -34,7 +33,7 @@ version = 22.5.0.dev0
 [options]
 include_package_data = True
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.extras_require]
 postgresql =
diff --git a/packages/navigation/setup.cfg b/packages/navigation/setup.cfg
index 3b16102ad68c..7b346eaa30cb 100644
--- a/packages/navigation/setup.cfg
+++ b/packages/navigation/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -35,8 +34,8 @@ install_requires =
     galaxy-util
     PyYAML
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.packages.find]
 exclude =
-    tests*
\ No newline at end of file
+    tests*
diff --git a/packages/objectstore/setup.cfg b/packages/objectstore/setup.cfg
index 69fc1352966c..325319980b0f 100644
--- a/packages/objectstore/setup.cfg
+++ b/packages/objectstore/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -36,8 +35,8 @@ install_requires =
     pydantic<2
     PyYAML
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.packages.find]
 exclude =
-    tests*
\ No newline at end of file
+    tests*
diff --git a/packages/schema/setup.cfg b/packages/schema/setup.cfg
index 507f563909ea..8c9e16c46a7f 100644
--- a/packages/schema/setup.cfg
+++ b/packages/schema/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -34,7 +33,7 @@ install_requires =
     galaxy-util
     pydantic[email]
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.packages.find]
 exclude =
diff --git a/packages/selenium/setup.cfg b/packages/selenium/setup.cfg
index a95b3a449dfa..338943d2013e 100644
--- a/packages/selenium/setup.cfg
+++ b/packages/selenium/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -39,7 +38,7 @@ install_requires =
     requests
     selenium!=4.11.0,!=4.11.1,!=4.11.2
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.entry_points]
 console_scripts =
@@ -47,4 +46,4 @@ console_scripts =
 
 [options.packages.find]
 exclude =
-    tests*
\ No newline at end of file
+    tests*
diff --git a/packages/test_api/setup.cfg b/packages/test_api/setup.cfg
index 2a09121053d3..a759e952956e 100644
--- a/packages/test_api/setup.cfg
+++ b/packages/test_api/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -38,7 +37,7 @@ install_requires =
     requests
     tuspy
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.extras_require]
 driver =
@@ -46,4 +45,4 @@ driver =
 
 [options.packages.find]
 exclude =
-    tests*
\ No newline at end of file
+    tests*
diff --git a/packages/test_base/setup.cfg b/packages/test_base/setup.cfg
index a1eec0ef392e..17c957eec67a 100644
--- a/packages/test_base/setup.cfg
+++ b/packages/test_base/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -41,8 +40,8 @@ install_requires =
     PyYAML
     requests
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.packages.find]
 exclude =
-    tests*
\ No newline at end of file
+    tests*
diff --git a/packages/test_driver/setup.cfg b/packages/test_driver/setup.cfg
index cd64afd37a8c..9268dd3eb584 100644
--- a/packages/test_driver/setup.cfg
+++ b/packages/test_driver/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -43,8 +42,8 @@ install_requires =
     graphene-sqlalchemy==3.0.0b3  # these are only needed by tool shed - which we've split out but the test driver loads
     starlette-graphene3
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.packages.find]
 exclude =
-    tests*
\ No newline at end of file
+    tests*
diff --git a/packages/test_selenium/setup.cfg b/packages/test_selenium/setup.cfg
index dca41568d040..177fbc9ba8ad 100644
--- a/packages/test_selenium/setup.cfg
+++ b/packages/test_selenium/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -43,7 +42,7 @@ install_requires =
     requests
     selenium!=4.11.0,!=4.11.1,!=4.11.2
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.extras_require]
 driver =
diff --git a/packages/tool_shed/setup.cfg b/packages/tool_shed/setup.cfg
index 50b32d282a99..41d9897244a1 100644
--- a/packages/tool_shed/setup.cfg
+++ b/packages/tool_shed/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -34,7 +33,7 @@ install_requires =
     galaxy-web-stack
     galaxy-web-apps
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.packages.find]
 exclude =
diff --git a/packages/tool_util/setup.cfg b/packages/tool_util/setup.cfg
index 817bd9080842..1473adc48bbc 100644
--- a/packages/tool_util/setup.cfg
+++ b/packages/tool_util/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -43,7 +42,7 @@ install_requires =
     sortedcontainers
     typing-extensions
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.entry_points]
 console_scripts =
diff --git a/packages/tours/setup.cfg b/packages/tours/setup.cfg
index 1186d0ad6ccf..999022f00af8 100644
--- a/packages/tours/setup.cfg
+++ b/packages/tours/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -36,7 +35,7 @@ install_requires =
     pydantic<2
     PyYAML
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.entry_points]
 console_scripts =
diff --git a/packages/util/setup.cfg b/packages/util/setup.cfg
index 03afa41b853b..c634f3537459 100644
--- a/packages/util/setup.cfg
+++ b/packages/util/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -44,7 +43,7 @@ install_requires =
     typing-extensions
     zipstream-new
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.extras_require]
 jstree =
diff --git a/packages/web_apps/setup.cfg b/packages/web_apps/setup.cfg
index 17de386f4c12..398354b177d1 100644
--- a/packages/web_apps/setup.cfg
+++ b/packages/web_apps/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -70,7 +69,7 @@ install_requires =
     WebOb
     Whoosh
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.packages.find]
 exclude =
diff --git a/packages/web_framework/setup.cfg b/packages/web_framework/setup.cfg
index 99cce6a9e4c7..adceeced72ed 100644
--- a/packages/web_framework/setup.cfg
+++ b/packages/web_framework/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -44,7 +43,7 @@ install_requires =
     WebOb
 
