kmer.ipynb

{
 "cells": [
  {
   "cell_type": "code",
   "execution_count": 37,
   "id": "894cb716-2450-41f9-91f3-087fde111c61",
   "metadata": {},
   "outputs": [],
   "source": [
    "from collections import Counter\n",
    "from Bio import SeqIO\n",
    "from Bio.SeqUtils import lcc"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 29,
   "id": "5060888b-ff6e-40c9-b482-d1f612c3430a",
   "metadata": {},
   "outputs": [],
   "source": [
    "records = SeqIO.parse('/media/GenomicResearch/Issue/20211026_沙鹿童綜合Sal/Complete_genome/5A-sal-05_1.fa', 'fasta')\n",
    "record = next(records)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 30,
   "id": "ea81692e-621d-446a-89b8-a61ace34995b",
   "metadata": {},
   "outputs": [],
   "source": [
    "seq = record.seq"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 31,
   "id": "73e7586e-200d-4044-a5c4-88d3479b1930",
   "metadata": {},
   "outputs": [],
   "source": [
    "k = 31"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 32,
   "id": "7d992508-7df9-4c17-bcf7-605ef28a0e7c",
   "metadata": {},
   "outputs": [],
   "source": [
    "stop = len(seq) - k + 1"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 33,
   "id": "55f5e490-5bf8-4af9-a898-5b8251a6e347",
   "metadata": {},
   "outputs": [],
   "source": [
    "c = Counter(seq[i:i+k] for i in range(stop))"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 34,
   "id": "a8806d85-ee79-49b9-ba33-26d21aacc808",
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "[(Seq('ATGGTCATGGTCATGGTCATGGTCATGGTCA'), 10)]"
      ]
     },
     "execution_count": 34,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "c.most_common(1)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 42,
   "id": "5b0febe3-9f5f-43ad-bdcc-c0e23df88a27",
   "metadata": {},
   "outputs": [],
   "source": [
    "kmer_complexity = [(kmer, lcc.lcc_simp(kmer)) for kmer in c.keys()]"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 45,
   "id": "b4ec49b4-c5bf-40b8-965b-f1c4c166c490",
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "[(Seq('GCCACAGAATTCAGTATGTGGATACGCCCGT'), 1.997696811857739),\n",
       " (Seq('CCACAGAATTCAGTATGTGGATACGCCCGTT'), 1.997696811857739),\n",
       " (Seq('CACAGAATTCAGTATGTGGATACGCCCGTTG'), 1.997696811857739),\n",
       " (Seq('ACAGAATTCAGTATGTGGATACGCCCGTTGC'), 1.997696811857739),\n",
       " (Seq('CAGAATTCAGTATGTGGATACGCCCGTTGCA'), 1.997696811857739),\n",
       " (Seq('AACTGAGCGATAACACGCTGGCTTTGTATGC'), 1.997696811857739),\n",
       " (Seq('AGCGATAACACGCTGGCTTTGTATGCGCCAA'), 1.997696811857739),\n",
       " (Seq('GCGATAACACGCTGGCTTTGTATGCGCCAAA'), 1.997696811857739),\n",
       " (Seq('ATCAATGGATTACTCAACGCATTCTGCGGCG'), 1.997696811857739),\n",
       " (Seq('ATGCCCCACAACTGCGTTTTGAAGTGGGAAC'), 1.997696811857739),\n",
       " (Seq('TGCCCCACAACTGCGTTTTGAAGTGGGAACA'), 1.997696811857739),\n",
       " (Seq('GCTAAAAACGCCTGTGCATAACGTTGTCGCG'), 1.997696811857739),\n",
       " (Seq('CGAAAGTCGTGTATATGCACTCCGAGCGCTT'), 1.997696811857739),\n",
       " (Seq('TATATGCACTCCGAGCGCTTTGTGCAGGACA'), 1.997696811857739),\n",
       " (Seq('ATATGCACTCCGAGCGCTTTGTGCAGGACAT'), 1.997696811857739),\n",
       " (Seq('GAAGAGTTTAAACGCTACTACCGCTCCGTTG'), 1.997696811857739),\n",
       " (Seq('TACCTTTAACGCTCTGCTGGAAGGCAATCAG'), 1.997696811857739),\n",
       " (Seq('CTTTAACGCTCTGCTGGAAGGCAATCAGCAG'), 1.997696811857739),\n",
       " (Seq('CGCTCTGCTGGAAGGCAATCAGCAGATCATT'), 1.997696811857739),\n",
       " (Seq('GCTCTGCTGGAAGGCAATCAGCAGATCATTT'), 1.997696811857739),\n",
       " (Seq('GGCAATCAGCAGATCATTTTGACGTCGGATC'), 1.997696811857739),\n",
       " (Seq('GCAATCAGCAGATCATTTTGACGTCGGATCG'), 1.997696811857739),\n",
       " (Seq('CAATCAGCAGATCATTTTGACGTCGGATCGC'), 1.997696811857739),\n",
       " (Seq('CATTTTGACGTCGGATCGCTATCCGAAAGAG'), 1.997696811857739),\n",
       " (Seq('TTTGACGTCGGATCGCTATCCGAAAGAGATC'), 1.997696811857739),\n",
       " (Seq('AAGCGTCTACGCTCTAACGTACGTGAACTGG'), 1.997696811857739),\n",
       " (Seq('AGCGTCTACGCTCTAACGTACGTGAACTGGA'), 1.997696811857739),\n",
       " (Seq('GCGTCTACGCTCTAACGTACGTGAACTGGAA'), 1.997696811857739),\n",
       " (Seq('CGTCTACGCTCTAACGTACGTGAACTGGAAG'), 1.997696811857739),\n",
       " (Seq('TCTACGCTCTAACGTACGTGAACTGGAAGGC'), 1.997696811857739),\n",
       " (Seq('TTTACTGGCGTTGCAGGAAAAACTGGTCACC'), 1.997696811857739),\n",
       " (Seq('CATGCCTGTCGTAAAATTGAGCAACTGCGTG'), 1.997696811857739),\n",
       " (Seq('TTTATTAAAACCGCTTCAGCAGGTCAGCGGC'), 1.997696811857739),\n",
       " (Seq('TTCTCTGACCGGCACCGATCTTGAAATGGAG'), 1.997696811857739),\n",
       " (Seq('TCTCTGACCGGCACCGATCTTGAAATGGAGA'), 1.997696811857739),\n",
       " (Seq('CTCTGACCGGCACCGATCTTGAAATGGAGAT'), 1.997696811857739),\n",
       " (Seq('ACTACCGTGCCGGCGCGGAAATTCTTTGATA'), 1.997696811857739),\n",
       " (Seq('GATTTCCCGAATCTTGACGACTGGCAAAGCG'), 1.997696811857739),\n",
       " (Seq('AAGTTGAATTTACGCTGCCGCAGGCCACGAT'), 1.997696811857739),\n",
       " (Seq('TTGAATTTACGCTGCCGCAGGCCACGATGAA'), 1.997696811857739),\n",
       " (Seq('ATGAAGCGCCTGATTGAAGCGACCCAGTTTT'), 1.997696811857739),\n",
       " (Seq('TGAAGCGCCTGATTGAAGCGACCCAGTTTTC'), 1.997696811857739),\n",
       " (Seq('AAGCGCCTGATTGAAGCGACCCAGTTTTCGA'), 1.997696811857739),\n",
       " (Seq('AGCGCCTGATTGAAGCGACCCAGTTTTCGAT'), 1.997696811857739),\n",
       " (Seq('TGAAGCGACCCAGTTTTCGATGGCTCATCAG'), 1.997696811857739),\n",
       " (Seq('AAGCGACCCAGTTTTCGATGGCTCATCAGGA'), 1.997696811857739),\n",
       " (Seq('AGCGACCCAGTTTTCGATGGCTCATCAGGAT'), 1.997696811857739),\n",
       " (Seq('CTGCGCGTGCAGATCGGCAGTAATAATATCC'), 1.997696811857739),\n",
       " (Seq('TCGCGTTCTGCCGAAGAATCCGGATAAGCAT'), 1.997696811857739),\n",
       " (Seq('GCGTTCTGCCGAAGAATCCGGATAAGCATCT'), 1.997696811857739),\n",
       " (Seq('CGTTCTGCCGAAGAATCCGGATAAGCATCTG'), 1.997696811857739),\n",
       " (Seq('AATCCTCTCGAATGAGAAATTCCGCGGCGTG'), 1.997696811857739),\n",
       " (Seq('AAACCGTGCGCATTATGCTGACGGATTCCGT'), 1.997696811857739),\n",
       " (Seq('AACCGTGCGCATTATGCTGACGGATTCCGTA'), 1.997696811857739),\n",
       " (Seq('TATGCTGACGGATTCCGTATCCAGCGTACAG'), 1.997696811857739),\n",
       " (Seq('ATGCTGACGGATTCCGTATCCAGCGTACAGA'), 1.997696811857739),\n",
       " (Seq('TGCTGACGGATTCCGTATCCAGCGTACAGAT'), 1.997696811857739),\n",
       " (Seq('GCTGACGGATTCCGTATCCAGCGTACAGATT'), 1.997696811857739),\n",
       " (Seq('CTGACGGATTCCGTATCCAGCGTACAGATTG'), 1.997696811857739),\n",
       " (Seq('TGACGGATTCCGTATCCAGCGTACAGATTGA'), 1.997696811857739),\n",
       " (Seq('ATTCCGTATCCAGCGTACAGATTGAGGATGC'), 1.997696811857739),\n",
       " (Seq('GACAGTGCTCGAAATGCTCACGTACTTCGTG'), 1.997696811857739),\n",
       " (Seq('AACCAGCGTGCTGGAAGCTATCTATACGCTT'), 1.997696811857739),\n",
       " (Seq('ACCAGCGTGCTGGAAGCTATCTATACGCTTG'), 1.997696811857739),\n",
       " (Seq('TCCTTGACTGGGGATGCTTTCACAACGAAGC'), 1.997696811857739),\n",
       " (Seq('GGGATGCTTTCACAACGAAGCCGGATTCTTC'), 1.997696811857739),\n",
       " (Seq('GGATGCTTTCACAACGAAGCCGGATTCTTCA'), 1.997696811857739),\n",
       " (Seq('GCTGATTCCGTTGGCGGAACAAATTAGTACC'), 1.997696811857739),\n",
       " (Seq('ATTCCGTTGGCGGAACAAATTAGTACCTGGC'), 1.997696811857739),\n",
