From 1f4c0ab2356da7e172b19ec1b79a87e06cc8c1eb Mon Sep 17 00:00:00 2001 From: Connor French Date: Wed, 26 Jun 2024 15:35:35 -0400 Subject: [PATCH] add quickstart --- docs/index.md | 341 +++++++++++++++++- docs/index.qmd | 224 ++++++++++-- .../draw-mutations-output-1.svg | 1 + .../figure-commonmark/draw-tree-output-1.svg | 1 + .../figure-commonmark/localities-output-1.png | Bin 0 -> 26357 bytes .../plot-landscape-output-1.png | Bin 0 -> 92971 bytes .../timeslices-1-output-1.png | Bin 0 -> 46504 bytes .../timeslices1-output-1.png | Bin 0 -> 46504 bytes 8 files changed, 542 insertions(+), 25 deletions(-) create mode 100644 docs/index_files/figure-commonmark/draw-mutations-output-1.svg create mode 100644 docs/index_files/figure-commonmark/draw-tree-output-1.svg create mode 100644 docs/index_files/figure-commonmark/localities-output-1.png create mode 100644 docs/index_files/figure-commonmark/plot-landscape-output-1.png create mode 100644 docs/index_files/figure-commonmark/timeslices-1-output-1.png create mode 100644 docs/index_files/figure-commonmark/timeslices1-output-1.png diff --git a/docs/index.md b/docs/index.md index 03a9131..9952d6e 100644 --- a/docs/index.md +++ b/docs/index.md @@ -126,18 +126,353 @@ package to reduce the number of dependencies: from spaceprime import analysis ``` -> \[!IMPORTANT\] -> Make sure to install the relevant analysis dependencies with -> `pip install spaceprime[analysis]` or +> \[!IMPORTANT\] Make sure to install the relevant analysis dependencies +> with `pip install spaceprime[analysis]` or > `conda install -c conda-forge spaceprime[analysis]`. ## Quickstart +> \[!NOTE\] This quickstart guide assumes you have a basic understanding +> of Python. If you are an R user, please refer to the [spaceprime for R +> Users vignette](https://connor-french.github.io/spaceprime) for an +> overview of *spaceprime* with the necessary Python concepts explained. + +### 1. Download data + +The data we’re using in this example are a +[GeoTiff](https://en.wikipedia.org/wiki/GeoTIFF) raster file of habitat +suitability values and a [GeoJSON](https://geojson.org/) file containing +geographic localities and metadata for this cute frog, *Phyllomedusa +distincta*: + + + +Follow the link to download the [projections.tif +file](https://github.com/connor-french/spaceprime/raw/main/spaceprime/data/projections.tif). +You do not need to download the +[localities.geojson](https://raw.githubusercontent.com/connor-french/spaceprime/main/spaceprime/data/localities.geojson) +file, as it is read in from the web in the code below. + +The raster file contains 23 layers, where each layer is a projection of +the [habitat suitability model (aka species distribution model or +ecological niche +model)](https://en.wikipedia.org/wiki/Species_distribution_modelling) to +a time slice in the past, ranging from the present day to 22,000 years +ago in 1,000 year intervals. The habitat suitability values range from +zero to one, where zero represents no suitability for the species and +one represents perfect suitability. In the following plots, **yellow +represents higher suitability and purple represents lower suitability**. +Here are a few time slices of the model: + + + +The GeoJSON file contains geographic localities of *P. distincta* in the +Brazilian Atlantic Forest, along with metadata about each locality. Here +are the localities plotted on top of the present-day habitat suitability +model: + + + +### 2. Read in packages and data + +Now that we have our data, let’s read in the packages and data we’ll be +using. Make sure to replace the `projections.tif` file path with the +path to the file on your system: + ``` python import spaceprime as sp import geopandas as gpd +import rasterio + + +r = rasterio.open("projections.tif") +locs = gpd.read_file("https://raw.githubusercontent.com/connor-french/spaceprime/main/spaceprime/data/localities.geojson") +``` + +### 3. Set up the demographic model + +Next, we’ll convert the habitat suitability values into deme sizes, so +each cell in the raster will represent a deme in our model. We’ll use a +linear transformation to convert the suitability values to deme sizes, +where the suitability value is multiplied by a constant to get the deme +size. The constant is the maximum local deme size, which we set to 1000. +For more on transformations, see the [suitability