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test.sh
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test.sh
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########### Test Suite for Goalign command line tools ##############
set -e
set -u
set -o pipefail
TESTDATA="tests/data"
GOALIGN=./goalign
echo "->goalign addid"
cat > expected <<EOF
>prefix_Seq0000_suffix
GATTAATTTGCCGTAGGCCAGAATCTGAAGATCGAACACTTTAAGTTTTCACTTCTAATGGAGAGGACTAGTTCATACTT
TTTAAACACTTTTACATCGA
>prefix_Seq0001_suffix
TGTCGGACCTAAGTATTGAGTACAACGGTGTATTCCAGCGGTGGAGAGGTCTATTTTTCCGGTTGAAGGACTCTAGAGCT
GTAAAGGGTATGGCCATGTG
>prefix_Seq0002_suffix
CTAAGCGCGGGCGGATTGCTGTTGGAGCAAGGTTAAATACTCGGCAATGCCCCATGATCCCCCAAGGACAATAAGAGCGA
AGTTAGAACAAATGAACCCC
>prefix_Seq0003_suffix
GAGTGGAGGCTTTATGGCACAAGGTATTAGAGACTGAGGGGCACCCCGGCATGGTAAGCAGGAGCCATCGCGAAGGCTTC
AGGTATCTTCCTGTGTTACC
>prefix_Seq0004_suffix
CATAGCCCCTGATGCCCTGACCCGTGTCGCGGCAACGTCTACATTTCACGATAAATACTCCGCTGCTAGTCGGCTCTAGA
TGCTTTTCTTCCAGATCTGG
EOF
${GOALIGN} random --seed 10 -n 5 | ${GOALIGN} addid -n prefix_ | ${GOALIGN} addid -n _suffix -r > result
diff -q -b result expected
rm -f expected result mapfile
echo "->goalign addid unaligned"
cat > input <<EOF
>Seq0000
GATTAATTTGCCGTAGGCCAAAGATCGAACACTTTAAGTTTTCACTTCTAATGGAGAGGACTAGTTCATACTT
TTTAAACACTTTTACATCGA
>Seq0001
TGTCGGACCTAAGTATTGAGGGTCTATTTTTCCGGTTGAAGGACTCTAGAGCT
GTAAAGGGTATGGCCATGTG
>Seq0002
CTAAGCGCGGGCGGATTGCTGTTGGAGCAAGGTTAAATACTCGGCAATGCCCCATGATCCCCCAAGGACAATAAGAGCGA
>Seq0003
AGGTATCTTCCTGTGTTACC
>Seq0004
CA
EOF
cat > expected <<EOF
>prefix_Seq0000_suffix
GATTAATTTGCCGTAGGCCAAAGATCGAACACTTTAAGTTTTCACTTCTAATGGAGAGGACTAGTTCATACTTTTTAAAC
ACTTTTACATCGA
>prefix_Seq0001_suffix
TGTCGGACCTAAGTATTGAGGGTCTATTTTTCCGGTTGAAGGACTCTAGAGCTGTAAAGGGTATGGCCATGTG
>prefix_Seq0002_suffix
CTAAGCGCGGGCGGATTGCTGTTGGAGCAAGGTTAAATACTCGGCAATGCCCCATGATCCCCCAAGGACAATAAGAGCGA
>prefix_Seq0003_suffix
AGGTATCTTCCTGTGTTACC
>prefix_Seq0004_suffix
CA
EOF
${GOALIGN} addid -n prefix_ -i input --unaligned | ${GOALIGN} addid -n _suffix -r --unaligned > result
diff -q -b result expected
rm -f expected result mapfile input
echo "->goalign clean sites"
cat > input <<EOF
>Seq0000
GATTAATTT-CCGTAGGCCAGAA-CTGAA---CGAA-ACTTTAAGTTTTCACTT-TAATGGAGAGGACTAGTTCATACTT
-TT-A-CACTTTTACATCGA
>Seq0001
-GTCGGACCTAAG-ATTGAGTACAACGGTG-ATTCCAGCGG-GGAGAGGTCT-TTTTTCCGG-TGAAGGACTCTAG-GCT
GTAAA-G-TATGGC-ATGTG
>Seq0002
CTAA-CGCGGGCGGATT-CTGT-GGA-CAAGGTTAAATAC-CGGCAATGC-CCATGATCCCCCA-GGAC-ATAAGAGCGA
AGTT--AACAAATGAACCCC
>Seq0003
GAGT-GA-GCTTTATGGCA-AAGGTATTAG-GACTGA----CACCCCGGCATGGTAAGCAGGAGCCA-CGCGAAGGCTTC
AGGTATCTTCCTGT-TTACC
>Seq0004
CATAGCCCC--ATG-CC-GACC-GTGTCGCGGCAACGTCTACA-TTCACGATAAA-ACTCCG-TGCTAGTCGGCTC-AGA
TGCTTTTCTTCCA-ATCTGG
>Seq0005
-GTTTGA-TAT---CGCCGGCTTAGTGCTGACA-TGATGCTCCGTT-TAAGGGTCCTGATGTT-TTGTGCTC-CGCATAT
TA-AGCTGAGTTTCCCAAAG
>Seq0006
TCGC-ACGG-GTGGAATGTACGTTAT-GCA-TAATCAGCG-CTTTC-CCGACATGCCCCCTCCGTGGCTCCTT-CG-CCA
-CGGCGGACCTGCGGT-TCG
>Seq0007
CT-G-AAT-CCTGCGCTATTTCGTCAGTTCGTG-ACGGGTAAC-A--GCGGTTAATGCTT-TTCCGATCAGCTCA-ACCC
ATGAAGGTGGCTCTGG-GCC
>Seq0008
TCGTTAACCCACTCTAACCACCTC-TG--GCGACATCGG-T-CTCGGCTTGGATACCTTC-TC-TATTGGACCCCAGG-C
TCAA-CTCGTGAGCTCTCT-
>Seq0009
ACCT-CGGCTCTAGACAGCTGAA--CCGGTTCCGAGCACTGTA-GGAAACTTG-AAAGGCTCGACGGA-G-TTGTTCC-C