       " (Seq('ATACCCTGTCGCGCGGGCAGCTAAAGTTATT'), 1.997696811857739),\n",
       " (Seq('GCTTAAAAGCCACGCAATCGCAGGTCTTTGT'), 1.997696811857739),\n",
       " (Seq('ACGCAATCGCAGGTCTTTGTCAGCGCAATTA'), 1.997696811857739),\n",
       " (Seq('CGCAATCGCAGGTCTTTGTCAGCGCAATTAG'), 1.997696811857739),\n",
       " (Seq('GCAATCGCAGGTCTTTGTCAGCGCAATTAGC'), 1.997696811857739),\n",
       " (Seq('CAATCGCAGGTCTTTGTCAGCGCAATTAGCG'), 1.997696811857739),\n",
       " (Seq('AATCGCAGGTCTTTGTCAGCGCAATTAGCGC'), 1.997696811857739),\n",
       " (Seq('CAGGTCTTTGTCAGCGCAATTAGCGCTGAAC'), 1.997696811857739),\n",
       " (Seq('AGGTCTTTGTCAGCGCAATTAGCGCTGAACA'), 1.997696811857739),\n",
       " (Seq('GGTCTTTGTCAGCGCAATTAGCGCTGAACAC'), 1.997696811857739),\n",
       " (Seq('GTCTTTGTCAGCGCAATTAGCGCTGAACACG'), 1.997696811857739),\n",
       " (Seq('TCCTCCAGTATCAAAGTCCTGAAAGGGCTGG'), 1.997696811857739),\n",
       " (Seq('AGCGCTCGCAGGTCACTGTAAAGATATCGTC'), 1.997696811857739),\n",
       " (Seq('CGCTCGCAGGTCACTGTAAAGATATCGTCGT'), 1.997696811857739),\n",
       " (Seq('CTCGCAGGTCACTGTAAAGATATCGTCGTGA'), 1.997696811857739),\n",
       " (Seq('TCGCAGGTCACTGTAAAGATATCGTCGTGAC'), 1.997696811857739),\n",
       " (Seq('CGCAGGTCACTGTAAAGATATCGTCGTGACT'), 1.997696811857739),\n",
       " (Seq('CACGCGGGCGGTAAATTTGACGATAACTCCT'), 1.997696811857739),\n",
       " (Seq('CGCGGGCGGTAAATTTGACGATAACTCCTAT'), 1.997696811857739),\n",
       " (Seq('TTGACGATAACTCCTATAAAGTCTCCGGCGG'), 1.997696811857739),\n",
       " (Seq('TGACGATAACTCCTATAAAGTCTCCGGCGGT'), 1.997696811857739),\n",
       " (Seq('TCGGTAGTCAACGCTCTGTCGCAAAAACTGG'), 1.997696811857739),\n",
       " (Seq('CGCTCTGTCGCAAAAACTGGAACTGGTTATC'), 1.997696811857739),\n",
       " (Seq('GCTCTGTCGCAAAAACTGGAACTGGTTATCC'), 1.997696811857739),\n",
       " (Seq('CCGATAAAACCGGCACGATGGTACGTTTCTG'), 1.997696811857739),\n",
       " (Seq('ATAAAACCGGCACGATGGTACGTTTCTGGCC'), 1.997696811857739),\n",
       " (Seq('TTTGAATATGAGATCCTGGCGAAACGCCTGC'), 1.997696811857739),\n",
       " (Seq('ATTTCCACTACGAAGGCGGCATCAAGGCGTT'), 1.997696811857739),\n",
       " (Seq('TTTCCACTACGAAGGCGGCATCAAGGCGTTT'), 1.997696811857739),\n",
       " (Seq('GGCGATTCTGCCGCTGAAAGGTAAAATCCTC'), 1.997696811857739),\n",
       " (Seq('TTCGACAAGATGCTCTCCTCTCAGGAAGTGG'), 1.997696811857739),\n",
       " (Seq('CTTCTATCGTCAGATGCCGGAAATCGTCGAG'), 1.997696811857739),\n",
       " (Seq('TTCTATCGTCAGATGCCGGAAATCGTCGAGC'), 1.997696811857739),\n",
       " (Seq('AGCGATGGATCAGTACCAGATTTCCATCGCG'), 1.997696811857739),\n",
       " (Seq('GCTGCTCAAAGAGCTGGTGTATCAGCCAACT'), 1.997696811857739),\n",
       " (Seq('TGCTCAAAGAGCTGGTGTATCAGCCAACTCT'), 1.997696811857739),\n",
       " (Seq('GCTCAAAGAGCTGGTGTATCAGCCAACTCTG'), 1.997696811857739),\n",
       " (Seq('AACTCTGACCGAAGCCGATCTTTCTGATGAG'), 1.997696811857739),\n",
       " (Seq('TCTGACCGAAGCCGATCTTTCTGATGAGCAG'), 1.997696811857739),\n",
       " (Seq('CTGACCGAAGCCGATCTTTCTGATGAGCAGA'), 1.997696811857739),\n",
       " (Seq('TGACCGAAGCCGATCTTTCTGATGAGCAGAC'), 1.997696811857739),\n",
       " (Seq('GACCGAAGCCGATCTTTCTGATGAGCAGACT'), 1.997696811857739),\n",
       " (Seq('ACCGAAGCCGATCTTTCTGATGAGCAGACTG'), 1.997696811857739),\n",
       " (Seq('CCGAAGCCGATCTTTCTGATGAGCAGACTGT'), 1.997696811857739),\n",
       " (Seq('CGAAGCCGATCTTTCTGATGAGCAGACTGTA'), 1.997696811857739),\n",
       " (Seq('AGCCGATCTTTCTGATGAGCAGACTGTAACG'), 1.997696811857739),\n",
       " (Seq('GCCGATCTTTCTGATGAGCAGACTGTAACGC'), 1.997696811857739),\n",
       " (Seq('CCGATCTTTCTGATGAGCAGACTGTAACGCG'), 1.997696811857739),\n",
       " (Seq('CGATCTTTCTGATGAGCAGACTGTAACGCGC'), 1.997696811857739),\n",
       " (Seq('ACGGAACAGAATCTGTTCGAGCCTATCGTTC'), 1.997696811857739),\n",
       " (Seq('CGGAACAGAATCTGTTCGAGCCTATCGTTCG'), 1.997696811857739),\n",
       " (Seq('GGAACAGAATCTGTTCGAGCCTATCGTTCGC'), 1.997696811857739),\n",
       " (Seq('GAACAGAATCTGTTCGAGCCTATCGTTCGCG'), 1.997696811857739),\n",
       " (Seq('TGAAAGAATCACGTCGCGGTCTGGCTATCCA'), 1.997696811857739),\n",
       " (Seq('ACGTCGCGGTCTGGCTATCCAGCGTTATAAA'), 1.997696811857739),\n",
       " (Seq('TCAAAGATGCGATTGCTGCCGACCAGCTGTT'), 1.997696811857739),\n",
       " (Seq('AAAGATGCGATTGCTGCCGACCAGCTGTTCA'), 1.997696811857739),\n",
       " (Seq('CGTCGTGCCTTTATTGAAGAGAACGCCCTGA'), 1.997696811857739),\n",
       " (Seq('GTCGTGCCTTTATTGAAGAGAACGCCCTGAA'), 1.997696811857739),\n",
       " (Seq('CCGCGTAATCTCTCCCTTATATAAGGGAGAG'), 1.997696811857739),\n",
       " (Seq('TCCCAACATTTAACAGCGCCTGGAAGGTTTG'), 1.997696811857739),\n",
       " (Seq('CCCAACATTTAACAGCGCCTGGAAGGTTTGT'), 1.997696811857739),\n",
       " (Seq('CCAACATTTAACAGCGCCTGGAAGGTTTGTC'), 1.997696811857739),\n",
       " (Seq('ACATTTAACAGCGCCTGGAAGGTTTGTCAGC'), 1.997696811857739),\n",
       " (Seq('CATTTAACAGCGCCTGGAAGGTTTGTCAGCA'), 1.997696811857739),\n",
       " (Seq('ACCATTTCTCCGGCGGTTAAAAACGCGATTG'), 1.997696811857739),\n",
       " (Seq('ATTTCTCCGGCGGTTAAAAACGCGATTGCCG'), 1.997696811857739),\n",
       " (Seq('TTCACATGGAACAGCCCGGCGATTATTGCAT'), 1.997696811857739),\n",
       " (Seq('GCGATTATTGCATCACCTATAACGGGGCGCT'), 1.997696811857739),\n",
       " (Seq('CGATTATTGCATCACCTATAACGGGGCGCTG'), 1.997696811857739),\n",
       " (Seq('TTGCGCAAACGGCGCTCAGCTATGATGACTA'), 1.997696811857739),\n",
       " (Seq('GCTCAGCTATGATGACTACCGTTACCTGGAA'), 1.997696811857739),\n",
       " (Seq('TACCGTTACCTGGAAAAACTGTCCCGTGAGG'), 1.997696811857739),\n",
       " (Seq('ACCGTTACCTGGAAAAACTGTCCCGTGAGGT'), 1.997696811857739),\n",
       " (Seq('CGCGATATCAGCTACTACACGGTGCATGAGT'), 1.997696811857739),\n",
       " (Seq('GCGATATCAGCTACTACACGGTGCATGAGTC'), 1.997696811857739),\n",
       " (Seq('CGATATCAGCTACTACACGGTGCATGAGTCG'), 1.997696811857739),\n",
       " (Seq('GATATCAGCTACTACACGGTGCATGAGTCGT'), 1.997696811857739),\n",
       " (Seq('ATCAGCTACTACACGGTGCATGAGTCGTATG'), 1.997696811857739),\n",
       " (Seq('CAGCTACTACACGGTGCATGAGTCGTATGTA'), 1.997696811857739),\n",
       " (Seq('CCTGAAAGTTATGATGATCGATGAGCCTGCC'), 1.997696811857739),\n",
       " (Seq('AGTACACCGTGCTGAAAAGTGCGCCGTACTT'), 1.997696811857739),\n",
       " (Seq('CGTGCTGAAAAGTGCGCCGTACTTCCTTGAA'), 1.997696811857739),\n",
       " (Seq('GTGCTGAAAAGTGCGCCGTACTTCCTTGAAA'), 1.997696811857739),\n",
       " (Seq('GCTGAAAAGTGCGCCGTACTTCCTTGAAATC'), 1.997696811857739),\n",
       " (Seq('CCATTCCGTCGGTCAAAGAGGTGGCTAACTT'), 1.997696811857739),\n",
       " (Seq('GGGCCGATAGTTCCACCCATGAAGCTAATGT'), 1.997696811857739),\n",
       " (Seq('GGCCGATAGTTCCACCCATGAAGCTAATGTG'), 1.997696811857739),\n",
       " (Seq('GCCGATAGTTCCACCCATGAAGCTAATGTGT'), 1.997696811857739),\n",
       " (Seq('AAAGCGTGGCTATGAGCTTCCCGAGCCATTT'), 1.997696811857739),\n",
       " (Seq('CTTTAGGTCCCAACGGGGGAATTCCATTAGA'), 1.997696811857739),\n",
       " (Seq('TCGACTTTAAAGGCACTACCTATGGCATCGG'), 1.997696811857739),\n",
       " (Seq('CCCAGACACAGTTCGATATTCTTGATGGCAG'), 1.997696811857739),\n",
       " (Seq('CCAGACACAGTTCGATATTCTTGATGGCAGC'), 1.997696811857739),\n",
       " (Seq('CCGTTTTACCACCCCGGGTATAGATAAGATG'), 1.997696811857739),\n",
       " (Seq('CGTTTTACCACCCCGGGTATAGATAAGATGC'), 1.997696811857739),\n",
       " (Seq('ACCCCGGGTATAGATAAGATGCATCTGCCGT'), 1.997696811857739),\n",
       " (Seq('ATCTACAATCACCGTGGAACATGCTGCTGGG'), 1.997696811857739),\n",