to deme size +transformation functions vignette](trans-fns.md). + +``` python +d = sp.raster_to_demes(r, transformation="linear", max_local_size=1000) +``` + +Now that we have our deme sizes, we can set up the demographic model. +The model that *spaceprime* uses is a two-dimensional stepping-stone +model with a global migration rate of 0.001 between neighboring demes. +The global rate by default is scaled, where demes exchange the same +number of migrants with their neighbors, regardless of deme size. To +change this behavior, set `scale=false`. Using a single value for the +`timesteps` argument tells spaceprime that 1000 generations passes in +between each raster time step in the model, which we are assuming here. +This step may take a few seconds (10-15 seconds on my machine) to run. + +``` python +# initialize the model +demo = sp.spDemography() + +# populate the spDemography object with the deme sizes and migration rates +demo.stepping_stone_2d(d, rate=0.001, timesteps=1000) +``` + +After initializing the spatial component of the simulation, it’s +desirable to add one or more ancestral populations to the model. This is +done by providing a list of ancestral population sizes and the time (in +generations) at which the spatially distributed demes migrate into the +ancestral population(s). The following code adds a single ancestral +population of 100,000 individuals that demes merge into 23,000 +generations in the past: + +``` python +# add ancestral population +demo.add_ancestral_populations([100000], 23000) +``` + +### 4. Inspect your model + +Now that we have our demographic model set up, we can inspect it to make +sure it looks as expected. *spaceprime* has a series of `plot_()` +functions that make this easier. + +#### `plot_landscape()` + +`plot_landscape()` plots the deme sizes in space, which allows you to +quickly inspect whether the transformation you applied to your habitat +suitability map make sense. Here, we provide the demographic model +object, the raster object, the index of the time slice to plot (0 for +the present day in this case), and `basemap=True` to add an +[OpenStreetMap](https://www.openstreetmap.org/) basemap, providing +geographic context to the plot. If you don’t have an internet +connection, set `basemap=False` (the default) to plot without the +basemap. + +``` python +sp.plot_landscape(demo, r, 0, basemap=True) +``` + + + +#### `plot_model()` + +`plot_model()` plots the deme sizes on a +[folium](https://python-visualization.github.io/folium/latest/index.html) +interactive map, with precise deme sizes and outgoing migration rates +for each deme present in a popup. *Note*- the below is rendered as an +image, but the actual plot is interactive. + +``` python +sp.plot_model(demo, r, 0) +``` + +### 4. Simulate genetic data + +Before simulating this demography, we need to create a sample dictionary +that translates the empirical sampling localities to the model’s deme +indices and maps those to the number of samples to take from each deme. +By default, this function sets the number of individuals to sample from +each deme to the number of empirical localities in that deme. The +`coords_to_sample_dict()` function also returns two other dictionaries +that are not used in this example, so we’ll ignore them. + +``` python +sample_dict, _, _ = sp.coords_to_sample_dict(r, locs) +``` + +Now we get to simulate! The first task is to simulate the ancestry of +the samples using the coalescent. All of the hard work is done through +`msprime`’s `sim_ancestry()` function, for which `spaceprime` provides a +convenience wrapper. This function returns a \[tskit +TreeSequence(https://tskit.dev/tskit/docs/stable/python-api.html#trees-and-tree-sequences), +which “represents a sequence of correlated evolutionary trees along a +genome” and is and incredibly powerful and compact data representation +for population genomic analyses. The minimum number of arguments +required for this function are the sample dictionary and the demographic +model. The sequence length is also necessary for overlaying mutations in +the next step. We’ll set `record_provenance` to False to decrease the +memory overhead of storing a bunch of metadata about the simulation. + +This step may take a minute or so to run. + +``` python +sim = sp.sim_ancestry(samples=sample_dict, demography=demo, sequence_length=1e5, record_provenance=False, random_seed=42) + +sim ``` +