AGAGTGGGA-TATAACATA-
EOF
cat > expected <<EOF
>Seq0000
ATATGGCGATCAAAGTTCCAATGAGATACTTCCTTTACG
>Seq0001
GCGATTATACTCCGAGGTTTTCCGAATAGTTGTTGGAGT
>Seq0002
TACGTTCGTATAAGCTGCCATCCCGGTGCAGACAATACC
>Seq0003
ATGAGGAAAAATGACGGCTAGCAGCAGGTCGCTCTGTAC
>Seq0004
AACCCCGCCGCACATACGTACTCGCTGTAAGTTCCATTG
>Seq0005
GTGAGCGCTTATGCTTAAGTGATTTGCGTTATATTTCAA
>Seq0006
CCACATTCGTATCTTCCGCCCCCCGGTCCACGCTGCTTC
>Seq0007
TGAACTTTCAGACCAGCGTGCTTTGACACCTGGCTCGGC
>Seq0008
CTAAAACCCTAATCCCTTGCTTCTATCCGCCTGGAGCCT
>Seq0009
CTCGCACGACCAGAGAACTAGGCCGGTTCCGGATATCTA
EOF
cat > expectedlog <<EOF
Alignment (0) length before cleaning=100
Alignment (0) length after cleaning=39
Alignment (0) number of gaps=61
Alignment (0) number of start gaps=1
Alignment (0) number of end gaps=1
EOF
cat > expectedlog2 <<EOF
Alignment (0) length before cleaning=100
Alignment (0) length after cleaning=39
Alignment (0) number of n=61
Alignment (0) number of start n=1
Alignment (0) number of end n=1
EOF
${GOALIGN} clean sites -i input > result 2>log
diff -q -b result expected
diff -q -b log expectedlog
rm -f result mapfile log expectedlog
${GOALIGN} replace -s - -n N -i input | ${GOALIGN} clean sites --char n --ignore-case > result 2>log
diff -q -b result expected
diff -q -b log expectedlog2
rm -f expected result mapfile log expectedlog2
echo "->goalign clean sites --ends"
cat > input <<EOF
>Seq0000
--GGTCCACTCTTTTGTCTT-TACCTA-G-
>Seq0001
G---CACCGGC-CGTAATGACG-ACCC--T
>Seq0002
-T-G-TTTCCTGC-AACAT-ACC-AAC-C-
>Seq0003
A-ACCACAACAGTCA-GTACTCTT-TG--T
>Seq0004
-----GAAGG-CCAAGGT-TCGCCGCCC--
EOF
cat > expected <<EOF
>Seq0000
GTCCACTCTTTTGTCTT-TACCTA
>Seq0001
-CACCGGC-CGTAATGACG-ACCC
>Seq0002
G-TTTCCTGC-AACAT-ACC-AAC
>Seq0003
CCACAACAGTCA-GTACTCTT-TG
>Seq0004
--GAAGG-CCAAGGT-TCGCCGCC
EOF
cat > expectedlog <<EOF
Alignment (0) length before cleaning=30
Alignment (0) length after cleaning=24
Alignment (0) number of gaps=6
Alignment (0) number of start gaps=3
Alignment (0) number of end gaps=3
EOF
cat > expectedpos <<EOF
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
EOF
cat > expectedrmpos <<EOF
0
1
2
27
28
29
EOF
${GOALIGN} clean sites -i input -c 0.5 --positions pos --positions-rm rmpos > result 2>log
diff -q -b result expected
diff -q -b log expectedlog
diff -q -b pos expectedpos
diff -q -b rmpos expectedrmpos
rm -f expected result log expectedlog expectedpos expectedrmpos pos rmpos
echo "->goalign clean sites --char ACG --reverse"
cat > input <<EOF
>A
N-ANGA-GACC
>B
N-TN-T-TTTC
>C
NCTN-TTT--T
>D
N-ANCCCCCCC
EOF
cat > expected <<EOF
>A
-AGA-GACC
>B
-T-T-TTTC
>C
CT-TTT--T
>D
-ACCCCCCC
EOF
cat > expectedlog <<EOF
Alignment (0) length before cleaning=11
Alignment (0) length after cleaning=9
Alignment (0) number of ACG=2
Alignment (0) number of start ACG=1
Alignment (0) number of end ACG=0
EOF
cat > expectedpos <<EOF
1
2
4
5
6
7
8
9
10
EOF
cat > expectedrmpos <<EOF
0
3
EOF
${GOALIGN} clean sites --char ACG --reverse -c 0.8 --positions pos --positions-rm rmpos -i input > result 2>log
diff -q -b result expected
diff -q -b log expectedlog
diff -q -b pos expectedpos
diff -q -b rmpos expectedrmpos
rm -f input expected result log expectedlog pos expectedpos rmpos expectedrmpos
echo "->goalign clean sites --ends --char MAJ"
cat > input <<EOF