       " (Seq('CATGCTGCTGGGCGCTCAGTATGAAATAACC'), 1.997696811857739),\n",
       " (Seq('ATGCTGCTGGGCGCTCAGTATGAAATAACCC'), 1.997696811857739),\n",
       " (Seq('TGGGCGCTCAGTATGAAATAACCCGCCATTT'), 1.997696811857739),\n",
       " (Seq('GATCCTGCCGATAAGGTTAGCCAGGTATCCA'), 1.997696811857739),\n",
       " (Seq('ATAAGGTTAGCCAGGTATCCACGCTGTCGCT'), 1.997696811857739),\n",
       " (Seq('CACAGGCGCTTTTGGCGTAAGCCTGCAAAAT'), 1.997696811857739),\n",
       " (Seq('ACAGGCGCTTTTGGCGTAAGCCTGCAAAATT'), 1.997696811857739),\n",
       " (Seq('CAGGCGCTTTTGGCGTAAGCCTGCAAAATTA'), 1.997696811857739),\n",
       " (Seq('AGGCGCTTTTGGCGTAAGCCTGCAAAATTAC'), 1.997696811857739),\n",
       " (Seq('GCGCTTTTGGCGTAAGCCTGCAAAATTACGC'), 1.997696811857739),\n",
       " (Seq('ATTGAGCGACACGCCCACTTACTATTGGGGA'), 1.997696811857739),\n",
       " (Seq('ACACGCCCACTTACTATTGGGGACAGGGTTT'), 1.997696811857739),\n",
       " (Seq('ATTCAAAGTACAGCGCAGGGCTGTTCTTCAC'), 1.997696811857739),\n",
       " (Seq('TCAAAGTACAGCGCAGGGCTGTTCTTCACTG'), 1.997696811857739),\n",
       " (Seq('CAAAGTACAGCGCAGGGCTGTTCTTCACTGA'), 1.997696811857739),\n",
       " (Seq('TACAGCGCAGGGCTGTTCTTCACTGAGTAAC'), 1.997696811857739),\n",
       " (Seq('CATGCCCTCAATGATAAAGATGTGCTGGCCT'), 1.997696811857739),\n",
       " (Seq('ATGCCCTCAATGATAAAGATGTGCTGGCCTG'), 1.997696811857739),\n",
       " (Seq('GCAGTAACCATCGTATGCGCGGCTATTATGA'), 1.997696811857739),\n",
       " (Seq('AATGCTATCAGCGGTCAGCTGGAATATCGCC'), 1.997696811857739),\n",
       " (Seq('ATGCTATCAGCGGTCAGCTGGAATATCGCCA'), 1.997696811857739),\n",
       " (Seq('TGCTATCAGCGGTCAGCTGGAATATCGCCAT'), 1.997696811857739),\n",
       " (Seq('GCTATCAGCGGTCAGCTGGAATATCGCCATA'), 1.997696811857739),\n",
       " (Seq('TCAGCGGTCAGCTGGAATATCGCCATAAGCT'), 1.997696811857739),\n",
       " (Seq('ATCGTCTTTGCACCATCTGGATGACGGACGA'), 1.997696811857739),\n",
       " (Seq('ACTACTACCAAAGCTGGGGCAGGCTTTTACG'), 1.997696811857739),\n",
       " (Seq('TACTACCAAAGCTGGGGCAGGCTTTTACGCT'), 1.997696811857739),\n",
       " (Seq('CACGAAATCGCCCAATGGTACGGTTTCGAGT'), 1.997696811857739),\n",
       " (Seq('ACGAAATCGCCCAATGGTACGGTTTCGAGTC'), 1.997696811857739),\n",
       " (Seq('CGAAATCGCCCAATGGTACGGTTTCGAGTCT'), 1.997696811857739),\n",
       " (Seq('CGTCTTTAATTGTCGGCGGCCAGACGAAAAC'), 1.997696811857739),\n",
       " (Seq('TCTTTAATTGTCGGCGGCCAGACGAAAACGC'), 1.997696811857739),\n",
       " (Seq('CTGCATCGTAATTATCAGATGGGCGATGACC'), 1.997696811857739),\n",
       " (Seq('CATCGTAATTATCAGATGGGCGATGACCGCC'), 1.997696811857739),\n",
       " (Seq('ATCGTAATTATCAGATGGGCGATGACCGCCT'), 1.997696811857739),\n",
       " (Seq('AGCTTAGCACGCTGGCGCAACTACAATTGTG'), 1.997696811857739),\n",
       " (Seq('TTACAGCGAAAAAGATTTTGCCGCCCTGGCG'), 1.997696811857739),\n",
       " (Seq('TTAAACAGGCAGTAGCCGTAGGCTATTTCCC'), 1.997696811857739),\n",
       " (Seq('TAAACAGGCAGTAGCCGTAGGCTATTTCCCT'), 1.997696811857739),\n",
       " (Seq('CTCGACTAAAGGCAGCGCGCTGTCTTTGAAT'), 1.997696811857739),\n",
       " (Seq('TCGACTAAAGGCAGCGCGCTGTCTTTGAATA'), 1.997696811857739),\n",
       " (Seq('CTAAAGGCAGCGCGCTGTCTTTGAATAACGC'), 1.997696811857739),\n",
       " (Seq('TGTCTTTGAATAACGCGACCCATTGCCGGAG'), 1.997696811857739),\n",
       " (Seq('GTCTTTGAATAACGCGACCCATTGCCGGAGT'), 1.997696811857739),\n",
       " (Seq('ATATCGCCGGCAAGCTGATGTTCATTCAGCA'), 1.997696811857739),\n",
       " (Seq('GCCCTGTAAATAGTGCGCTATCATGCGGTAA'), 1.997696811857739),\n",
       " (Seq('AATAGTGCGCTATCATGCGGTAAAGCTCCTC'), 1.997696811857739),\n",
       " (Seq('ATAGTGCGCTATCATGCGGTAAAGCTCCTCG'), 1.997696811857739),\n",
       " (Seq('GCGGTACGCTGACGAAGATTATCGTCTATCA'), 1.997696811857739),\n",
       " (Seq('CGGTACGCTGACGAAGATTATCGTCTATCAC'), 1.997696811857739),\n",
       " (Seq('CGCTGACGAAGATTATCGTCTATCACATGCG'), 1.997696811857739),\n",
       " (Seq('GCTGACGAAGATTATCGTCTATCACATGCGC'), 1.997696811857739),\n",
       " (Seq('CTGACGAAGATTATCGTCTATCACATGCGCG'), 1.997696811857739),\n",
       " (Seq('TGACGAAGATTATCGTCTATCACATGCGCGC'), 1.997696811857739),\n",
       " (Seq('GACGAAGATTATCGTCTATCACATGCGCGCT'), 1.997696811857739),\n",
       " (Seq('ACATGCGCGCTAAAACCGATGCGTTTCATGG'), 1.997696811857739),\n",
       " (Seq('CTAAAACCGATGCGTTTCATGGCGGGATACT'), 1.997696811857739),\n",
       " (Seq('GCGTTTCATGGCGGGATACTGACTCATCAAC'), 1.997696811857739),\n",
       " (Seq('TCATGGCGGGATACTGACTCATCAACTGCTG'), 1.997696811857739),\n",
       " (Seq('AACTGCTGCGCCAGGGTTAAGCCGTCATAAT'), 1.997696811857739),\n",
       " (Seq('TACCCGTGAGCATTTCAGCGGTAATACGCTG'), 1.997696811857739),\n",
       " (Seq('AGCATTTCAGCGGTAATACGCTGCGTCAGCA'), 1.997696811857739),\n",
       " (Seq('GCATTTCAGCGGTAATACGCTGCGTCAGCAT'), 1.997696811857739),\n",
       " (Seq('CATTTCAGCGGTAATACGCTGCGTCAGCATA'), 1.997696811857739),\n",
       " (Seq('CAGCGGTAATACGCTGCGTCAGCATATTATC'), 1.997696811857739),\n",
       " (Seq('AAATAGCCGTCAGCTTTGCTTCAGGGATACC'), 1.997696811857739),\n",
       " (Seq('AATAGCCGTCAGCTTTGCTTCAGGGATACCG'), 1.997696811857739),\n",
       " (Seq('GCAGCACTATGCTGCCGCGATCGGTAAATTT'), 1.997696811857739),\n",
       " (Seq('AGCACTATGCTGCCGCGATCGGTAAATTTCG'), 1.997696811857739),\n",
       " (Seq('GATTAATCACAATTTGACGTATCCGGCGCGG'), 1.997696811857739),\n",
       " (Seq('TAATCACAATTTGACGTATCCGGCGCGGATC'), 1.997696811857739),\n",
       " (Seq('AATCACAATTTGACGTATCCGGCGCGGATCG'), 1.997696811857739),\n",
       " (Seq('CGGCTGTGCATTAAATGCAGCGCGCTATTCA'), 1.997696811857739),\n",
       " (Seq('GGCTGTGCATTAAATGCAGCGCGCTATTCAA'), 1.997696811857739),\n",
       " (Seq('GCTGTGCATTAAATGCAGCGCGCTATTCAAC'), 1.997696811857739),\n",
       " (Seq('AATGCAGCGCGCTATTCAACAATTGTCGCGG'), 1.997696811857739),\n",
       " (Seq('ATGCAGCGCGCTATTCAACAATTGTCGCGGT'), 1.997696811857739),\n",
       " (Seq('AGCGCGCTATTCAACAATTGTCGCGGTTCAA'), 1.997696811857739),\n",
       " (Seq('GCGCGCTATTCAACAATTGTCGCGGTTCAAA'), 1.997696811857739),\n",
       " (Seq('GCGCTATTCAACAATTGTCGCGGTTCAAACG'), 1.997696811857739),\n",
       " (Seq('CGCTATTCAACAATTGTCGCGGTTCAAACGG'), 1.997696811857739),\n",
       " (Seq('GCTATTCAACAATTGTCGCGGTTCAAACGGC'), 1.997696811857739),\n",
       " (Seq('AACGGCTCCTCGCTAATAGAAACGTTGGTGC'), 1.997696811857739),\n",
       " (Seq('GCTAATAGAAACGTTGGTGCCGCCTACTTCA'), 1.997696811857739),\n",
       " (Seq('ATAGAAACGTTGGTGCCGCCTACTTCAATCG'), 1.997696811857739),\n",
       " (Seq('TATCGTTAAGAATCGCCAGTAGCGATTCGCC'), 1.997696811857739),\n",
       " (Seq('ATCGTTAAGAATCGCCAGTAGCGATTCGCCT'), 1.997696811857739),\n",
       " (Seq('TCGTTAAGAATCGCCAGTAGCGATTCGCCTG'), 1.997696811857739),\n",
       " (Seq('CGTTAAGAATCGCCAGTAGCGATTCGCCTGA'), 1.997696811857739),\n",
       " (Seq('GAATCGCCAGTAGCGATTCGCCTGAATCATT'), 1.997696811857739),\n",
       " (Seq('CGCCAGTAGCGATTCGCCTGAATCATTAATG'), 1.997696811857739),\n",
       " (Seq('GCCAGTAGCGATTCGCCTGAATCATTAATGG'), 1.997696811857739),\n",
       " (Seq('CCAGTAGCGATTCGCCTGAATCATTAATGGC'), 1.997696811857739),\n",
       " (Seq('CAGTAGCGATTCGCCTGAATCATTAATGGCC'), 1.997696811857739),\n",
       " (Seq('CCATGAGCGGTTTATCGGCCAGCAATTGTAC'), 1.997696811857739),\n",
       " (Seq('CATGAGCGGTTTATCGGCCAGCAATTGTACC'), 1.997696811857739),\n",
       " (Seq('AGCGGTTTATCGGCCAGCAATTGTACCGTAC'), 1.997696811857739),\n",