+ +
+
+ +| Tree Sequence | | +|--------------------------------------------------------------------------------------|-------------| +| Trees | 1 | +| Sequence Length | 100000.0 | +| Time Units | generations | +| Sample Nodes | 344 | +| Total Size | 205.8 KiB | +| Metadata | No Metadata | + +
+ +
+ +| Table | Rows | Size | Has Metadata | +|-------------|------|-----------|:------------:| +| Edges | 686 | 21.4 KiB | | +| Individuals | 172 | 4.7 KiB | | +| Migrations | 0 | 8 Bytes | | +| Mutations | 0 | 16 Bytes | | +| Nodes | 687 | 18.8 KiB | | +| Populations | 3481 | 155.4 KiB | ✅ | +| Provenances | 0 | 16 Bytes | | +| Sites | 0 | 16 Bytes | | + +
+ +
+
+ + +We’ll take a peak at a single tree from the TreeSequence object to see +what it looks like. The `draw_tree()` function is a convenience function +that uses `msprime`’s `draw_text()` function to plot a single tree from +the TreeSequence object. Here, I removed the node labels because there +are tons of nodes that crowd the plot and we’re only interested in the +tree structure. + +``` python +first_tree = sim.first() +node_labels = {node.id: "" for node in sim.nodes()} +first_tree.draw_svg(y_axis=True, size=(600, 400), node_labels=node_labels) +``` + + + +Overlaying mutations after simulating ancestry isn’t necessary for +calculating genetic summary statistics on a TreeSequence, but it is +necessary if you would like to compare your simulations with empirical +data that are represented as a table of genotypes rather than a +TreeSequence. The `sim_mutations()` function overlays mutations on the +TreeSequence object returned by `sim_ancestry()` and requires the +mutation rate. The mutation rate is the number of mutations per base +pair per generation. For this example, we’ll use a mutation rate of +1e-9, which is a common rate for vertebrates. You can see from the table +that the tree sequence has some mutations! + +``` python +sim = sp.sim_mutations(sim, rate=1e-10, random_seed=490) + +sim +``` + +
+ +
+
+ +| Tree Sequence | | +|--------------------------------------------------------------------------------------|-------------| +| Trees | 1 | +| Sequence Length | 100000.0 | +| Time Units | generations | +| Sample Nodes | 344 | +| Total Size | 209.4 KiB | +| Metadata | No Metadata | + +
+ +
+ +| Table | Rows | Size | Has Metadata | +|-------------|------|-----------|:------------:| +| Edges | 686 | 21.4 KiB | | +| Individuals | 172 | 4.7 KiB | | +| Migrations | 0 | 8 Bytes | | +| Mutations | 48 | 1.8 KiB | | +| Nodes | 687 | 18.8 KiB | | +| Populations | 3481 | 155.4 KiB | ✅ | +| Provenances | 1 | 763 Bytes | | +| Sites | 48 | 1.2 KiB | | + +
+ +
+
+ + +The mutations will be spread across the TreeSequence, but there are a +few on the first tree. + +``` python +first_tree_mut = sim.first() + +node_labels = {node.id: "" for node in sim.nodes()} +first_tree_mut.draw_svg(y_axis=True, size=(600, 400), node_labels=node_labels) +``` + + + +From here, you have a few options. You can: + +- Use the `analysis` module to calculate genetic summary statistics on + the TreeSequence object. For more information on how to use the + `analysis` module, see the [analysis module documentation](). +- Save the TreeSequence to use later or analyze on a platform like + [tskit](https://tskit.dev/tskit/docs/stable/introduction.html) with + `sim.dump(file_path_to_write_to)`. +- Convert the TreeSequence with mutations to a genotype matrix for use + in a program like + [scikit-allel](https://scikit-allel.readthedocs.io/en/stable/) with + `sim.genotype_matrix()`. For more information on how to use this + function, see the [tskit + documentation](https://tskit.dev/tskit/docs/stable/python-api.html#tskit.TreeSequence.genotype_matrix). +- Export the TreeSequence with mutations to a VCF file using + `sim.write_vcf`. For more information on how to use this function, see + the [tskit + documentation](https://tskit.dev/tskit/docs/stable/python-api.html#tskit.TreeSequence.write_vcf). + +> \[!TODO\] add a link to the analysis module documentation when it’s +> ready. + ## Report Issues diff --git a/docs/index.qmd b/docs/index.qmd index 486f0ed..2c81d5b 100644 --- a/docs/index.qmd +++ b/docs/index.qmd @@ -4,33 +4,27 @@ format: gfm: eval: false echo: true - --- - [![image](https://img.shields.io/pypi/v/spaceprime.svg)](https://pypi.python.org/pypi/spaceprime) - **A python package to facilitate spatially explicit coalescent modeling in *msprime*** - - Free software: MIT License - Documentation: ## *Overview* -*spaceprime* is a Python package that facilitates the use of **spatially explicit coalescent modeling** in the *msprime* library. The package is designed to make it easier for practitioners to **convert spatial maps of habitat suitability into extensible two-dimensional stepping-stone models of gene flow**, where each pixel of the map represents a deme and demes are able to migrate with their neighbors. Demes and migration rates are able to change over time according to habitat suitability model projections to different time periods. These demographic parameters are then used to simulate genetic data under a coalescent model with *msprime* as the simulator, which can be used to infer the demographic history of the population. The package is designed to be **user-friendly and intuitive**, allowing users to easily simulate and analyze spatially explicit genetic data. -This page provides an overview of the package and its main features. For more detailed information, please refer to the [documentation](https://connor-french.github.io/spaceprime). +*spaceprime* is a Python package that facilitates the use of **spatially explicit coalescent modeling** in the *msprime* library. The package is designed to make it easier for practitioners to **convert spatial maps of habitat suitability into extensible two-dimensional stepping-stone models of gene flow**, where each pixel of the map represents a deme and demes are able to migrate with their neighbors. Demes and migration rates are able to change over time according to habitat suitability model projections to different time periods. These demographic parameters are then used to simulate genetic data under a coalescent model with *msprime* as the simulator, which can be used to infer the demographic history of the population. The package is designed to be **user-friendly and intuitive**, allowing users to easily simulate and analyze spatially explicit genetic data. + +This page provides an overview of the package and its main features. For more detailed information, please refer to the [documentation](https://connor-french.github.io/spaceprime). **Note for R users**: *spaceprime* is coded in Python, yet many interested users may come from an R background. I have a [*spaceprime* for R users]() vignette (COMING SOON) that provides a brief introduction to the Python concepts necessary to use *spaceprime* in a practical walk-through of an example analysis. Additionally, it is possible to use Python code in R using the *reticulate* package. For more information on how to use Python code in R, see the [reticulate documentation](https://rstudio.github.io/reticulate/). ## Main features -*spaceprime* includes a number of features: -- **Convert habitat suitability values into demographic parameters**, including deme sizes, migration rates, and their change through time using **very little code**. Code complexity does not increase with model complexity, allowing users to focus on the biological questions they are interested in. -- Simulate spatially explicit genetic data under a coalescent model with *msprime*. The modeling approach is **fully coalescent** with no forward-time component, allowing for **computationally efficient simulations** of large spatially explicit models. -- Visualize demographic models to facilitate model interpretation and model checking. -- Compute genetic summary statistics for simulated and empirical data to facilitate comparison with empirical data. -- Extensibility: *spaceprime* is designed to be interoperable with *msprime*, where users can setup a model with *spaceprime*, then customize it using the full range of *msprime* functionality. + +*spaceprime* includes a number of features: - **Convert habitat suitability values into demographic parameters**, including deme sizes, migration rates, and their change through time using **very little code**. Code complexity does not increase with model complexity, allowing users to focus on the biological questions they are interested in. - Simulate spatially explicit genetic data under a coalescent model with *msprime*. The modeling approach is **fully coalescent** with no forward-time component, allowing for **computationally efficient simulations** of large spatially explicit models. - Visualize demographic models to facilitate model interpretation and model checking. - Compute genetic summary statistics for simulated and empirical data to facilitate comparison with empirical data.