>A
N-ANGA-GACC
>B
N-TN-T-TTTC
>C
NCTN-TTT--T
>D
N-ANCCCCCCC
EOF
cat > expected <<EOF
>A
AGA-GAC
>B
T-T-TTT
>C
T-TTT--
>D
ACCCCCC
EOF
cat > expected2 <<EOF
>A
ANGA-GAC
>B
TN-T-TTT
>C
TN-TTT--
>D
ANCCCCCC
EOF
cat > expected3 <<EOF
>A
-AGA-GACC
>B
-T-T-TTTC
>C
CT-TTT--T
>D
-ACCCCCCC
EOF
cat > expectedlog <<EOF
Alignment (0) length before cleaning=11
Alignment (0) length after cleaning=7
Alignment (0) number of maj=4
Alignment (0) number of start maj=2
Alignment (0) number of end maj=1
EOF
cat > expectedlog2 <<EOF
Alignment (0) length before cleaning=11
Alignment (0) length after cleaning=8
Alignment (0) number of maj=3
Alignment (0) number of start maj=2
Alignment (0) number of end maj=1
EOF
cat > expectedlog3 <<EOF
Alignment (0) length before cleaning=11
Alignment (0) length after cleaning=9
Alignment (0) number of maj=2
Alignment (0) number of start maj=1
Alignment (0) number of end maj=0
EOF
cat > expectedpos <<EOF
2
4
5
6
7
8
9
EOF
cat > expectedrmpos <<EOF
0
1
3
10
EOF
${GOALIGN} clean sites -i input -c 0.6 --char MAJ --positions pos --positions-rm rmpos > result 2>log
diff -q -b result expected
diff -q -b log expectedlog
diff -q -b pos expectedpos
diff -q -b rmpos expectedrmpos
rm -f expected result log expectedlog pos expectedpos rmpos expectedrmpos
${GOALIGN} clean sites -i input -c 0.6 --char MAJ --ends > result 2>log
diff -q -b result expected2
diff -q -b log expectedlog2
rm -f expected2 result log expectedlog2
${GOALIGN} clean sites -i input -c 1 --char MAJ > result 2>log
diff -q -b result expected3
diff -q -b log expectedlog3
rm -f expected3 result log expectedlog3
echo "->goalign clean seqs"
cat > input <<EOF
>Seq0000
GATTAATTTGCCGTAGGCCAGAATCTGAAGATCGAACACTTTAAGTTTTCACTTCTAATGGAGAGGACTAGTTCATACTT
TTTAAACACTTTTACATCGA
>Seq0001
--T-------A------G---A-AA-G--------C---G------A-G--T-T---T-C-G--GA---AC----G---T
-T-A-------G---ATGTG
>Seq0002
-T-----C-GG---A-T---G------CAAGG-T-A---------A--GC--C---A-C---C-----C-----G---G-
AG-------A--TG----C-
>Seq0003
GAGTGGAGGCTTTATGGCACAAGGTATTAGAGACTGAGGGGCACCCCGGCATGGTAAGCAGGAGCCATCGCGAAGGCTTC
AGGTATCTTCCTGTGTTACC
>Seq0004
--T--C-----AT--C-------G------G---A-G--TAC--T--AC-A-----AC---G--G--A--CG-CT-----
T---T---T------T-T--
>Seq0005
AGTTTGACTATGAGCGCCGGCTTAGTGCTGACAGTGATGCTCCGTTGTAAGGGTCCTGATGTTCTTGTGCTCGCGCATAT
TAGAGCTGAGTTTCCCAAAG
>Seq0006
T----A----G----A-GT-CG-------A--AA-CAG-G-----C--C---A-------T-------T-CT--C--C-A
---G----C----G-T--C-
>Seq0007
CTGGTAATACCTGCGCTATTTCGTCAGTTCGTGTACGGGTAACGATAGCGGTTAATGCTTATTCCGATCAGCTCACACCC
ATGAAGGTGGCTCTGGAGCC
>Seq0008
T---T--CCCA--------A-CT--------G--A---G---C-C----TGG-----T---T-------G-C-C--G---
-C------GT-A-CT-T---
>Seq0009
ACCTACGGCTCTAGACAGCTGAAGTCCGGTTCCGAGCACTGTACGGAAACTTGAAAAGGCTCGACGGAGGCTTGTTCCGC
AGAGTGGGACTATAACATAC
EOF
cat > expected <<EOF
>Seq0000
GATTAATTTGCCGTAGGCCAGAATCTGAAGATCGAACACTTTAAGTTTTCACTTCTAATGGAGAGGACTAGTTCATACTT
TTTAAACACTTTTACATCGA
>Seq0003
GAGTGGAGGCTTTATGGCACAAGGTATTAGAGACTGAGGGGCACCCCGGCATGGTAAGCAGGAGCCATCGCGAAGGCTTC
AGGTATCTTCCTGTGTTACC
>Seq0005
AGTTTGACTATGAGCGCCGGCTTAGTGCTGACAGTGATGCTCCGTTGTAAGGGTCCTGATGTTCTTGTGCTCGCGCATAT
TAGAGCTGAGTTTCCCAAAG
>Seq0007
CTGGTAATACCTGCGCTATTTCGTCAGTTCGTGTACGGGTAACGATAGCGGTTAATGCTTATTCCGATCAGCTCACACCC
ATGAAGGTGGCTCTGGAGCC
