diff --git a/lib/galaxy/schema/configuration.py b/lib/galaxy/schema/configuration.py
index 414a6e834497..93e910ab02fa 100644
--- a/lib/galaxy/schema/configuration.py
+++ b/lib/galaxy/schema/configuration.py
@@ -1977,16 +1977,6 @@ class FullGalaxyConfig(AdminExposableGalaxyConfig, SchemaCompatibleConfigValues)
         ),
     ] = "tool_search_index"
 
-    delay_tool_initialization: Annotated[
-        bool,
-        Field(
-            title="Delay Tool Initialization",
-            description="""Set this to true to delay parsing of tool inputs and outputs until they are needed.
-This results in faster startup times but uses more memory when using forked Galaxy processes.
-""",
-        ),
-    ] = False
-
     biotools_content_directory: Annotated[
         Optional[str],
         Field(