\ +- Extensibility: *spaceprime* is designed to be interoperable with *msprime*, where users can setup a model with *spaceprime*, then customize it using the full range of *msprime* functionality. ## Installation @@ -72,7 +66,7 @@ To install the `analysis` module, which is imported separately from the main pac pip install spaceprime[analysis] ``` -or +or ```{bash} conda install -c conda-forge spaceprime[analysis] @@ -92,34 +86,220 @@ To use the `analysis` module, which is imported separately from the main package from spaceprime import analysis ``` -> [!IMPORTANT] +> [!IMPORTANT] > Make sure to install the relevant analysis dependencies with `pip install spaceprime[analysis]` or `conda install -c conda-forge spaceprime[analysis]`. ## Quickstart -### 1. Read in packages and data +> [!NOTE] +> This quickstart guide assumes you have a basic understanding of Python. If you are an R user, please refer to the [spaceprime for R Users vignette](https://connor-french.github.io/spaceprime) for an overview of *spaceprime* with the necessary Python concepts explained. + +### 1. Download data + +The data we're using in this example are a [GeoTiff](https://en.wikipedia.org/wiki/GeoTIFF) raster file of habitat suitability values and a [GeoJSON](https://geojson.org/) file containing geographic localities and metadata for this cute frog, *Phyllomedusa distincta*: + +![](images/pdistincta.jpg){width=50% fig-align="center"} + +Follow the link to download the [projections.tif file](https://github.com/connor-french/spaceprime/raw/main/spaceprime/data/projections.tif). You do not need to download the [localities.geojson](https://raw.githubusercontent.com/connor-french/spaceprime/main/spaceprime/data/localities.geojson) file, as it is read in from the web in the code below. + + +The raster file contains 23 layers, where each layer is a projection of the [habitat suitability model (aka species distribution model or ecological niche model)](https://en.wikipedia.org/wiki/Species_distribution_modelling) to a time slice in the past, ranging from the present day to 22,000 years ago in 1,000 year intervals. The habitat suitability values range from zero to one, where zero represents no suitability for the species and one represents perfect suitability. In the following plots, **yellow represents higher suitability and purple represents lower suitability**. Here are a few time slices of the model: + +```{python} +#| label: timeslices1 +#| eval: true +#| echo: false + +import spaceprime as sp #putting this here for use later +import rasterio +import matplotlib.pyplot as plt -[Packages we're loading and why] +r = rasterio.open("../spaceprime/data/projections.tif") -The data we're using in this example is a GeoTiff raster file of habitat suitability values for this cute frog, *Phyllomedusa distincta*: +fig, axes = plt.subplots(2, 2, figsize=(10, 8)) -![](images/pdistincta.jpg) +for i, ax in enumerate(axes.flatten()): + ax.imshow(r.read([1, 7, 14, 21][i]), cmap="viridis") + ax.set_title(f"{[0, 6, 13, 20][i]} kya") -You can download the data [here](). +plt.tight_layout() +plt.show() +``` -[Explanation of sample data] +The GeoJSON file contains geographic localities of *P. distincta* in the Brazilian Atlantic Forest, along with metadata about each locality. Here are the localities plotted on top of the present-day habitat suitability model: ```{python} -#| label: qs-import +#| label: localities +#| echo: false #| eval: true +import geopandas as gpd +import matplotlib.pyplot as plt +from rasterio.plot import show + + +locs = gpd.read_file("https://raw.githubusercontent.com/connor-french/spaceprime/main/spaceprime/data/localities.geojson") + +fig, ax = plt.subplots(figsize=(6, 6)) +r = rasterio.open("../spaceprime/data/projections.tif") +show(r.read(1), ax=ax, cmap="viridis", transform=r.transform) +locs.plot(ax=ax, color="red", markersize=50) +plt.show() + +``` + +### 2. Read in packages and data + +Now that we have our data, let's read in the packages and data we'll be using. Make sure to replace the `projections.tif` file