>Seq0009
ACCTACGGCTCTAGACAGCTGAAGTCCGGTTCCGAGCACTGTACGGAAACTTGAAAAGGCTCGACGGAGGCTTGTTCCGC
AGAGTGGGACTATAACATAC
EOF
cat > expectedlog <<EOF
[Warning] in cmd/cleanseqs.go (line 61), message: Alignment (0) #seqs before cleaning=10
[Warning] in cmd/cleanseqs.go (line 62), message: Alignment (0) #seqs after cleaning=5
[Warning] in cmd/cleanseqs.go (line 63), message: Alignment (0) removed sequences=5
EOF
${GOALIGN} clean seqs -i input > result 2>log
diff -q -b result expected
rm -f result mapfile log expectedlog
${GOALIGN} clean seqs --char GAP -i input > result 2>log
diff -q -b result expected
rm -f result mapfile log expectedlog
${GOALIGN} replace -s "-" -n "N" -i input | ${GOALIGN} clean seqs --char n --ignore-case > result 2>log
diff -q -b result expected
rm -f expected result mapfile log expectedlog
echo "->goalign clean seqs --ignore-gaps"
cat > input <<EOF
>Seq0000
GATTAATTTGCCGTAGGCCAGAATCTGAAGATCGAACACTTTAAGTTTTCACTTCTAATGGAGAGGACTAGTTCATACTT
TTTAAACACTTTTACATCGA
>Seq0001
--T-------A------G---A-AA-GNNNNNNNNC---G------A-G--T-T---T-C-G--GA---AC----G---T
-T-A-------G---ATGTG
>Seq0002
-T-----C-GG---A-T---G------CAAGG-T-A---------A--GC--C---A-C---C-----C-----G---G-
AG-------A--TG----C-
>Seq0003
GAGTGGAGGCTTTATGGCACAAGGTATTAGAGACTGAGGGGCACCCCGGCATGGTAAGCAGGAGCCATCGCGAAGGCTTC
AGGTATCTTCCTGTGTTACC
>Seq0004
--T--C-----AT--C-------G------G---A-G--TAC--T--AC-A-----AC---G--G--A--CG-CT-----
T---T---T------T-T--
>Seq0005
AGTTTGACTATGAGCGCCGGCTTAGTGCTGACAGTGATGCTCCGTTGTAAGGGTCCTGATGTTCTTGTGCTCGCGCATAT
TAGAGCTGAGTTTCCCAAAG
>Seq0006
T----A----G----A-GT-CG-------A--AA-CAG-G-----C--C---A-------T-------T-CT--C--C-A
---G----C----G-T--C-
>Seq0007
CTGGTAATACCTGCGCTATTTCGTCAGTTCGTGTACGGGTAACGATAGCGGTTAATGCTTATTCCGATCAGCTCACACCC
ATGAAGGTGGCTCTGGAGCC
>Seq0008
T---T--CCCA--------A-CT--------G--A---G---C-C----TGG-----T---T-------G-C-C--G---
-C------GT-A-CT-T---
>Seq0009
ACCTACGGCTCTAGACAGCTGAAGTCCGGTTCCGAGCACTGTACGGAAACTTGAAAAGGCTCGACGGAGGCTTGTTCCGC
AGAGTGGGACTATAACATAC
EOF
cat > expected <<EOF
>Seq0000
GATTAATTTGCCGTAGGCCAGAATCTGAAGATCGAACACTTTAAGTTTTCACTTCTAATGGAGAGGACTAGTTCATACTT
TTTAAACACTTTTACATCGA
>Seq0002
-T-----C-GG---A-T---G------CAAGG-T-A---------A--GC--C---A-C---C-----C-----G---G-
AG-------A--TG----C-
>Seq0003
GAGTGGAGGCTTTATGGCACAAGGTATTAGAGACTGAGGGGCACCCCGGCATGGTAAGCAGGAGCCATCGCGAAGGCTTC
AGGTATCTTCCTGTGTTACC
>Seq0004
--T--C-----AT--C-------G------G---A-G--TAC--T--AC-A-----AC---G--G--A--CG-CT-----
T---T---T------T-T--
>Seq0005
AGTTTGACTATGAGCGCCGGCTTAGTGCTGACAGTGATGCTCCGTTGTAAGGGTCCTGATGTTCTTGTGCTCGCGCATAT
TAGAGCTGAGTTTCCCAAAG
>Seq0006
T----A----G----A-GT-CG-------A--AA-CAG-G-----C--C---A-------T-------T-CT--C--C-A
---G----C----G-T--C-
>Seq0007
CTGGTAATACCTGCGCTATTTCGTCAGTTCGTGTACGGGTAACGATAGCGGTTAATGCTTATTCCGATCAGCTCACACCC
ATGAAGGTGGCTCTGGAGCC
>Seq0008
T---T--CCCA--------A-CT--------G--A---G---C-C----TGG-----T---T-------G-C-C--G---
-C------GT-A-CT-T---
>Seq0009
ACCTACGGCTCTAGACAGCTGAAGTCCGGTTCCGAGCACTGTACGGAAACTTGAAAAGGCTCGACGGAGGCTTGTTCCGC
AGAGTGGGACTATAACATAC
EOF
cat > expectedlog <<EOF
[Warning] in cmd/cleanseqs.go (line 61), message: Alignment (0) #seqs before cleaning=10
[Warning] in cmd/cleanseqs.go (line 62), message: Alignment (0) #seqs after cleaning=9