       " (Seq('GGTTTATCGGCCAGCAATTGTACCGTACCGA'), 1.997696811857739),\n",
       " (Seq('GTTTATCGGCCAGCAATTGTACCGTACCGAG'), 1.997696811857739),\n",
       " (Seq('TTTATCGGCCAGCAATTGTACCGTACCGAGA'), 1.997696811857739),\n",
       " (Seq('AAATCCTCACGATGGCGGTTCAATTTGCGCA'), 1.997696811857739),\n",
       " (Seq('CGAAACGCTTCCATCAGACGGCTGATGGTAT'), 1.997696811857739),\n",
       " (Seq('GAAACGCTTCCATCAGACGGCTGATGGTATC'), 1.997696811857739),\n",
       " (Seq('TGCGACACTTCCGTACTGAAGCGGGTAAACT'), 1.997696811857739),\n",
       " (Seq('GATGGCGTTTTCCTTACGAAACAGGGTAACC'), 1.997696811857739),\n",
       " (Seq('TGGCGTTTTCCTTACGAAACAGGGTAACCAG'), 1.997696811857739),\n",
       " (Seq('GGCGTTTTCCTTACGAAACAGGGTAACCAGT'), 1.997696811857739),\n",
       " (Seq('GCGTTTTCCTTACGAAACAGGGTAACCAGTG'), 1.997696811857739),\n",
       " (Seq('GTCGATATCAATCAATCGGTCAAGCGCGCGT'), 1.997696811857739),\n",
       " (Seq('TCGATATCAATCAATCGGTCAAGCGCGCGTT'), 1.997696811857739),\n",
       " (Seq('CGCTTTCGATAAGATCGTAAATACCGGCCTG'), 1.997696811857739),\n",
       " (Seq('GCTTTCGATAAGATCGTAAATACCGGCCTGG'), 1.997696811857739),\n",
       " (Seq('CTTTCGATAAGATCGTAAATACCGGCCTGGG'), 1.997696811857739),\n",
       " (Seq('AAAGAGCTCGTAAGCGCTGGCTTCGCTTAAC'), 1.997696811857739),\n",
       " (Seq('AAGAGCTCGTAAGCGCTGGCTTCGCTTAACT'), 1.997696811857739),\n",
       " (Seq('TCATCGTCTCGAATGTAGAAACGGCGTCAGC'), 1.997696811857739),\n",
       " (Seq('TACTGGTTGTCCGTCAACTACGGGATGCAAA'), 1.997696811857739),\n",
       " (Seq('TACCGATCGTTTCCCGGAACTGGAACGGTAT'), 1.997696811857739),\n",
       " (Seq('ACTGGTGAACATGATTCACGATGCCAGCGTC'), 1.997696811857739),\n",
       " (Seq('TAACGTTTTACTCTTCCCGGGGCCGGAAGAA'), 1.997696811857739),\n",
       " (Seq('GTTTTCGTCAGGCAATTAAAGGCAGCGCCAT'), 1.997696811857739),\n",
       " (Seq('TTTTCGTCAGGCAATTAAAGGCAGCGCCATC'), 1.997696811857739),\n",
       " (Seq('GACGCATCAGGTTTATCGCGGTTTAAAACGC'), 1.997696811857739),\n",
       " (Seq('ACGCATCAGGTTTATCGCGGTTTAAAACGCG'), 1.997696811857739),\n",
       " (Seq('CATCAGGTTTATCGCGGTTTAAAACGCGGCC'), 1.997696811857739),\n",
       " (Seq('ATCAGGTTTATCGCGGTTTAAAACGCGGCCA'), 1.997696811857739),\n",
       " (Seq('CAGGTTTATCGCGGTTTAAAACGCGGCCATA'), 1.997696811857739),\n",
       " (Seq('TATCGCGGTTTAAAACGCGGCCATATCCTGA'), 1.997696811857739),\n",
       " (Seq('ATCGCGGTTTAAAACGCGGCCATATCCTGAT'), 1.997696811857739),\n",
       " (Seq('TCGCGGTTTAAAACGCGGCCATATCCTGATG'), 1.997696811857739),\n",
       " (Seq('GTTTAAAACGCGGCCATATCCTGATGGCGCT'), 1.997696811857739),\n",
       " (Seq('CGAGGCTAAAAAGTAGCCTTCGCCATGCTGT'), 1.997696811857739),\n",
       " (Seq('AGGCTAAAAAGTAGCCTTCGCCATGCTGTGT'), 1.997696811857739),\n",
       " (Seq('GCTAAAAAGTAGCCTTCGCCATGCTGTGTGA'), 1.997696811857739),\n",
       " (Seq('CTAAAAAGTAGCCTTCGCCATGCTGTGTGAC'), 1.997696811857739),\n",
       " (Seq('TAAAAAGTAGCCTTCGCCATGCTGTGTGACC'), 1.997696811857739),\n",
       " (Seq('AAAAGTAGCCTTCGCCATGCTGTGTGACCAG'), 1.997696811857739),\n",
       " (Seq('TTGGTCACAAAGGCCAGCAGTAGCTCGTACT'), 1.997696811857739),\n",
       " (Seq('CCAGCGTGTGATTCATCACGTTCAGGCAGTA'), 1.997696811857739),\n",
       " (Seq('AGAATAATGCCCACCGTGGAGCGTTCTCGTA'), 1.997696811857739),\n",
       " (Seq('GGATCAGCGAAACGTGCTCATGCTCCATGAT'), 1.997696811857739),\n",
       " (Seq('GATCAGCGAAACGTGCTCATGCTCCATGATG'), 1.997696811857739),\n",
       " (Seq('ATCAGCGAAACGTGCTCATGCTCCATGATGT'), 1.997696811857739),\n",
       " (Seq('CTGCTCAAAATAGGCCTGTAATCTGGCCTGA'), 1.997696811857739),\n",
       " (Seq('TGCTCAAAATAGGCCTGTAATCTGGCCTGAG'), 1.997696811857739),\n",
       " (Seq('GCTCAAAATAGGCCTGTAATCTGGCCTGAGT'), 1.997696811857739),\n",
       " (Seq('ACTGACCAGTACGACAGAGTTTTGCGTCAGC'), 1.997696811857739),\n",
       " (Seq('CTGACCAGTACGACAGAGTTTTGCGTCAGCT'), 1.997696811857739),\n",
       " (Seq('CAGTACGACAGAGTTTTGCGTCAGCTGCCAT'), 1.997696811857739),\n",
       " (Seq('AGTACGACAGAGTTTTGCGTCAGCTGCCATA'), 1.997696811857739),\n",
       " (Seq('ACGACAGAGTTTTGCGTCAGCTGCCATAGTA'), 1.997696811857739),\n",
       " (Seq('TCAGCTGCCATAGTATGCAGCCGGTGTATCA'), 1.997696811857739),\n",
       " (Seq('GTCGTTCCTGCCATAACTACGATGCGATGGA'), 1.997696811857739),\n",
       " (Seq('GTTCCTGCCATAACTACGATGCGATGGATCA'), 1.997696811857739),\n",
       " (Seq('CTACCGGATATGAGCAGCGGTTTTCGCAAAC'), 1.997696811857739),\n",
       " (Seq('TTTCGCAAACAGTTTGATGAACTGCGCGCCA'), 1.997696811857739),\n",
       " (Seq('TTCGCAAACAGTTTGATGAACTGCGCGCCAG'), 1.997696811857739),\n",
       " (Seq('GTACGCATAATGACGGCGATACGCTCTATTC'), 1.997696811857739),\n",
       " (Seq('TACGCATAATGACGGCGATACGCTCTATTCG'), 1.997696811857739),\n",
       " (Seq('GCATAATGACGGCGATACGCTCTATTCGCTG'), 1.997696811857739),\n",
       " (Seq('CATAATGACGGCGATACGCTCTATTCGCTGG'), 1.997696811857739),\n",
       " (Seq('ATAATGACGGCGATACGCTCTATTCGCTGGA'), 1.997696811857739),\n",
       " (Seq('AATGACGGCGATACGCTCTATTCGCTGGATA'), 1.997696811857739),\n",
       " (Seq('GACGGCGATACGCTCTATTCGCTGGATATCA'), 1.997696811857739),\n",
       " (Seq('ACGGCGATACGCTCTATTCGCTGGATATCAA'), 1.997696811857739),\n",
       " (Seq('CGGCGATACGCTCTATTCGCTGGATATCAAG'), 1.997696811857739),\n",
       " (Seq('GGCGATACGCTCTATTCGCTGGATATCAAGC'), 1.997696811857739),\n",
       " (Seq('GATACGCTCTATTCGCTGGATATCAAGCCGA'), 1.997696811857739),\n",
       " (Seq('CGATAAAGAACCGGCAGGTTCGCTGTTACCT'), 1.997696811857739),\n",
       " (Seq('ATAAAGAACCGGCAGGTTCGCTGTTACCTGC'), 1.997696811857739),\n",
       " (Seq('TAAAGAACCGGCAGGTTCGCTGTTACCTGCT'), 1.997696811857739),\n",
       " (Seq('AAAGAACCGGCAGGTTCGCTGTTACCTGCTT'), 1.997696811857739),\n",
       " (Seq('CCTGCTTCTGAAGTGAAAGTCCTTAAACGGG'), 1.997696811857739),\n",
       " (Seq('TGACATTAAACCGATTTGGGCCTATGCCGAC'), 1.997696811857739),\n",
       " (Seq('GACATTAAACCGATTTGGGCCTATGCCGACT'), 1.997696811857739),\n",
       " (Seq('TCTATAACGGCACCTGTAATCAGTGTCACGG'), 1.997696811857739),\n",
       " (Seq('TATAACGGCACCTGTAATCAGTGTCACGGCG'), 1.997696811857739),\n",
       " (Seq('TCAACGGCATGATCGGTTTCACCAGTCTGGA'), 1.997696811857739),\n",
       " (Seq('CAACGGCATGATCGGTTTCACCAGTCTGGAT'), 1.997696811857739),\n",
       " (Seq('AACGGCATGATCGGTTTCACCAGTCTGGATA'), 1.997696811857739),\n",
       " (Seq('ACGGCATGATCGGTTTCACCAGTCTGGATAA'), 1.997696811857739),\n",
       " (Seq('TACGAGCAGCAAACGTCATGGCCTCTGGTTT'), 1.997696811857739),\n",
       " (Seq('ACGAGCAGCAAACGTCATGGCCTCTGGTTTT'), 1.997696811857739),\n",
       " (Seq('CGAGCAGCAAACGTCATGGCCTCTGGTTTTA'), 1.997696811857739),\n",
       " (Seq('GAGCAGCAAACGTCATGGCCTCTGGTTTTAC'), 1.997696811857739),\n",
       " (Seq('AGCAGCAAACGTCATGGCCTCTGGTTTTACA'), 1.997696811857739),\n",
       " (Seq('GCAGCAAACGTCATGGCCTCTGGTTTTACAG'), 1.997696811857739),\n",
       " (Seq('CAGCAAACGTCATGGCCTCTGGTTTTACAGA'), 1.997696811857739),\n",
       " (Seq('GCAAACGTCATGGCCTCTGGTTTTACAGAAC'), 1.997696811857739),\n",
       " (Seq('GGTGCCCGGATCACGATGTCTATCAGTACTA'), 1.997696811857739),\n",
       " (Seq('GCCCGGATCACGATGTCTATCAGTACTATGA'), 1.997696811857739),\n",
       " (Seq('AGTTCCTCCCCTATTTGCTGGGCGAAAAAGA'), 1.997696811857739),\n",
       " (Seq('TCCTCCCCTATTTGCTGGGCGAAAAAGATGG'), 1.997696811857739),\n",
       " (Seq('CCTCCCCTATTTGCTGGGCGAAAAAGATGGA'), 1.997696811857739),\n",