path with the path to the file on your system: + +```{python} +#| label: import-data + import spaceprime as sp import geopandas as gpd +import rasterio +r = rasterio.open("projections.tif") +locs = gpd.read_file("https://raw.githubusercontent.com/connor-french/spaceprime/main/spaceprime/data/localities.geojson") ``` +### 3. Set up the demographic model + +Next, we'll convert the habitat suitability values into deme sizes, so each cell in the raster will represent a deme in our model. We'll use a linear transformation to convert the suitability values to deme sizes, where the suitability value is multiplied by a constant to get the deme size. The constant is the maximum local deme size, which we set to 1000. For more on transformations, see the [suitability to deme size transformation functions vignette](trans-fns.md). + +```{python} +#| label: raster-to-demes +#| eval: true + +d = sp.raster_to_demes(r, transformation="linear", max_local_size=1000) +``` + +Now that we have our deme sizes, we can set up the demographic model. The model that *spaceprime* uses is a two-dimensional stepping-stone model with a global migration rate of 0.001 between neighboring demes. The global rate by default is scaled, where demes exchange the same number of migrants with their neighbors, regardless of deme size. To change this behavior, set `scale=false`. Using a single value for the `timesteps` argument tells spaceprime that 1000 generations passes in between each raster time step in the model, which we are assuming here. This step may take a few seconds (10-15 seconds on my machine) to run. + +```{python} +#| label: demography +#| eval: true + +# initialize the model +demo = sp.spDemography() + +# populate the spDemography object with the deme sizes and migration rates +demo.stepping_stone_2d(d, rate=0.001, timesteps=1000) +``` + + +After initializing the spatial component of the simulation, it's desirable to add one or more ancestral populations to the model. This is done by providing a list of ancestral population sizes and the time (in generations) at which the spatially distributed demes migrate into the ancestral population(s). The following code adds a single ancestral population of 100,000 individuals that demes merge into 23,000 generations in the past: + +```{python} +#| label: anc-pops +#| eval: true + +# add ancestral population +demo.add_ancestral_populations([100000], 23000) +``` + +### 4. Inspect your model + +Now that we have our demographic model set up, we can inspect it to make sure it looks as expected. *spaceprime* has a series of `plot_()` functions that make this easier. + +#### `plot_landscape()` +`plot_landscape()` plots the deme sizes in space, which allows you to quickly inspect whether the transformation you applied to your habitat suitability map make sense. Here, we provide the demographic model object, the raster object, the index of the time slice to plot (0 for the present day in this case), and `basemap=True` to add an [OpenStreetMap](https://www.openstreetmap.org/) basemap, providing geographic context to the plot. If you don't have an internet connection, set `basemap=False` (the default) to plot without the basemap. + +```{python} +#| label: plot-landscape +#| eval: true + +sp.plot_landscape(demo, r, 0, basemap=True) +``` + +#### `plot_model()` +`plot_model()` plots the deme sizes on a [folium](https://python-visualization.github.io/folium/latest/index.html) interactive map, with precise deme sizes and outgoing migration rates for each deme present in a popup. *Note*- the below is rendered as an image, but the actual plot is interactive. + +```{python} +#| label: plot-model + +sp.plot_model(demo, r, 0) +``` + + +### 4. Simulate genetic data + +Before simulating this demography, we need to create a sample dictionary that translates the empirical sampling localities to the model's deme indices and maps those to the number of samples to take from each deme. By default, this function sets the number of individuals to sample from each deme to the number of empirical localities in that deme. The `coords_to_sample_dict()` function also returns two other dictionaries that are not used in this example, so we'll ignore them. + +```{python} +#| label: sample-dict +#| eval: true + +sample_dict, _, _ = sp.coords_to_sample_dict(r, locs) +``` + +Now we get to simulate! The