[Warning] in cmd/cleanseqs.go (line 63), message: Alignment (0) removed sequences=1
EOF
${GOALIGN} clean seqs --char N --ignore-gaps -c 0.2 -i input > result 2>log
diff -q -b result expected
diff -q -b log expectedlog
rm -f result mapfile log expectedlog
echo "->goalign clean seqs --ignore-n"
cat > input <<EOF
>Seq0000
GATTAATTTGCCGTAGGCCAGAATCTGAAGATCGAACACTTTAAGTTTTCACTTCTAATGGAGAGGACTAGTTCATACTT
TTTAAACACTTTTACATCGA
>Seq0001
NNTNNNNNNNANNNNNNGNNNANAANG--------CNNNGNNNNNNANGNNTNTNNNTNCNGNNGANNNACNNNNGNNNT
NTNANNNNNNNGNNNATGTG
>Seq0002
NTNNNNNCNGGNNNANTNNNGNNNNNNCAAGGNTNANNNNNNNNNANNGCNNCNNNANCNNNCNNNNNCNNNNNGNNNGN
AGNNNNNNNANNTGNNNNCN
>Seq0003
GAGTGGAGGCTTTATGGCACAAGGTATTAGAGACTGAGGGGCACCCCGGCATGGTAAGCAGGAGCCATCGCGAAGGCTTC
AGGTATCTTCCTGTGTTACC
>Seq0004
NNTNNCNNNNNATNNCNNNNNNNGNNNNNNGNNNANGNNTACNNTNNACNANNNNNACNNNGNNGNNANNCGNCTNNNNN
TNNNTNNNTNNNNNNTNTNN
>Seq0005
AGTTTGACTATGAGCGCCGGCTTAGTGCTGACAGTGATGCTCCGTTGTAAGGGTCCTGATGTTCTTGTGCTCGCGCATAT
TAGAGCTGAGTTTCCCAAAG
>Seq0006
TNNNNANNNNGNNNNANGTNCGNNNNNNNANNAANCAGNGNNNNNCNNCNNNANNNNNNNTNNNNNNNTNCTNNCNNCNA
NNNGNNNNCNNNNGNTNNCN
>Seq0007
CTGGTAATACCTGCGCTATTTCGTCAGTTCGTGTACGGGTAACGATAGCGGTTAATGCTTATTCCGATCAGCTCACACCC
ATGAAGGTGGCTCTGGAGCC
>Seq0008
TNNNTNNCCCANNNNNNNNANCTNNNNNNNNGNNANNNGNNNCNCNNNNTGGNNNNNTNNNTNNNNNNNGNCNCNNGNNN
NCNNNNNNGTNANCTNTNNN
>Seq0009
ACCTACGGCTCTAGACAGCTGAAGTCCGGTTCCGAGCACTGTACGGAAACTTGAAAAGGCTCGACGGAGGCTTGTTCCGC
AGAGTGGGACTATAACATAC
EOF
cat > expected <<EOF
>Seq0000
GATTAATTTGCCGTAGGCCAGAATCTGAAGATCGAACACTTTAAGTTTTCACTTCTAATGGAGAGGACTAGTTCATACTT
TTTAAACACTTTTACATCGA
>Seq0002
NTNNNNNCNGGNNNANTNNNGNNNNNNCAAGGNTNANNNNNNNNNANNGCNNCNNNANCNNNCNNNNNCNNNNNGNNNGN
AGNNNNNNNANNTGNNNNCN
>Seq0003
GAGTGGAGGCTTTATGGCACAAGGTATTAGAGACTGAGGGGCACCCCGGCATGGTAAGCAGGAGCCATCGCGAAGGCTTC
AGGTATCTTCCTGTGTTACC
>Seq0004
NNTNNCNNNNNATNNCNNNNNNNGNNNNNNGNNNANGNNTACNNTNNACNANNNNNACNNNGNNGNNANNCGNCTNNNNN
TNNNTNNNTNNNNNNTNTNN
>Seq0005
AGTTTGACTATGAGCGCCGGCTTAGTGCTGACAGTGATGCTCCGTTGTAAGGGTCCTGATGTTCTTGTGCTCGCGCATAT
TAGAGCTGAGTTTCCCAAAG
>Seq0006
TNNNNANNNNGNNNNANGTNCGNNNNNNNANNAANCAGNGNNNNNCNNCNNNANNNNNNNTNNNNNNNTNCTNNCNNCNA
NNNGNNNNCNNNNGNTNNCN
>Seq0007
CTGGTAATACCTGCGCTATTTCGTCAGTTCGTGTACGGGTAACGATAGCGGTTAATGCTTATTCCGATCAGCTCACACCC
ATGAAGGTGGCTCTGGAGCC
>Seq0008
TNNNTNNCCCANNNNNNNNANCTNNNNNNNNGNNANNNGNNNCNCNNNNTGGNNNNNTNNNTNNNNNNNGNCNCNNGNNN
NCNNNNNNGTNANCTNTNNN
>Seq0009
ACCTACGGCTCTAGACAGCTGAAGTCCGGTTCCGAGCACTGTACGGAAACTTGAAAAGGCTCGACGGAGGCTTGTTCCGC
AGAGTGGGACTATAACATAC
EOF
cat > expectedlog <<EOF
[Warning] in cmd/cleanseqs.go (line 61), message: Alignment (0) #seqs before cleaning=10
[Warning] in cmd/cleanseqs.go (line 62), message: Alignment (0) #seqs after cleaning=9
[Warning] in cmd/cleanseqs.go (line 63), message: Alignment (0) removed sequences=1
EOF
${GOALIGN} clean seqs --char GAP --ignore-n -c 0.2 -i input > result 2>log
diff -q -b result expected
diff -q -b log expectedlog
rm -f result mapfile expected log expectedlog
echo "->goalign clean seqs --ignore-n /2"
cat > input <<EOF
>Seq0000
ACGTN
>Seq0001
AANNn
>Seq0002
ACGT-
>Seq0003
ACGT-
EOF
cat > expected <<EOF
>Seq0000
ACGTN
>Seq0002
ACGT-
>Seq0003
ACGT-
EOF
cat > expectedlog <<EOF
[Warning] in cmd/cleanseqs.go (line 61), message: Alignment (0) #seqs before cleaning=4
