diff --git a/x-pack/plugins/fleet/common/constants/routes.ts b/x-pack/plugins/fleet/common/constants/routes.ts
index 61d50d0f9e073..4b40b4fb51092 100644
--- a/x-pack/plugins/fleet/common/constants/routes.ts
+++ b/x-pack/plugins/fleet/common/constants/routes.ts
@@ -171,11 +171,6 @@ export const ENROLLMENT_API_KEY_ROUTES = {
   LIST_PATTERN: `${API_ROOT}/enrollment_api_keys`,
   INFO_PATTERN: `${API_ROOT}/enrollment_api_keys/{keyId}`,
   DELETE_PATTERN: `${API_ROOT}/enrollment_api_keys/{keyId}`,
-  // deprecated since 8.0
-  CREATE_PATTERN_DEPRECATED: `${API_ROOT}/enrollment-api-keys`,
-  LIST_PATTERN_DEPRECATED: `${API_ROOT}/enrollment-api-keys`,
-  INFO_PATTERN_DEPRECATED: `${API_ROOT}/enrollment-api-keys/{keyId}`,
-  DELETE_PATTERN_DEPRECATED: `${API_ROOT}/enrollment-api-keys/{keyId}`,
 };
 
 export const UNINSTALL_TOKEN_ROUTES = {
diff --git a/x-pack/plugins/fleet/server/routes/enrollment_api_key/index.ts b/x-pack/plugins/fleet/server/routes/enrollment_api_key/index.ts
index bcf4448420919..2d4c7c990c531 100644
--- a/x-pack/plugins/fleet/server/routes/enrollment_api_key/index.ts
+++ b/x-pack/plugins/fleet/server/routes/enrollment_api_key/index.ts
@@ -154,72 +154,4 @@ export const registerRoutes = (router: FleetAuthzRouter) => {
       },
       postEnrollmentApiKeyHandler
     );
-
-  router.versioned
-    .get({
-      path: ENROLLMENT_API_KEY_ROUTES.INFO_PATTERN_DEPRECATED,
-      fleetAuthz: {
-        fleet: { readEnrollmentTokens: true },
-      },
-      // @ts-expect-error TODO(https://github.com/elastic/kibana/issues/196095): Replace {RouteDeprecationInfo}
-      deprecated: true,
-    })
-    .addVersion(
-      {
-        version: API_VERSIONS.public.v1,
-        validate: { request: GetOneEnrollmentAPIKeyRequestSchema },
-      },
-      getOneEnrollmentApiKeyHandler
-    );
-
-  router.versioned
-    .delete({
-      path: ENROLLMENT_API_KEY_ROUTES.DELETE_PATTERN_DEPRECATED,
-      fleetAuthz: {
-        fleet: { allAgents: true },
-      },
-      // @ts-expect-error TODO(https://github.com/elastic/kibana/issues/196095): Replace {RouteDeprecationInfo}
-      deprecated: true,
-    })
-    .addVersion(
-      {
-        version: API_VERSIONS.public.v1,
-        validate: { request: DeleteEnrollmentAPIKeyRequestSchema },
-      },
-      deleteEnrollmentApiKeyHandler
-    );
-
-  router.versioned
-    .get({
-      path: ENROLLMENT_API_KEY_ROUTES.LIST_PATTERN_DEPRECATED,
-      fleetAuthz: {
-        fleet: { readEnrollmentTokens: true },
-      },
-      // @ts-expect-error TODO(https://github.com/elastic/kibana/issues/196095): Replace {RouteDeprecationInfo}
-      deprecated: true,
-    })
-    .addVersion(
-      {
-        version: API_VERSIONS.public.v1,
-        validate: { request: GetEnrollmentAPIKeysRequestSchema },
-      },
-      getEnrollmentApiKeysHandler
-    );
-
-  router.versioned
-    .post({
-      path: ENROLLMENT_API_KEY_ROUTES.CREATE_PATTERN_DEPRECATED,
-      fleetAuthz: {
-        fleet: { allAgents: true },
-      },
-      // @ts-expect-error TODO(https://github.com/elastic/kibana/issues/196095): Replace {RouteDeprecationInfo}
-      deprecated: true,
-    })
-    .addVersion(
-      {
-        version: API_VERSIONS.public.v1,
-        validate: { request: PostEnrollmentAPIKeyRequestSchema },
-      },
-      postEnrollmentApiKeyHandler
-    );
 };
diff --git a/x-pack/test/fleet_api_integration/apis/enrollment_api_keys/crud.ts b/x-pack/test/fleet_api_integration/apis/enrollment_api_keys/crud.ts
index 9cb11df2b34de..24d1520d8d6f8 100644
--- a/x-pack/test/fleet_api_integration/apis/enrollment_api_keys/crud.ts
+++ b/x-pack/test/fleet_api_integration/apis/enrollment_api_keys/crud.ts
@@ -285,32 +285,5 @@ export default function (providerContext: FtrProviderContext) {
         });
       });
     });
-
-    describe('deprecated API', () => {
-      let keyId: string;
-      before(async () => {
-        const { body: apiResponse } = await supertest
-          .post(`/api/fleet/enrollment-api-keys`)
-          .set('kbn-xsrf', 'xxx')
-          .send({
-            policy_id: 'policy1',
-          })
-          .expect(200);
-        keyId = apiResponse.item.id;
-      });
-
-      it('should get and delete with deprecated API', async () => {
-        await supertest.get(`/api/fleet/enrollment-api-keys`).set('kbn-xsrf', 'xxx').expect(200);
-        await supertest
-          .get(`/api/fleet/enrollment-api-keys/${ENROLLMENT_KEY_ID}`)
-          .set('kbn-xsrf', 'xxx')
-          .expect(200);
-
-        await supertest
-          .delete(`/api/fleet/enrollment-api-keys/${keyId}`)
-          .set('kbn-xsrf', 'xxx')
-          .expect(200);
-      });
-    });
   });
 }