       " (Seq('CTCCCCTATTTGCTGGGCGAAAAAGATGGAC'), 1.997696811857739),\n",
       " (Seq('CAAATCATCGCTGGCTGGTGCGTACAACGTA'), 1.997696811857739),\n",
       " (Seq('AAATCATCGCTGGCTGGTGCGTACAACGTAT'), 1.997696811857739),\n",
       " (Seq('GATGCCATTCTTGAACCGGGCAAGGTCATTA'), 1.997696811857739),\n",
       " (Seq('TGCCATTCTTGAACCGGGCAAGGTCATTAAC'), 1.997696811857739),\n",
       " (Seq('GCCATTCTTGAACCGGGCAAGGTCATTAACT'), 1.997696811857739),\n",
       " (Seq('CCATTCTTGAACCGGGCAAGGTCATTAACTG'), 1.997696811857739),\n",
       " (Seq('CATTCTTGAACCGGGCAAGGTCATTAACTGG'), 1.997696811857739),\n",
       " (Seq('ATCGGTTAAATTACCGCCGCTGAAGATGTGC'), 1.997696811857739),\n",
       " (Seq('ACGGTCATCGCCATTGATAACCAGTGGACGT'), 1.997696811857739),\n",
       " (Seq('GTCATCGCCATTGATAACCAGTGGACGTCGA'), 1.997696811857739),\n",
       " (Seq('CAATTTGAGCGTAACGACCTCGATCAGTTCG'), 1.997696811857739),\n",
       " (Seq('AATTTGAGCGTAACGACCTCGATCAGTTCGG'), 1.997696811857739),\n",
       " (Seq('ATTTGAGCGTAACGACCTCGATCAGTTCGGC'), 1.997696811857739),\n",
       " (Seq('TTTGAGCGTAACGACCTCGATCAGTTCGGCA'), 1.997696811857739),\n",
       " (Seq('TTGAGCGTAACGACCTCGATCAGTTCGGCAA'), 1.997696811857739),\n",
       " (Seq('CCGCAGTTTGAAGCGCGTAACGACTTTGATA'), 1.997696811857739),\n",
       " (Seq('GGAAAAGGTCGCGGTATCCACTTACCGACTT'), 1.997696811857739),\n",
       " (Seq('GAAAAGGTCGCGGTATCCACTTACCGACTTT'), 1.997696811857739),\n",
       " (Seq('ACCGACTTTCGAGGTGTTCTGGAATCAACAG'), 1.997696811857739),\n",
       " (Seq('GCACGCCAAGCGGTTTGATCGAGATTTACTC'), 1.997696811857739),\n",
       " (Seq('ACGCCAAGCGGTTTGATCGAGATTTACTCCA'), 1.997696811857739),\n",
       " (Seq('CGCCAAGCGGTTTGATCGAGATTTACTCCAA'), 1.997696811857739),\n",
       " (Seq('AATACGACGATTGTCAGGGCCATCCCATGTG'), 1.997696811857739),\n",
       " (Seq('ATTGTCAGGGCCATCCCATGTGGTTCGAAAA'), 1.997696811857739),\n",
       " (Seq('TTGTCAGGGCCATCCCATGTGGTTCGAAAAA'), 1.997696811857739),\n",
       " (Seq('AGGAGCCGGTCTTTATTAACCCACAGGATGC'), 1.997696811857739),\n",
       " (Seq('AATTCATGAAGGCGCCTGGTACGATCCTGAT'), 1.997696811857739),\n",
       " (Seq('ATTCATGAAGGCGCCTGGTACGATCCTGATA'), 1.997696811857739),\n",
       " (Seq('TTCATGAAGGCGCCTGGTACGATCCTGATAA'), 1.997696811857739),\n",
       " (Seq('ATCCTGATAAAGGCGGCGATCTCAATGCGTT'), 1.997696811857739),\n",
       " (Seq('TCCTGATAAAGGCGGCGATCTCAATGCGTTA'), 1.997696811857739),\n",
       " (Seq('CCTGATAAAGGCGGCGATCTCAATGCGTTAT'), 1.997696811857739),\n",
       " (Seq('TTAACGCTGGATATCGGTACATCACAGCTCG'), 1.997696811857739),\n",
       " (Seq('GGCCCTATTGATAACGTCACAGCGTTTAACG'), 1.997696811857739),\n",
       " (Seq('GCCCTATTGATAACGTCACAGCGTTTAACGG'), 1.997696811857739),\n",
       " (Seq('ATTGATAACGTCACAGCGTTTAACGGTCCGG'), 1.997696811857739),\n",
       " (Seq('TGATAACGTCACAGCGTTTAACGGTCCGGTC'), 1.997696811857739),\n",
       " (Seq('ATAACGTCACAGCGTTTAACGGTCCGGTCGA'), 1.997696811857739),\n",
       " (Seq('GCTTGCTCAGGAACAGTACGCCTGCGTATAT'), 1.997696811857739),\n",
       " (Seq('CTTGCTCAGGAACAGTACGCCTGCGTATATG'), 1.997696811857739),\n",
       " (Seq('TTGCTCAGGAACAGTACGCCTGCGTATATGC'), 1.997696811857739),\n",
       " (Seq('TGCTCAGGAACAGTACGCCTGCGTATATGCA'), 1.997696811857739),\n",
       " (Seq('GCTCAGGAACAGTACGCCTGCGTATATGCAT'), 1.997696811857739),\n",
       " (Seq('CTCAGGAACAGTACGCCTGCGTATATGCATG'), 1.997696811857739),\n",
       " (Seq('GGCCGACCACCTGGCGATTTATCTGGAATTA'), 1.997696811857739),\n",
       " (Seq('CCTCTTTTCCGGTGGCAAAGCAGCTAATGGA'), 1.997696811857739),\n",
       " (Seq('GCAGCTAATGGATTACGATATCCAGCTCGGC'), 1.997696811857739),\n",
       " (Seq('CAGCTAATGGATTACGATATCCAGCTCGGCT'), 1.997696811857739),\n",
       " (Seq('GCTAATGGATTACGATATCCAGCTCGGCTAC'), 1.997696811857739),\n",
       " (Seq('CAGCTCGGCTACAAATCGTTTAACCTGGAGG'), 1.997696811857739),\n",
       " (Seq('AGCTCGGCTACAAATCGTTTAACCTGGAGGC'), 1.997696811857739),\n",
       " (Seq('GCGTACTGACATCCTGAACTGGATAGCCGAT'), 1.997696811857739),\n",
       " (Seq('TGACATCCTGAACTGGATAGCCGATCAGCGT'), 1.997696811857739),\n",
       " (Seq('GCGGACCGCCGTTAAATCCTATTGAAATGGC'), 1.997696811857739),\n",
       " (Seq('GGACCGCCGTTAAATCCTATTGAAATGGCCT'), 1.997696811857739),\n",
       " (Seq('GTTGATAACGCCGGACTCCGCCTTTGATAAA'), 1.997696811857739),\n",
       " (Seq('TGATAACGCCGGACTCCGCCTTTGATAAATG'), 1.997696811857739),\n",
       " (Seq('GATAACGCCGGACTCCGCCTTTGATAAATGG'), 1.997696811857739),\n",
       " (Seq('ACTCCGCCTTTGATAAATGGCTCCGTGGAGA'), 1.997696811857739),\n",
       " (Seq('TAAAACTGATGCTGCGTTACCAGGTTGGCAC'), 1.997696811857739),\n",
       " (Seq('AAAACTGATGCTGCGTTACCAGGTTGGCACC'), 1.997696811857739),\n",
       " (Seq('GGTTGGCACCGATCTGCCGCAAAACGATATT'), 1.997696811857739),\n",
       " (Seq('GTTGGCACCGATCTGCCGCAAAACGATATTG'), 1.997696811857739),\n",
       " (Seq('TCTGCCGCAAAACGATATTGACGATGTCGTC'), 1.997696811857739),\n",
       " (Seq('CTGCCGCAAAACGATATTGACGATGTCGTCG'), 1.997696811857739),\n",
       " (Seq('TGCCGCAAAACGATATTGACGATGTCGTCGC'), 1.997696811857739),\n",
       " (Seq('CGCAAAACGATATTGACGATGTCGTCGCCTT'), 1.997696811857739),\n",
       " (Seq('GCAAAACGATATTGACGATGTCGTCGCCTTC'), 1.997696811857739),\n",
       " (Seq('CTGGAAAGCCTGACCGGTGTTTATACGCCAT'), 1.997696811857739),\n",
       " (Seq('TGGAAAGCCTGACCGGTGTTTATACGCCATA'), 1.997696811857739),\n",
       " (Seq('GGAAAGCCTGACCGGTGTTTATACGCCATAT'), 1.997696811857739),\n",
       " (Seq('GAAAGCCTGACCGGTGTTTATACGCCATATC'), 1.997696811857739),\n",
       " (Seq('GACTATGTGGAAAACCCTTCATCAGCTGGCG'), 1.997696811857739),\n",
       " (Seq('ACTATGTGGAAAACCCTTCATCAGCTGGCGG'), 1.997696811857739),\n",
       " (Seq('CTGATTTTACTGATGGAAAAACGCCGCCCGT'), 1.997696811857739),\n",
       " (Seq('TGATTTTACTGATGGAAAAACGCCGCCCGTG'), 1.997696811857739),\n",
       " (Seq('CGAAAACCTCTCTCAGCTACTGGGGAATGGT'), 1.997696811857739),\n",
       " (Seq('ACTACAAACCGTTTGTCCGCTGGATATGGGC'), 1.997696811857739),\n",
       " (Seq('GAAACGCAACGTACTGTTATTACCGCTGCTG'), 1.997696811857739),\n",
       " (Seq('AGTACCTGAACCAGCTTTCTGCGCAGGGTAT'), 1.997696811857739),\n",
       " (Seq('TAACCCGTATGCGCTGAGTTTATCGGACAGC'), 1.997696811857739),\n",
       " (Seq('ACCACACGGATATCCGGGTGTTAAGCCTGTA'), 1.997696811857739),\n",
       " (Seq('CCACACGGATATCCGGGTGTTAAGCCTGTAT'), 1.997696811857739),\n",
       " (Seq('ATCGGGCCTGGTTATCCCAATGATACGCTAA'), 1.997696811857739),\n",
       " (Seq('TCGGGCCTGGTTATCCCAATGATACGCTAAA'), 1.997696811857739),\n",
       " (Seq('CGGGCCTGGTTATCCCAATGATACGCTAAAG'), 1.997696811857739),\n",
       " (Seq('GGGCCTGGTTATCCCAATGATACGCTAAAGC'), 1.997696811857739),\n",
       " (Seq('ATGATGGCGAGCATCGTCTCTTATCAGGACA'), 1.997696811857739),\n",
       " (Seq('GATGGCGAGCATCGTCTCTTATCAGGACATC'), 1.997696811857739),\n",
       " (Seq('TTCAGGCGCGTCATTTACGGGCAAGACCATT'), 1.997696811857739),\n",
       " (Seq('TCATTTACGGGCAAGACCATTGAGCGTGTCA'), 1.997696811857739),\n",
       " (Seq('TTCATGGCCCTGAAAACCCTGATGAGACGTG'), 1.997696811857739),\n",
       " (Seq('AACCCTGATGAGACGTGGCATTACGATTTCC'), 1.997696811857739),\n",
       " (Seq('ACCCTGATGAGACGTGGCATTACGATTTCCA'), 1.997696811857739),\n",
       " (Seq('CTGATGAGACGTGGCATTACGATTTCCACCA'), 1.997696811857739),\n",
       " (Seq('TGATGAGACGTGGCATTACGATTTCCACCAC'), 1.997696811857739),\n",