[Warning] in cmd/cleanseqs.go (line 62), message: Alignment (0) #seqs after cleaning=3
[Warning] in cmd/cleanseqs.go (line 63), message: Alignment (0) removed sequences=1
EOF
${GOALIGN} clean seqs --char A --ignore-n -c 0.5 -i input > result 2>log
diff -q -b result expected
diff -q -b log expectedlog
rm -f result mapfile expected log expectedlog
echo "->goalign clean seqs --ignore-n /3"
cat > input <<EOF
>Seq0000
ACGTN
>Seq0001
AaNnN
>Seq0002
ACGT-
>Seq0003
ACGT-
EOF
cat > expected <<EOF
>Seq0000
ACGTN
>Seq0002
ACGT-
>Seq0003
ACGT-
EOF
cat > expectedlog <<EOF
[Warning] in cmd/cleanseqs.go (line 61), message: Alignment (0) #seqs before cleaning=4
[Warning] in cmd/cleanseqs.go (line 62), message: Alignment (0) #seqs after cleaning=3
[Warning] in cmd/cleanseqs.go (line 63), message: Alignment (0) removed sequences=1
EOF
${GOALIGN} clean seqs --char A --ignore-n --ignore-case -c 0.9 -i input > result 2>log
diff -q -b result expected
diff -q -b log expectedlog
rm -f result mapfile expected log expectedlog
echo "->goalign random"
cat > expected <<EOF
>Seq0000
GATTAATTTGCCGTAGGCCA
>Seq0001
GAATCTGAAGATCGAACACT
>Seq0002
TTAAGTTTTCACTTCTAATG
>Seq0003
GAGAGGACTAGTTCATACTT
>Seq0004
TTTAAACACTTTTACATCGA
>Seq0005
TGTCGGACCTAAGTATTGAG
>Seq0006
TACAACGGTGTATTCCAGCG
>Seq0007
GTGGAGAGGTCTATTTTTCC
>Seq0008
GGTTGAAGGACTCTAGAGCT
>Seq0009
GTAAAGGGTATGGCCATGTG
EOF
${GOALIGN} random --seed 10 -l 20 > result
diff -q -b result expected
rm -f expected result mapfile
echo "->goalign reformat fasta"
cat > expected <<EOF
>Seq0000
GATTA
>Seq0001
ATTTG
>Seq0002
CCGTA
>Seq0003
GGCCA
>Seq0004
GAATC
EOF
${GOALIGN} random -n 5 -l 5 --seed 10 -p | ${GOALIGN} reformat fasta -p > result
diff -q -b result expected
rm -f expected result mapfile
echo "->goalign reformat fasta strict"
cat > expected <<EOF
>Seq0000
GATTA
>Seq0001
ATTTG
>Seq0002
CCGTA
>Seq0003
GGCCA
>Seq0004
GAATC
EOF
${GOALIGN} random -n 5 -l 5 --seed 10 -p --input-strict | ${GOALIGN} reformat fasta -p > result
diff -q -b result expected
rm -f expected result mapfile
echo "->goalign reformat fasta names with spaces"
cat > input <<EOF
> Seq0000
GATTA
> Seq0001
ATTTG
> Seq0002
CCGTA
> Seq0003
GGCCA
> Seq0004
GAATC
EOF
cat > expected <<EOF
>Seq0000
GATTA
>Seq0001
ATTTG
>Seq0002
CCGTA
>Seq0003
GGCCA
>Seq0004
GAATC
EOF
${GOALIGN} reformat fasta -i input -o result
diff -q -b result expected
rm -f expected input result
echo "->goalign reformat fasta sequences with spaces"
cat > input <<EOF
> Seq0000
GA T TA
> Seq0001
A TT TG
> Seq0002
CC G TA
> Seq0003
GG CC A
> Seq0004
G AA T C
EOF
cat > expected <<EOF
>Seq0000
GATTA
>Seq0001
ATTTG
>Seq0002
CCGTA
>Seq0003
GGCCA
>Seq0004
GAATC
EOF
${GOALIGN} reformat fasta -i input -o result
diff -q -b result expected
rm -f expected input result
echo "->goalign reformat phylip"
cat > expected <<EOF
5 75
Seq0000 GATTAATTTG CCGTAGGCCA GAATCTGAAG ATCGAACACT TTAAGTTTTC ACTTCTAATG
Seq0001 TACTTTTTAA ACACTTTTAC ATCGATGTCG GACCTAAGTA TTGAGTACAA CGGTGTATTC
Seq0002 CTATTTTTCC GGTTGAAGGA CTCTAGAGCT GTAAAGGGTA TGGCCATGTG CTAAGCGCGG
Seq0003 AGCAAGGTTA AATACTCGGC AATGCCCCAT GATCCCCCAA GGACAATAAG AGCGAAGTTA
Seq0004 GAGTGGAGGC TTTATGGCAC AAGGTATTAG AGACTGAGGG GCACCCCGGC ATGGTAAGCA
GAGAGGACTA GTTCA
CAGCGGTGGA GAGGT
GCGGATTGCT GTTGG
GAACAAATGA ACCCC
GGAGCCATCG CGAAG
EOF
