find the leaf area #1006
Replies: 3 comments 4 replies
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Hi @SadafSafder23, for the grass plant, following the general steps of this tutorial would get you the measurements you are aiming to do: https://plantcv.readthedocs.io/en/stable/tutorials/sorghum_morphology_tutorial/ The other plant is more complicated for a few reasons. The tools used in the tutorial above work well on thin grass leaves, but skeletonization (as we have it implemented) will not work as intended on the oval-shaped leaves in the first image because you will get a branch segment corresponding to the long axis and another mostly perpendicular corresponding to the short axis. The other issue is that the leaves overlap, which will further break the assumptions of the Because the leaves in the first image are nicely spread out to either side of the stem, you may have good luck with watershed segmentation: https://plantcv.readthedocs.io/en/stable/tutorials/watershed_segmentation_tutorial/ |
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Thank you @nfahlgren for your response, I'm sorry, I'm away that's why could not respond. |
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To elaborate on the leaf area algorithm a bit more, the function For the stem length, I recommend using the To convert from pixels to centimeter you must have a size marker in your images that have a real, known area/length. Then you can scale the units with simple division once you have your data table. |
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Hi,
I am working on phenotyping and calculate the leaf area, leaf angle and stem length now I have need to calculate the leaf area using Plantcv, can anyone here to help me what function is used here to calculate each leaf area in a plant. here's the some images of the plant.
Kind Regards,
Sadaf
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