       " (Seq('GCGTGACTAACCTCGATGCGATGGATATTAC'), 1.997696811857739),\n",
       " (Seq('GACTAACCTCGATGCGATGGATATTACTGCG'), 1.997696811857739),\n",
       " (Seq('ACTAACCTCGATGCGATGGATATTACTGCGC'), 1.997696811857739),\n",
       " (Seq('TAACCTCGATGCGATGGATATTACTGCGCCG'), 1.997696811857739),\n",
       " (Seq('AACCTCGATGCGATGGATATTACTGCGCCGT'), 1.997696811857739),\n",
       " (Seq('ACCTCGATGCGATGGATATTACTGCGCCGTA'), 1.997696811857739),\n",
       " (Seq('TAATGACCACGTTCTGCATGAGCGAGATCCG'), 1.997696811857739),\n",
       " (Seq('ATCATGCGACCGGCGTACAGTCAGCGTTTAA'), 1.997696811857739),\n",
       " (Seq('CGCGATCTTACCGAACTGTCTTTGCGGAAGA'), 1.997696811857739),\n",
       " (Seq('TGGCACTGTGGCGGTAATCGCACTTTAACAA'), 1.997696811857739),\n",
       " (Seq('GGCACTGTGGCGGTAATCGCACTTTAACAAT'), 1.997696811857739),\n",
       " (Seq('GCACTGTGGCGGTAATCGCACTTTAACAATC'), 1.997696811857739),\n",
       " (Seq('GTGGCGGTAATCGCACTTTAACAATCAGTGC'), 1.997696811857739),\n",
       " (Seq('GGCGGTAATCGCACTTTAACAATCAGTGCTG'), 1.997696811857739),\n",
       " (Seq('CTGGAGATTACCGCCCAGGATAATTTACTGG'), 1.997696811857739),\n",
       " (Seq('AGAACGTACTTACCTGTATCAGGGCATTGCC'), 1.997696811857739),\n",
       " (Seq('GAACGTACTTACCTGTATCAGGGCATTGCCG'), 1.997696811857739),\n",
       " (Seq('AACGTACTTACCTGTATCAGGGCATTGCCGA'), 1.997696811857739),\n",
       " (Seq('ACGTACTTACCTGTATCAGGGCATTGCCGAG'), 1.997696811857739),\n",
       " (Seq('ACTTACCTGTATCAGGGCATTGCCGAGCGTA'), 1.997696811857739),\n",
       " (Seq('CTTACCTGTATCAGGGCATTGCCGAGCGTAA'), 1.997696811857739),\n",
       " (Seq('TTACCTGTATCAGGGCATTGCCGAGCGTAAC'), 1.997696811857739),\n",
       " (Seq('TACCTGTATCAGGGCATTGCCGAGCGTAACT'), 1.997696811857739),\n",
       " (Seq('ACCTGTATCAGGGCATTGCCGAGCGTAACTT'), 1.997696811857739),\n",
       " (Seq('GTTCCAGTTAGCGGAAAACATTCACGTTCGC'), 1.997696811857739),\n",
       " (Seq('TTCCAGTTAGCGGAAAACATTCACGTTCGCG'), 1.997696811857739),\n",
       " (Seq('TCCAGTTAGCGGAAAACATTCACGTTCGCGG'), 1.997696811857739),\n",
       " (Seq('CAAACCTGGTCAACGGGCTGCTGTATATCGA'), 1.997696811857739),\n",
       " (Seq('AACCTGGTCAACGGGCTGCTGTATATCGAAC'), 1.997696811857739),\n",
       " (Seq('ACCTGGTCAACGGGCTGCTGTATATCGAACT'), 1.997696811857739),\n",
       " (Seq('GCGCCGTATCGAAATTAACTGATTCCGTCGG'), 1.997696811857739),\n",
       " (Seq('CGCCGTATCGAAATTAACTGATTCCGTCGGG'), 1.997696811857739),\n",
       " (Seq('ATCTGGATATTCAACTGGAAGGCACGCGCCT'), 1.997696811857739),\n",
       " (Seq('AGCCCAAATGGTTACATCAGGGCCTGGTCAT'), 1.997696811857739),\n",
       " (Seq('CCAAATGGTTACATCAGGGCCTGGTCATGCA'), 1.997696811857739),\n",
       " (Seq('AAATATGGAAGTTTCCGGCGCGACGTTTACC'), 1.997696811857739),\n",
       " (Seq('AATATGGAAGTTTCCGGCGCGACGTTTACCA'), 1.997696811857739),\n",
       " (Seq('ATATGGAAGTTTCCGGCGCGACGTTTACCAA'), 1.997696811857739),\n",
       " (Seq('TATGGAAGTTTCCGGCGCGACGTTTACCAAC'), 1.997696811857739),\n",
       " (Seq('GCTGCATATTGATTTAACCCGTAACGAGCCG'), 1.997696811857739),\n",
       " (Seq('CTGCATATTGATTTAACCCGTAACGAGCCGG'), 1.997696811857739),\n",
       " (Seq('AGCGGCGATATGCTGCACTTTATCCAGGAAT'), 1.997696811857739),\n",
       " (Seq('GCGGGTTAGTCACCGCGATTCTGCATAAAAT'), 1.997696811857739),\n",
       " (Seq('ATGGCTATTCAGGATGTGCCGATCCTGAACA'), 1.997696811857739),\n",
       " (Seq('CTATTCAGGATGTGCCGATCCTGAACAGCGA'), 1.997696811857739),\n",
       " (Seq('TATTCAGGATGTGCCGATCCTGAACAGCGAT'), 1.997696811857739),\n",
       " (Seq('AAAGTGCTCGGCAAACGTATCCGCGATTTGC'), 1.997696811857739),\n",
       " (Seq('AAGTGCTCGGCAAACGTATCCGCGATTTGCA'), 1.997696811857739),\n",
       " (Seq('CTGAAATCCGGCGGCGATTTTGTCGATACAC'), 1.997696811857739),\n",
       " (Seq('AAATCCGGCGGCGATTTTGTCGATACACTGA'), 1.997696811857739),\n",
       " (Seq('AATCCGGCGGCGATTTTGTCGATACACTGAC'), 1.997696811857739),\n",
       " (Seq('TTGCCAAAATGAACTATCTGACGCTGTGCGG'), 1.997696811857739),\n",
       " (Seq('GTATTATCACGCCACAACTGCTGGCGGTGAT'), 1.997696811857739),\n",
       " (Seq('AGCCTACGCACTGTGTTAAATCCAGGCTGGA'), 1.997696811857739),\n",
       " (Seq('GCCTACGCACTGTGTTAAATCCAGGCTGGAT'), 1.997696811857739),\n",
       " (Seq('CCTACGCACTGTGTTAAATCCAGGCTGGATA'), 1.997696811857739),\n",
       " (Seq('CGCACTGTGTTAAATCCAGGCTGGATAACGC'), 1.997696811857739),\n",
       " (Seq('CACTGTGTTAAATCCAGGCTGGATAACGCGC'), 1.997696811857739),\n",
       " (Seq('CCCTTGCTGCGGGATAATGTGAAGCATATCA'), 1.997696811857739),\n",
       " (Seq('AAAGGAGTCGTCATGAAGATTTCCCGCCTCG'), 1.997696811857739),\n",
       " (Seq('AGTCGTCATGAAGATTTCCCGCCTCGGAGAA'), 1.997696811857739),\n",
       " (Seq('GCCGGATTATCGCTTCTCACTGGCAAATGAG'), 1.997696811857739),\n",
       " (Seq('CGGATTATCGCTTCTCACTGGCAAATGAGCG'), 1.997696811857739),\n",
       " (Seq('GGATTATCGCTTCTCACTGGCAAATGAGCGC'), 1.997696811857739),\n",
       " (Seq('GATTATCGCTTCTCACTGGCAAATGAGCGCA'), 1.997696811857739),\n",
       " (Seq('CGATGCGTCTGAAAGAGGATTTGCCCTATAC'), 1.997696811857739),\n",
       " (Seq('GATGCGTCTGAAAGAGGATTTGCCCTATACC'), 1.997696811857739),\n",
       " (Seq('ATGCGTCTGAAAGAGGATTTGCCCTATACCC'), 1.997696811857739),\n",
       " (Seq('TGCGTCTGAAAGAGGATTTGCCCTATACCCA'), 1.997696811857739),\n",
       " (Seq('GCGTCTGAAAGAGGATTTGCCCTATACCCAT'), 1.997696811857739),\n",
       " (Seq('TACTGTATGCCGGATAGTCGTAAAGCCCGTC'), 1.997696811857739),\n",
       " (Seq('TGATGACCAGATTCAGGTCGTCGTAAATCGC'), 1.997696811857739),\n",
       " (Seq('GATGACCAGATTCAGGTCGTCGTAAATCGCC'), 1.997696811857739),\n",
       " (Seq('TGACCAGATTCAGGTCGTCGTAAATCGCCAG'), 1.997696811857739),\n",
       " (Seq('GTAACTTACAACGGCTCGGCATTCGCAATGT'), 1.997696811857739),\n",
       " (Seq('TTATCCCATTGTTGGAACATGACCGGAACGG'), 1.997696811857739),\n",
       " (Seq('ACGTGAAGATATTCAGCGTATCTCGGTCCGC'), 1.997696811857739),\n",
       " (Seq('CCGCTAAGCGCTGAATGTCAGAAGTGCTCTT'), 1.997696811857739),\n",
       " (Seq('AAATCAGCTTTGCGAGGGCTACCAGACCTTT'), 1.997696811857739),\n",
       " (Seq('CGATGCTGCGTTAACGTCACAGGCCTGATAA'), 1.997696811857739),\n",
       " (Seq('ATGCTGCGTTAACGTCACAGGCCTGATAAGC'), 1.997696811857739),\n",
       " (Seq('CTGCGTTAACGTCACAGGCCTGATAAGCGTA'), 1.997696811857739),\n",
       " (Seq('AACAGTGGACGTTGTCGCCAAACGCCAGTTT'), 1.997696811857739),\n",
       " (Seq('CCCAGAAATAGCGTGCGAGAGTTTTCATCGT'), 1.997696811857739),\n",
       " (Seq('CCAGAAATAGCGTGCGAGAGTTTTCATCGTC'), 1.997696811857739),\n",
       " (Seq('GGCGTCGGCAGACATATGTACCGTCACTTTA'), 1.997696811857739),\n",
       " (Seq('GCGTCGGCAGACATATGTACCGTCACTTTAA'), 1.997696811857739),\n",
       " (Seq('AAGCAACACGCTATAGTCATCGTCGGTGGTT'), 1.997696811857739),\n",
       " (Seq('AGCAACACGCTATAGTCATCGTCGGTGGTTA'), 1.997696811857739),\n",
       " (Seq('GCAACACGCTATAGTCATCGTCGGTGGTTAA'), 1.997696811857739),\n",
       " (Seq('CAACACGCTATAGTCATCGTCGGTGGTTAAC'), 1.997696811857739),\n",
       " (Seq('ACACGCTATAGTCATCGTCGGTGGTTAACAG'), 1.997696811857739),\n",
       " (Seq('CACGCTATAGTCATCGTCGGTGGTTAACAGA'), 1.997696811857739),\n",
       " (Seq('ACGCTATAGTCATCGTCGGTGGTTAACAGAC'), 1.997696811857739),\n",
       " (Seq('CGCTATAGTCATCGTCGGTGGTTAACAGACC'), 1.997696811857739),\n",
       " (Seq('GCTATAGTCATCGTCGGTGGTTAACAGACCC'), 1.997696811857739),\n",
       " (Seq('CTATAGTCATCGTCGGTGGTTAACAGACCCG'), 1.997696811857739),\n",