${GOALIGN} random -n 5 -l 75 --seed 10 | ${GOALIGN} reformat phylip > result
diff -q -b result expected
rm -f expected result mapfile
echo "->goalign reformat phylip --one-line"
cat > expected <<EOF
5 75
Seq0000 GATTAATTTG CCGTAGGCCA GAATCTGAAG ATCGAACACT TTAAGTTTTC ACTTCTAATG GAGAGGACTA GTTCA
Seq0001 TACTTTTTAA ACACTTTTAC ATCGATGTCG GACCTAAGTA TTGAGTACAA CGGTGTATTC CAGCGGTGGA GAGGT
Seq0002 CTATTTTTCC GGTTGAAGGA CTCTAGAGCT GTAAAGGGTA TGGCCATGTG CTAAGCGCGG GCGGATTGCT GTTGG
Seq0003 AGCAAGGTTA AATACTCGGC AATGCCCCAT GATCCCCCAA GGACAATAAG AGCGAAGTTA GAACAAATGA ACCCC
Seq0004 GAGTGGAGGC TTTATGGCAC AAGGTATTAG AGACTGAGGG GCACCCCGGC ATGGTAAGCA GGAGCCATCG CGAAG
EOF
${GOALIGN} random -n 5 -l 75 --seed 10 | ${GOALIGN} reformat phylip --one-line > result
diff -q -b result expected
rm -f expected result mapfile
echo "->goalign reformat phylip --no-block --one-line"
cat > expected <<EOF
5 75
Seq0000 GATTAATTTGCCGTAGGCCAGAATCTGAAGATCGAACACTTTAAGTTTTCACTTCTAATGGAGAGGACTAGTTCA
Seq0001 TACTTTTTAAACACTTTTACATCGATGTCGGACCTAAGTATTGAGTACAACGGTGTATTCCAGCGGTGGAGAGGT
Seq0002 CTATTTTTCCGGTTGAAGGACTCTAGAGCTGTAAAGGGTATGGCCATGTGCTAAGCGCGGGCGGATTGCTGTTGG
Seq0003 AGCAAGGTTAAATACTCGGCAATGCCCCATGATCCCCCAAGGACAATAAGAGCGAAGTTAGAACAAATGAACCCC
Seq0004 GAGTGGAGGCTTTATGGCACAAGGTATTAGAGACTGAGGGGCACCCCGGCATGGTAAGCAGGAGCCATCGCGAAG
EOF
${GOALIGN} random -n 5 -l 75 --seed 10 | ${GOALIGN} reformat phylip --one-line --no-block > result
diff -q -b result expected
rm -f expected result mapfile
echo "->goalign reformat phylip --no-block"
cat > expected <<EOF
5 75
Seq0000 GATTAATTTGCCGTAGGCCAGAATCTGAAGATCGAACACTTTAAGTTTTCACTTCTAATG
Seq0001 TACTTTTTAAACACTTTTACATCGATGTCGGACCTAAGTATTGAGTACAACGGTGTATTC
Seq0002 CTATTTTTCCGGTTGAAGGACTCTAGAGCTGTAAAGGGTATGGCCATGTGCTAAGCGCGG
Seq0003 AGCAAGGTTAAATACTCGGCAATGCCCCATGATCCCCCAAGGACAATAAGAGCGAAGTTA
Seq0004 GAGTGGAGGCTTTATGGCACAAGGTATTAGAGACTGAGGGGCACCCCGGCATGGTAAGCA
GAGAGGACTAGTTCA
CAGCGGTGGAGAGGT
GCGGATTGCTGTTGG
GAACAAATGAACCCC
GGAGCCATCGCGAAG
EOF
${GOALIGN} random -n 5 -l 75 --seed 10 | ${GOALIGN} reformat phylip --no-block > result
diff -q -b result expected
rm -f expected result mapfile
echo "->goalign reformat phylip spaces tabs"
cat > expected <<EOF
5 5
S-e-q-0-0-00 GATTA
Se-q00-0-1 ATTTG
Se-q00-0-2 CCGTA
Seq00-03 GGCCA
Seq-0004 GAATC
EOF
cat > input.fa <<EOF
> S e q 0 0 00
GATTA
> Se q00 0 1
ATTTG
> Se q00 0 2
CCGTA
> Seq00 03
GGCCA
>Seq 0004
GAATC
EOF
${GOALIGN} reformat phylip -i input.fa --clean-names > result
diff -q -b result expected
rm -f expected result mapfile input.fa
echo "->goalign reformat fasta spaces tabs"
cat > expected <<EOF
>S-e-q-0-0-00-
GATTA
>Se-q00-0-2-
CCGTA
>Seq00-03-
GGCCA
>Se-q-0004
GAATC
EOF
cat > input.fa <<EOF
> S e q 0 0 00[]();.,
GATTA
> Se q00 0 2 []();.,
CCGTA
> Seq00 03[]();.,
GGCCA
>Se q[]();.,0004
GAATC
EOF
${GOALIGN} reformat fasta -i input.fa --clean-names > result
diff -q -b result expected
rm -f expected result mapfile input.fa
echo "->goalign reformat fasta spaces tabs"
cat > expected <<EOF
>seq1-A-simple-comment-to-be-removed?-
GATTA
>seq2-A-second-comment-Maybe-a-wrong-sequence-to-be-updated?-
CCGTA
EOF
cat > input.fa <<EOF
> seq1 [A simple comment; to be removed?]
GATTA
> seq2 [A second comment] (Maybe a wrong sequence: to be updated?)