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.packages.find]
 exclude =
diff --git a/packages/web_stack/setup.cfg b/packages/web_stack/setup.cfg
index 9d9e2022c398..6c1ff8aaee98 100644
--- a/packages/web_stack/setup.cfg
+++ b/packages/web_stack/setup.cfg
@@ -9,7 +9,6 @@ classifiers =
     Natural Language :: English
     Operating System :: POSIX
     Programming Language :: Python :: 3
-    Programming Language :: Python :: 3.7
     Programming Language :: Python :: 3.8
     Programming Language :: Python :: 3.9
     Programming Language :: Python :: 3.10
@@ -36,8 +35,8 @@ install_requires =
     galaxy-util
     SQLAlchemy>=1.4.25,<2
 packages = find:
-python_requires = >=3.7
+python_requires = >=3.8
 
 [options.packages.find]
 exclude =
-    tests*
\ No newline at end of file
+    tests*
diff --git a/pyproject.toml b/pyproject.toml
index ec98c6f49ef2..fe4a78b1a751 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -1,6 +1,6 @@
 [tool.black]
 line-length = 120
-target-version = ['py37']
+target-version = ['py38']
 include = '\.pyi?$'
 extend-exclude = '''
 ^/(
@@ -50,7 +50,7 @@ Cheetah3 = "!=3.2.6.post2"  # yanked release, https://github.com/python-poetry/p
 cloudauthz = "==0.6.0"
 cloudbridge = "*"
 circus = "*"
-cwltool = "==3.1.20221109155812"
+cwltool = ">=3.1.20221109155812"  # TODO: pin to a newer working version
 conda-package-streaming = "*"
 dictobj = "*"
 dnspython = "*"
@@ -93,9 +93,9 @@ pydantic = {version = "<2", extras = ["email"]}
 PyJWT = "*"
 pykwalify = "*"
 pylibmagic = "*"
-pyparsing = "<3.1"  # Needed by matplotlib on Python >=3.8 https://github.com/matplotlib/matplotlib/issues/26152
+pyparsing = "*"
 pysam = ">=0.21"  # for Python 3.11 support on macOS
-python = ">=3.7,<3.12"  # Keep maximum version strict to appease numpy and scipy
+python = ">=3.8,<3.12"  # Keep maximum version strict to appease numpy and scipy
 python-dateutil = "*"
 python-magic = "*"
 python-multipart = "*"  # required to support form parsing in FastAPI/Starlette
@@ -155,6 +155,7 @@ pytest-postgresql = "!=3.0.0"  # https://github.com/ClearcodeHQ/pytest-postgresq
 pytest-shard = "*"
 responses = "*"
 selenium = "*"
+urllib3 = "<2"  # Necessary for poetry for resolve deps for Python 3.8+
 seletools = "*"
 Sphinx = ">=4.0"  # support docutils 0.17
 sphinx-rtd-theme = ">=1.0.0,<2"  # https://github.com/python-poetry/poetry/issues/8194
@@ -184,7 +185,7 @@ types-six = "*"
 # Enable: pycodestyle errors (E), Pyflakes (F), flake8-bugbear (B),
 # flake8-logging-format (G) and pyupgrade (UP)
 select = ["E", "F", "B", "G", "UP"]
-target-version = "py37"
+target-version = "py38"
 # Exceptions:
 # B008 Do not perform function calls in argument defaults (for FastAPI Depends and Body)
 # B9 flake8-bugbear opinionated warnings
diff --git a/scripts/check_python.py b/scripts/check_python.py
index 99fd1c9fcbe8..62a8cfbfa59e 100644
--- a/scripts/check_python.py
+++ b/scripts/check_python.py
@@ -7,7 +7,7 @@
 