       " (Seq('TATAGTCATCGTCGGTGGTTAACAGACCCGC'), 1.997696811857739),\n",
       " (Seq('CGCCAGCAGAATCGGGTGCATATGCATATTT'), 1.997696811857739),\n",
       " (Seq('GTGCATATGCATATTTGAGAGGATCACCGCC'), 1.997696811857739),\n",
       " (Seq('TATGCATATTTGAGAGGATCACCGCCAGCGT'), 1.997696811857739),\n",
       " (Seq('CCAGCCCGCTACTTTTGAGAATGGCGTTGAA'), 1.997696811857739),\n",
       " (Seq('CAGCCCGCTACTTTTGAGAATGGCGTTGAAC'), 1.997696811857739),\n",
       " (Seq('TGGGTAAGTAGCGCGCTCATACCCATATGCT'), 1.997696811857739),\n",
       " (Seq('ATACCCATATGCTGGCGCAGACCGAGTATGT'), 1.997696811857739),\n",
       " (Seq('TACCCATATGCTGGCGCAGACCGAGTATGTA'), 1.997696811857739),\n",
       " (Seq('CCCATATGCTGGCGCAGACCGAGTATGTAAT'), 1.997696811857739),\n",
       " (Seq('CATTGCGGTGATCGGATTACCGATAAACTCC'), 1.997696811857739),\n",
       " (Seq('TTGCGGTGATCGGATTACCGATAAACTCCAG'), 1.997696811857739),\n",
       " (Seq('TGCGGTGATCGGATTACCGATAAACTCCAGC'), 1.997696811857739),\n",
       " (Seq('TTTATTTGCGACAAACAGCGCAGCCGGATGC'), 1.997696811857739),\n",
       " (Seq('CGGAAACCTCCATCATTTTGCCGAGGATGGT'), 1.997696811857739),\n",
       " (Seq('GAAACCTCCATCATTTTGCCGAGGATGGTGC'), 1.997696811857739),\n",
       " (Seq('CTCCATCATTTTGCCGAGGATGGTGCCAAGA'), 1.997696811857739),\n",
       " (Seq('TCCATCATTTTGCCGAGGATGGTGCCAAGAC'), 1.997696811857739),\n",
       " (Seq('CGATTAACACGATGCTGATCAGCAGCGTGCT'), 1.997696811857739),\n",
       " (Seq('GATTAACACGATGCTGATCAGCAGCGTGCTC'), 1.997696811857739),\n",
       " (Seq('AAGATGACTGAGGTTGCCATGGAACCCCTTC'), 1.997696811857739),\n",
       " (Seq('TATACTTTTGAAGTCGCGGCCAGACACCAGT'), 1.997696811857739),\n",
       " (Seq('TCGTTAAGCCCCAGTGCGGTAAGTCATCGTA'), 1.997696811857739),\n",
       " (Seq('CGTTAAGCCCCAGTGCGGTAAGTCATCGTAT'), 1.997696811857739),\n",
       " (Seq('GTTAAGCCCCAGTGCGGTAAGTCATCGTATC'), 1.997696811857739),\n",
       " (Seq('TTAAGCCCCAGTGCGGTAAGTCATCGTATCA'), 1.997696811857739),\n",
       " (Seq('CGCTGAAATCGTCGCTGGATACGTTGAATCA'), 1.997696811857739),\n",
       " (Seq('TCGTCGCTGGATACGTTGAATCAGGAAATCC'), 1.997696811857739),\n",
       " (Seq('CGTCGCTGGATACGTTGAATCAGGAAATCCT'), 1.997696811857739),\n",
       " (Seq('GTCGCTGGATACGTTGAATCAGGAAATCCTC'), 1.997696811857739),\n",
       " (Seq('TCGCTGGATACGTTGAATCAGGAAATCCTCG'), 1.997696811857739),\n",
       " (Seq('GATAACGTTAACCTACAGCGTGCCGGCATTG'), 1.997696811857739),\n",
       " (Seq('ATTTAGCGGTGATTGCCGCGATGAACCACAT'), 1.997696811857739),\n",
       " (Seq('CAGTGGCCGAAAATCGAGGCATTTATTCGCT'), 1.997696811857739),\n",
       " (Seq('TCGCTACACCTGCCGGGAGTGAATATGCTAA'), 1.997696811857739),\n",
       " (Seq('GCGCCTCATCCATTTGCGTAAGCTGGTAGAA'), 1.997696811857739),\n",
       " (Seq('CGCCTCATCCATTTGCGTAAGCTGGTAGAAG'), 1.997696811857739),\n",
       " (Seq('GCCTCATCCATTTGCGTAAGCTGGTAGAAGC'), 1.997696811857739),\n",
       " (Seq('CATCCATTTGCGTAAGCTGGTAGAAGCCTGC'), 1.997696811857739),\n",
       " (Seq('CTCAATCATAATATGGCGTCGCTGAATCGCG'), 1.997696811857739),\n",
       " (Seq('AACTATGTACTGGCGGAGTGGCACATCTTCC'), 1.997696811857739),\n",
       " (Seq('TGCCCGTTTTATGGCCTGCGAGCTGAAAAAA'), 1.997696811857739),\n",
       " (Seq('CCCGTTTTATGGCCTGCGAGCTGAAAAAAGC'), 1.997696811857739),\n",
       " (Seq('TCCCGTATGCGATAAACGCGGATACTCAGTT'), 1.997696811857739),\n",
       " (Seq('CCCGTATGCGATAAACGCGGATACTCAGTTT'), 1.997696811857739),\n",
       " (Seq('GGAACGTTAAACCGTCTGTCCCTGATGCGAT'), 1.997696811857739),\n",
       " (Seq('GAACGTTAAACCGTCTGTCCCTGATGCGATA'), 1.997696811857739),\n",
       " (Seq('GCGCCGTTACCAAACAGCGTTTTATAGCTGG'), 1.997696811857739),\n",
       " (Seq('GCCGTTACCAAACAGCGTTTTATAGCTGGCG'), 1.997696811857739),\n",
       " (Seq('CCGTTACCAAACAGCGTTTTATAGCTGGCGA'), 1.997696811857739),\n",
       " (Seq('CAAACAGCGTTTTATAGCTGGCGATCAGCCG'), 1.997696811857739),\n",
       " (Seq('AAACAGCGTTTTATAGCTGGCGATCAGCCGT'), 1.997696811857739),\n",
       " (Seq('TGGCATGACGAAGCTGCGTTCCTTCATACAG'), 1.997696811857739),\n",
       " (Seq('GGCATGACGAAGCTGCGTTCCTTCATACAGA'), 1.997696811857739),\n",
       " (Seq('GCATGACGAAGCTGCGTTCCTTCATACAGAT'), 1.997696811857739),\n",
       " (Seq('ATGACGAAGCTGCGTTCCTTCATACAGATCG'), 1.997696811857739),\n",
       " (Seq('ACACCGTCAAACTATGCGTGGTCATGGCGAT'), 1.997696811857739),\n",
       " (Seq('ACCGTCAAACTATGCGTGGTCATGGCGATCA'), 1.997696811857739),\n",
       " (Seq('CGGGAATATAAATGGTTTGCGCCATCTGCCC'), 1.997696811857739),\n",
       " (Seq('ACTTGCTGCGCATTCCCATAAATGCGAGAGG'), 1.997696811857739),\n",
       " (Seq('CTTGCTGCGCATTCCCATAAATGCGAGAGGA'), 1.997696811857739),\n",
       " (Seq('ACCTGATAGGTTTTCGCCACCAGGACGGTAT'), 1.997696811857739),\n",
       " (Seq('CCTGATAGGTTTTCGCCACCAGGACGGTATA'), 1.997696811857739),\n",
       " (Seq('CTGATAGGTTTTCGCCACCAGGACGGTATAC'), 1.997696811857739),\n",
       " (Seq('TGATAGGTTTTCGCCACCAGGACGGTATACA'), 1.997696811857739),\n",
       " (Seq('GATAGGTTTTCGCCACCAGGACGGTATACAC'), 1.997696811857739),\n",
       " (Seq('GAATGGTGATATCCACTACGCTCATACGCGT'), 1.997696811857739),\n",
       " (Seq('TGCGTGTTGACATAACACAGTGTGCTCACGA'), 1.997696811857739),\n",
       " (Seq('ATCAAAATTCGCGCCGAAATTGCGCGTTCTG'), 1.997696811857739),\n",
       " (Seq('CGAAATTGCGCGTTCTGCACAGAACGCGTAT'), 1.997696811857739),\n",
       " (Seq('AATTGCGCGTTCTGCACAGAACGCGTATACT'), 1.997696811857739),\n",
       " (Seq('GCACAGAACGCGTATACTTCTGCTGTTGACA'), 1.997696811857739),\n",
       " (Seq('GTTGACATAACACAGTGTGCTTCGCGGCTAC'), 1.997696811857739),\n",
       " (Seq('TACTGACGCGTTTCAGGAAGTGGATACCTAC'), 1.997696811857739),\n",
       " (Seq('CAGGAAGTGGATACCTACGGTATCTCTATCC'), 1.997696811857739),\n",
       " (Seq('AGGAAGTGGATACCTACGGTATCTCTATCCC'), 1.997696811857739),\n",
       " (Seq('GATGCACGGCGCGCGCAGTACCAATTTTATT'), 1.997696811857739),\n",
       " (Seq('GGCGCGCGCAGTACCAATTTTATTCTGCAAG'), 1.997696811857739),\n",
       " (Seq('GCGCGCGCAGTACCAATTTTATTCTGCAAGA'), 1.997696811857739),\n",
       " (Seq('CGCGCGCAGTACCAATTTTATTCTGCAAGAG'), 1.997696811857739),\n",
       " (Seq('GCGCGCAGTACCAATTTTATTCTGCAAGAGG'), 1.997696811857739),\n",
       " (Seq('CGCGCAGTACCAATTTTATTCTGCAAGAGGC'), 1.997696811857739),\n",
       " (Seq('GCGCAGTACCAATTTTATTCTGCAAGAGGCC'), 1.997696811857739),\n",
       " (Seq('CGCAGTACCAATTTTATTCTGCAAGAGGCCG'), 1.997696811857739),\n",
       " (Seq('AATCATCCATGTGGATATCGATCGCGCCGAG'), 1.997696811857739),\n",
       " (Seq('ATCATCCATGTGGATATCGATCGCGCCGAGT'), 1.997696811857739),\n",
       " (Seq('CATCCATGTGGATATCGATCGCGCCGAGTTA'), 1.997696811857739),\n",
       " (Seq('GTACTGGCACAGTTAATTCCCCATATCGAGG'), 1.997696811857739),\n",
       " (Seq('GCGGACTTGCAGCAAGAATTCCCTTGCAGTA'), 1.997696811857739),\n",
       " (Seq('CGGACTTGCAGCAAGAATTCCCTTGCAGTAT'), 1.997696811857739),\n",
       " (Seq('GCCTGTGTGGACGACAACGCGATTATTACCA'), 1.997696811857739),\n",
       " (Seq('CTGTGTGGACGACAACGCGATTATTACCACT'), 1.997696811857739),\n",
       " (Seq('TGTGTGGACGACAACGCGATTATTACCACTG'), 1.997696811857739),\n",
       " (Seq('CGCGATTATTACCACTGACGTAGGTCAGCAT'), 1.997696811857739),\n",
       " (Seq('GCGATTATTACCACTGACGTAGGTCAGCATC'), 1.997696811857739),\n",
       " (Seq('TTACCACTGACGTAGGTCAGCATCAGATGTG'), 1.997696811857739),\n",
       " (Seq('TTATCCTGATGAATAACGACGCGCTGGGACT'), 1.997696811857739),\n",
       " (Seq('GGACTGGTACATCAGCAACAGAGTCTGTTCT'), 1.997696811857739),\n",