CCGTA
EOF
${GOALIGN} reformat fasta -i input.fa --clean-names > result
diff -q -b result expected
rm -f expected result mapfile input.fa
echo "->goalign reformat phylip strict"
cat > expected <<EOF
5 5
Seq0000 GATTA
Seq0001 ATTTG
Seq0002 CCGTA
Seq0003 GGCCA
Seq0004 GAATC
EOF
${GOALIGN} random -n 5 -l 5 --seed 10 | ${GOALIGN} reformat phylip --output-strict > result
diff -q -b result expected
rm -f expected result mapfile
echo "->goalign reformat nexus"
cat > expected <<EOF
#NEXUS
begin data;
dimensions ntax=5 nchar=5;
format datatype=dna;
matrix
Seq0000 GATTA
Seq0001 ATTTG
Seq0002 CCGTA
Seq0003 GGCCA
Seq0004 GAATC
;
end;
EOF
${GOALIGN} random -n 5 -l 5 --seed 10 | ${GOALIGN} reformat nexus > result
diff -q -b result expected
rm -f expected result mapfile
echo "->goalign reformat fasta from clustal"
## Sample file from t-coffee:
## https://github.com/cbcrg/tcoffee/blob/master/examples/sample_aln2.aln
cat > input <<EOF
CLUSTAL W (1.83) multiple sequence alignment
1cms --GEVASVPLTNYLDSQYFGKIYLGTPPQEFTVLFDTGSSDFWVPSIYCK
4pep ----IGDEPLENYLDTEYFGTIGIGTPAQDFTVIFDTGSSNLWVPSVYCS
4ape -STGSATTTPIDSLDDAYITPVQIGTPAQTLNLDFDTGSSDLWVFSSETT
3app AASGVATNTPTAN-DEEYITPVTIG--GTTLNLNFDTGSADLWVFSTELP
2apr AGVGTVPMTDYGN-DIEYYGQVTIGTPGKKFNLDFDTGSSDLWIASTLCT
1cms_1 -----------------YTGSLHWVPVTVQQYWQFTVDS---VTISGVVV
* : * ..* *
1cms SNACKNHQRFDPR-KSSTFQNLGKPLSIHYGTG-SMQGILGYDTVTVSNI
4pep SLACSDHNQFNPD-DSSTFEATSQELSITYGTG-SMTGILGYDTVQVGGI
4ape ASEVDGQTIYTPSKSTTAKLLSGATWSISYGDGSSSSGDVYTDTVSVGGL
3app ASQQSGHSVYNP--SATGKELSGYTWSISYGDGSSASGNVFTDSVTVGGV
2apr NCG-SGQTKYDPN-QSSTYQADGRTWSISYGDGSSASGILAKDNVNLGGL
1cms_1 ACEGGCQAILDTG-TSKLVGPSSDILNIQQAIG-ATQNQYGEFDIDCDNL
: . :. . .* . * : . : ..:
1cms VDIQQTVGLSTQEPGDVFTYAEFDGILGMAYPSLASEY---SIPVFDNMM
4pep SDTNQIFGLSETEPGSFLYYAPFDGILGLAYPSISASG---ATPVFDNLW
4ape TVTGQAVESAKKVSSSFTEDSTIDGLLGLAFSTLNTVSPTQQKTFFDNAK
3app TAHGQAVQAAQQISAQFQQDTNNDGLLGLAFSSINTVQPQSQTTFFDTVK
2apr LIKGQTIELAKREAASFASGPN-DGLLGLGFDTITTVR--GVKTPMDNLI
1cms_1 SYMPTVVFEINGKMYPLTPSAYTSQDQGFCTSGFQSEN------HSQKWI
. . . . *: : : :.
1cms NRHLVAQDLFSVYMDRNGQE--SMLTLGAIDPSY
4pep DQGLVSQDLFSVYLSSNDDSG-SVVLLGGIDSSY
4ape AS--LDSPVFTADLGYHAPG---TYNFGFIDTTA
3app SS--LAQPLFAVALKHQQPG---VYDFGFIDSSK
2apr SQGLISRPIFGVYLGKAKNGGGGEYIFGGYDSTK
1cms_1 LGDVFIREYYSVFDRANNLVG----LAKAI----
. : .
EOF
cat > expected <<EOF
>1cms
--GEVASVPLTNYLDSQYFGKIYLGTPPQEFTVLFDTGSSDFWVPSIYCKSNACKNHQRFDPR-KSSTFQNLGKPLSIHY
GTG-SMQGILGYDTVTVSNIVDIQQTVGLSTQEPGDVFTYAEFDGILGMAYPSLASEY---SIPVFDNMMNRHLVAQDLF
SVYMDRNGQE--SMLTLGAIDPSY
>4pep
----IGDEPLENYLDTEYFGTIGIGTPAQDFTVIFDTGSSNLWVPSVYCSSLACSDHNQFNPD-DSSTFEATSQELSITY
GTG-SMTGILGYDTVQVGGISDTNQIFGLSETEPGSFLYYAPFDGILGLAYPSISASG---ATPVFDNLWDQGLVSQDLF
SVYLSSNDDSG-SVVLLGGIDSSY
>4ape
-STGSATTTPIDSLDDAYITPVQIGTPAQTLNLDFDTGSSDLWVFSSETTASEVDGQTIYTPSKSTTAKLLSGATWSISY
GDGSSSSGDVYTDTVSVGGLTVTGQAVESAKKVSSSFTEDSTIDGLLGLAFSTLNTVSPTQQKTFFDNAKAS--LDSPVF
TADLGYHAPG---TYNFGFIDTTA