first task is to simulate the ancestry of the samples using the coalescent. All of the hard work is done through `msprime`'s `sim_ancestry()` function, for which `spaceprime` provides a convenience wrapper. This function returns a [tskit TreeSequence(https://tskit.dev/tskit/docs/stable/python-api.html#trees-and-tree-sequences), which "represents a sequence of correlated evolutionary trees along a genome" and is and incredibly powerful and compact data representation for population genomic analyses. The minimum number of arguments required for this function are the sample dictionary and the demographic model. The sequence length is also necessary for overlaying mutations in the next step. We'll set `record_provenance` to False to decrease the memory overhead of storing a bunch of metadata about the simulation. + +This step may take a minute or so to run. + +```{python} +#| label: simulate +#| eval: true + +sim = sp.sim_ancestry(samples=sample_dict, demography=demo, sequence_length=1e5, record_provenance=False, random_seed=42) + +sim +``` + + +We'll take a peak at a single tree from the TreeSequence object to see what it looks like. The `draw_tree()` function is a convenience function that uses `msprime`'s `draw_text()` function to plot a single tree from the TreeSequence object. Here, I removed the node labels because there are tons of nodes that crowd the plot and we're only interested in the tree structure. + +```{python} +#| label: draw-tree +#| eval: true + +first_tree = sim.first() +node_labels = {node.id: "" for node in sim.nodes()} +first_tree.draw_svg(y_axis=True, size=(600, 400), node_labels=node_labels) +``` + + +Overlaying mutations after simulating ancestry isn't necessary for calculating genetic summary statistics on a TreeSequence, but it is necessary if you would like to compare your simulations with empirical data that are represented as a table of genotypes rather than a TreeSequence. The `sim_mutations()` function overlays mutations on the TreeSequence object returned by `sim_ancestry()` and requires the mutation rate. The mutation rate is the number of mutations per base pair per generation. For this example, we'll use a mutation rate of 1e-9, which is a common rate for vertebrates. You can see from the table that the tree sequence has some mutations! + +```{python} +#| label: mutations +#| eval: true + + +sim = sp.sim_mutations(sim, rate=1e-10, random_seed=490) + +sim +``` + +The mutations will be spread across the TreeSequence, but there are a few on the first tree. + +```{python} +#| label: draw-mutations +#| eval: true + + +first_tree_mut = sim.first() + +node_labels = {node.id: "" for node in sim.nodes()} +first_tree_mut.draw_svg(y_axis=True, size=(600, 400), node_labels=node_labels) + +``` + +From here, you have a few options. You can: + +- Use the `analysis` module to calculate genetic summary statistics on the TreeSequence object. For more information on how to use the `analysis` module, see the [analysis module documentation](). +- Save the TreeSequence to use later or analyze on a platform like [tskit](https://tskit.dev/tskit/docs/stable/introduction.html) with `sim.dump(file_path_to_write_to)`. +- Convert the TreeSequence with mutations to a genotype matrix for use in a program like [scikit-allel](https://scikit-allel.readthedocs.io/en/stable/) with `sim.genotype_matrix()`. For more information on how to use this function, see the [tskit documentation](https://tskit.dev/tskit/docs/stable/python-api.html#tskit.TreeSequence.genotype_matrix). +- Export the TreeSequence with mutations to a VCF file using `sim.write_vcf`. For more information on how to use this function, see the [tskit documentation](https://tskit.dev/tskit/docs/stable/python-api.html#tskit.TreeSequence.write_vcf). + + +> [!TODO] +> add a link to the analysis module documentation when it's ready. + + ## Report Issues -[https://github.com/connor-french/spaceprime/issues](https://github.com/connor-french/spaceprime/issues) + + \ No newline at end of file diff --git a/docs/index_files/figure-commonmark/draw-mutations-output-1.svg b/docs/index_files/figure-commonmark/draw-mutations-output-1.svg new file mode 100644 index 0000000..3d38d7a --- /dev/null +++ b/docs/index_files/figure-commonmark/draw-mutations-output-1.svg @@ -0,0 +1 @@ +Time 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