 
 def check_python():
-    if sys.version_info[:2] >= (3, 7):  # noqa: UP036
+    if sys.version_info[:2] >= (3, 8):  # noqa: UP036
         # supported
         return
     else:
@@ -15,7 +15,7 @@ def check_python():
         msg = (
             """\
 ERROR: Your Python version is: %s
-Galaxy is currently supported on Python >=3.7 .
+Galaxy is currently supported on Python >=3.8 .
 To run Galaxy, please install a supported Python version.
 If a supported version is already installed but is not your default,
 https://docs.galaxyproject.org/en/latest/admin/python.html contains instructions
diff --git a/scripts/common_startup.sh b/scripts/common_startup.sh
index 720f64a79110..e1fa46fdbac6 100755
--- a/scripts/common_startup.sh
+++ b/scripts/common_startup.sh
@@ -14,7 +14,7 @@ SKIP_NODE=${GALAXY_SKIP_NODE:-0}
 INSTALL_PREBUILT_CLIENT=${GALAXY_INSTALL_PREBUILT_CLIENT:-0}
 NODE_VERSION=${GALAXY_NODE_VERSION:-"$(cat client/.node_version)"}
 : "${YARN_INSTALL_OPTS:=--network-timeout 300000 --check-files}"
-: "${GALAXY_CONDA_PYTHON_VERSION:=3.7}"
+: "${GALAXY_CONDA_PYTHON_VERSION:=3.8}"
 
 for arg in "$@"; do
     if [ "$arg" = "--skip-venv" ]; then
@@ -41,7 +41,7 @@ RMFILES="
     lib/pkg_resources.pyc
 "
 
-MIN_PYTHON_VERSION=3.7
+MIN_PYTHON_VERSION=3.8
 MIN_PIP_VERSION=20.3
 
 # return true if $1 is in $2 else false
diff --git a/test/integration/test_celery_user_rate_limit.py b/test/integration/test_celery_user_rate_limit.py
index 5d58d02f9025..ac69062dfda1 100644
--- a/test/integration/test_celery_user_rate_limit.py
+++ b/test/integration/test_celery_user_rate_limit.py
@@ -24,7 +24,7 @@ def mock_user_id_task(task_user_id: int):
     return task_user_id
 
 
-@lru_cache()
+@lru_cache
 def sqlite_url():
     path = tempfile.NamedTemporaryFile().name
     dburl = f"sqlite:///{path}"
@@ -32,7 +32,7 @@ def sqlite_url():
     return dburl
 
 
-@lru_cache()
+@lru_cache
 def setup_users(dburl: str, num_users: int = 2):
     """
     Setup test users in galaxy_user table with user id's starting from 2.
diff --git a/test/integration/test_kubernetes_runner.py b/test/integration/test_kubernetes_runner.py
index 62a353693ea5..728d2a4fbfdd 100644
--- a/test/integration/test_kubernetes_runner.py
+++ b/test/integration/test_kubernetes_runner.py
@@ -4,6 +4,7 @@
 import collections
 import json
 import os
+import shlex
 import string
 import subprocess
 import tempfile
@@ -18,10 +19,7 @@
 from typing_extensions import Literal
 
 from galaxy.tool_util.verify.wait import timeout_type
-from galaxy.util import (
-    shlex_join,
-    unicodify,
-)
+from galaxy.util import unicodify
 from galaxy_test.base.populators import (
     DatasetPopulator,
     DEFAULT_TIMEOUT,
@@ -361,7 +359,7 @@ def get_kubectl_logs(allow_wait: bool = True) -> Optional[str]:
                 if allow_wait and "is waiting to start" in p.stderr:
                     return None
                 raise Exception(
-                    f"Command '{shlex_join(log_cmd)}' failed with exit code: {p.returncode}.\nstdout: {p.stdout}\nstderr: {p.stderr}"
+                    f"Command '{shlex.join(log_cmd)}' failed with exit code: {p.returncode}.\nstdout: {p.stdout}\nstderr: {p.stderr}"
                 )
             return p.stdout
 
diff --git a/test/unit/app/managers/test_NotificationManager.py b/test/unit/app/managers/test_NotificationManager.py
index 99d493b3ef3b..ad707619d4b1 100644
--- a/test/unit/app/managers/test_NotificationManager.py
+++ b/test/unit/app/managers/test_NotificationManager.py
@@ -276,7 +276,7 @@ def test_update_user_notifications(self):
         assert user_notification.seen_time is None
         assert user_notification.deleted is False
         request = UserNotificationUpdateRequest(seen=True)
-        self.notification_manager.update_user_notifications(user, set([notification.id]), request)
+        self.notification_manager.update_user_notifications(user, {notification.id}, request)
         user_notification = self.notification_manager.get_user_notification(user, notification.id)
         assert user_notification.seen_time is not None
         assert user_notification.deleted is False
diff --git a/tox.ini b/tox.ini
index 2bde302555f9..c68144f12885 100644
--- a/tox.ini
+++ b/tox.ini
@@ -39,6 +39,8 @@ passenv =
     GALAXY_CONFIG_OVERRIDE_DATABASE_CONNECTION
     APP_WEBSERVER
     TERM
+    TEST_PYTHON
+    TZ
 setenv =
     coverage: GALAXY_TEST_COVERAGE=1
     first_startup: GALAXY_CONFIG_DATABASE_AUTO_MIGRATE=true