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Error for most trinotateR functions: Error in eval(bysub, x, parent.frame()) : object 'go' not found #2

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browngcm opened this issue Aug 31, 2017 · 1 comment

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@browngcm
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browngcm commented Aug 31, 2017

Hello,
I just generated a Trinotate annotation report, and I'm trying to summarize the findings of the report with trinotateR. I can run all of the functions outlined in README.md, but when I try to summarize other aspects of the report using summary_GO, summary blast, summary_pfam, etc, I encounter this error:

summary_GO(x)
Error in eval(bysub, x, parent.frame()) : object 'go' not found

For each of the summary functions, do I need to go through the same steps as outlined in the README.md file for the pfam summary table?

@cstubben
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Yes, you need to split the annotations into separate rows and columns first and then summarize. This should work for GO columns and the default is gene_ontology_blast.

go <- split_go(x)
summary_GO(go)

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