       " (Seq('ATAAACAGGGCGTTTTTGCGGCAACCTATCC'), 1.997696811857739),\n",
       " (Seq('TAAACAGGGCGTTTTTGCGGCAACCTATCCG'), 1.997696811857739),\n",
       " (Seq('TTTTTGCGGCAACCTATCCGGGGAGCATCAA'), 1.997696811857739),\n",
       " (Seq('GGCAACCTATCCGGGGAGCATCAACTTTATG'), 1.997696811857739),\n",
       " (Seq('CCTATCCGGGGAGCATCAACTTTATGCAGAT'), 1.997696811857739),\n",
       " (Seq('ATCCGGGGAGCATCAACTTTATGCAGATTGC'), 1.997696811857739),\n",
       " (Seq('TCCGGGGAGCATCAACTTTATGCAGATTGCC'), 1.997696811857739),\n",
       " (Seq('AGCATCAACTTTATGCAGATTGCCGCCGGGT'), 1.997696811857739),\n",
       " (Seq('CTCTGTTTACCGATTCAGGGCAGCGATCAGA'), 1.997696811857739),\n",
       " (Seq('ATCAGAGCCGCATCTGGCTTCTGGTCAATGA'), 1.997696811857739),\n",
       " (Seq('CTTCTGGTCAATGACGATCAGCGGCTTGAAC'), 1.997696811857739),\n",
       " (Seq('TTCTGGTCAATGACGATCAGCGGCTTGAACA'), 1.997696811857739),\n",
       " (Seq('ATCAGGCGTGATGACTTTGACGGAACCATTC'), 1.997696811857739),\n",
       " (Seq('TCAGGCGTGATGACTTTGACGGAACCATTCC'), 1.997696811857739),\n",
       " (Seq('GCGTGATGACTTTGACGGAACCATTCCGACA'), 1.997696811857739),\n",
       " (Seq('CGTGATGACTTTGACGGAACCATTCCGACAG'), 1.997696811857739),\n",
       " (Seq('GTGATGACTTTGACGGAACCATTCCGACAGT'), 1.997696811857739),\n",
       " (Seq('TGATGACTTTGACGGAACCATTCCGACAGTG'), 1.997696811857739),\n",
       " (Seq('TGACTTTGACGGAACCATTCCGACAGTGAGC'), 1.997696811857739),\n",
       " (Seq('TGTACGCCGCTGGCGGAAATAAACACTTTGC'), 1.997696811857739),\n",
       " (Seq('ATTGGCATCATAAAAGGCATCGCTCTTGGCC'), 1.997696811857739),\n",
       " (Seq('TCATAAAAGGCATCGCTCTTGGCCCGATAGG'), 1.997696811857739),\n",
       " (Seq('CATAAAAGGCATCGCTCTTGGCCCGATAGGT'), 1.997696811857739),\n",
       " (Seq('GCACAAAATCGCCGTGGCATTAGGCTTTTCA'), 1.997696811857739),\n",
       " (Seq('CATGCTGATAACTAATGGCATTTCCGGACCG'), 1.997696811857739),\n",
       " (Seq('AGGCTTATTTACCATGACGATATAGCCGCCG'), 1.997696811857739),\n",
       " (Seq('GGCTTATTTACCATGACGATATAGCCGCCGA'), 1.997696811857739),\n",
       " (Seq('GCTTATTTACCATGACGATATAGCCGCCGAG'), 1.997696811857739),\n",
       " (Seq('TGGCACTACTATACGTCTGGCTGTCAGGAAA'), 1.997696811857739),\n",
       " (Seq('GGCACTACTATACGTCTGGCTGTCAGGAAAT'), 1.997696811857739),\n",
       " (Seq('AGTGGGCGACGATATTTTTGCCGACAACACC'), 1.997696811857739),\n",
       " (Seq('GTGGGCGACGATATTTTTGCCGACAACACCA'), 1.997696811857739),\n",
       " (Seq('TGGGCGACGATATTTTTGCCGACAACACCAT'), 1.997696811857739),\n",
       " (Seq('GGGCGACGATATTTTTGCCGACAACACCATT'), 1.997696811857739),\n",
       " (Seq('ACACCATTCGTAATCTCGAATCCTGGGGGAT'), 1.997696811857739),\n",
       " (Seq('ACCATTCGTAATCTCGAATCCTGGGGGATCA'), 1.997696811857739),\n",
       " (Seq('CCATTCGTAATCTCGAATCCTGGGGGATCAA'), 1.997696811857739),\n",
       " (Seq('TCGTAATCTCGAATCCTGGGGGATCAATACG'), 1.997696811857739),\n",
       " (Seq('TCTCGAATCCTGGGGGATCAATACGACGTAT'), 1.997696811857739),\n",
       " (Seq('ACAAGTTTCTCTCGCCGGAAGATATCGATCG'), 1.997696811857739),\n",
       " (Seq('AAGTTTCTCTCGCCGGAAGATATCGATCGCG'), 1.997696811857739),\n",
       " (Seq('GGATTGAAGTGTTATTAAACCCTGCGCCAGC'), 1.997696811857739),\n",
       " (Seq('AGTGTTATTAAACCCTGCGCCAGCATTACGG'), 1.997696811857739),\n",
       " (Seq('GTGTTATTAAACCCTGCGCCAGCATTACGGG'), 1.997696811857739),\n",
       " (Seq('TGTTATTAAACCCTGCGCCAGCATTACGGGA'), 1.997696811857739),\n",
       " (Seq('CCCTGCGCCAGCATTACGGGAATTAGATATG'), 1.997696811857739),\n",
       " (Seq('CCTGCGCCAGCATTACGGGAATTAGATATGT'), 1.997696811857739),\n",
       " (Seq('CTGCGCCAGCATTACGGGAATTAGATATGTC'), 1.997696811857739),\n",
       " (Seq('TTAACCGGTATGCCAGTGGATACCTATGACC'), 1.997696811857739),\n",
       " (Seq('CCGGTATGCCAGTGGATACCTATGACCATAT'), 1.997696811857739),\n",
       " (Seq('ATGCCAGTGGATACCTATGACCATATTCGCG'), 1.997696811857739),\n",
       " (Seq('AGTGGATACCTATGACCATATTCGCGCAGCG'), 1.997696811857739),\n",
       " (Seq('AAACATCGTTCAGATGCCCGACGGGTATCTG'), 1.997696811857739),\n",
       " (Seq('AACATCGTTCAGATGCCCGACGGGTATCTGA'), 1.997696811857739),\n",
       " (Seq('ACATCGTTCAGATGCCCGACGGGTATCTGAA'), 1.997696811857739),\n",
       " (Seq('CATCGTTCAGATGCCCGACGGGTATCTGAAT'), 1.997696811857739),\n",
       " (Seq('ATCGTTCAGATGCCCGACGGGTATCTGAATA'), 1.997696811857739),\n",
       " (Seq('TCGTTCAGATGCCCGACGGGTATCTGAATAA'), 1.997696811857739),\n",
       " (Seq('CCGACGGGTATCTGAATAAGACACCTCTGTT'), 1.997696811857739),\n",
       " (Seq('CGACGGGTATCTGAATAAGACACCTCTGTTC'), 1.997696811857739),\n",
       " (Seq('GACGGGTATCTGAATAAGACACCTCTGTTCC'), 1.997696811857739),\n",
       " (Seq('CGGGTATCTGAATAAGACACCTCTGTTCCAG'), 1.997696811857739),\n",
       " (Seq('GCAACTTTGCTTCGGCATTAGTACAAAGCGC'), 1.997696811857739),\n",
       " (Seq('TATAAAGTGGCGATACTTATCGGCCTGACGC'), 1.997696811857739),\n",
       " (Seq('ATAAAGTGGCGATACTTATCGGCCTGACGCT'), 1.997696811857739),\n",
       " (Seq('TAAAGTGGCGATACTTATCGGCCTGACGCTA'), 1.997696811857739),\n",
       " (Seq('AAAGTGGCGATACTTATCGGCCTGACGCTAT'), 1.997696811857739),\n",
       " (Seq('AAGTGGCGATACTTATCGGCCTGACGCTATA'), 1.997696811857739),\n",
       " (Seq('AGTGGCGATACTTATCGGCCTGACGCTATAC'), 1.997696811857739),\n",
       " (Seq('GTGGCGATACTTATCGGCCTGACGCTATACA'), 1.997696811857739),\n",
       " (Seq('TTTCCTGGAAACGGCGGCGAATACCTACAGT'), 1.997696811857739),\n",
       " (Seq('TTCCTGGAAACGGCGGCGAATACCTACAGTT'), 1.997696811857739),\n",
       " (Seq('GCAAGTCCACAACTTCAAGGTGCTGATGCTG'), 1.997696811857739),\n",
       " (Seq('CAAGTCCACAACTTCAAGGTGCTGATGCTGG'), 1.997696811857739),\n",
       " (Seq('CTTCAAGGTGCTGATGCTGGAAAACACTCTG'), 1.997696811857739),\n",
       " (Seq('GTGCTGATGCTGGAAAACACTCTGGAACCCT'), 1.997696811857739),\n",
       " (Seq('ATCAATGAGCGCGATGCCTCAACCTTTATGG'), 1.997696811857739),\n",
       " (Seq('ATTTATGCCGGAACGCTCGATACCGTCGATA'), 1.997696811857739),\n",
       " (Seq('TTTATGCCGGAACGCTCGATACCGTCGATAA'), 1.997696811857739),\n",
       " (Seq('TACAAAACAGCCGCGGTCATCTGGTGATTCT'), 1.997696811857739),\n",
       " (Seq('ACAAAACAGCCGCGGTCATCTGGTGATTCTG'), 1.997696811857739),\n",
       " (Seq('TGACGGTCATGATCTGACTATGCGCAACATG'), 1.997696811857739),\n",
       " (Seq('GACGGTCATGATCTGACTATGCGCAACATGT'), 1.997696811857739),\n",
       " (Seq('TCATCATTATCAGGCGCAACCTGCGGTGGTA'), 1.997696811857739),\n",
       " (Seq('CATCATTATCAGGCGCAACCTGCGGTGGTAA'), 1.997696811857739),\n",
       " (Seq('ATCATTATCAGGCGCAACCTGCGGTGGTAAT'), 1.997696811857739),\n",
       " (Seq('TCAGATGACAGTGACTAACCTGGCTTCAGTC'), 1.997696811857739),\n",
       " (Seq('CAGATGACAGTGACTAACCTGGCTTCAGTCG'), 1.997696811857739),\n",
       " (Seq('AGATGACAGTGACTAACCTGGCTTCAGTCGC'), 1.997696811857739),\n",
       " (Seq('ATGTGCCACATGAACTATGCCTATGTGCCGA'), 1.997696811857739),\n",
       " (Seq('TGTGCCACATGAACTATGCCTATGTGCCGAA'), 1.997696811857739),\n",
       " (Seq('TAATCAACGACTTTTGCAGGGTGAAGCCTCG'), 1.997696811857739),\n",
       " (Seq('AATCAACGACTTTTGCAGGGTGAAGCCTCGC'), 1.997696811857739),\n",
       " (Seq('ATCAACGACTTTTGCAGGGTGAAGCCTCGCT'), 1.997696811857739),\n",
       " (Seq('GAAATCGTGTTCTTCGCCGATGAACTGGACA'), 1.997696811857739),\n",
       " (Seq('AAATCGTGTTCTTCGCCGATGAACTGGACAG'), 1.997696811857739),\n",
       " (Seq('TGTTCTTCGCCGATGAACTGGACAGGTACAC'), 1.997696811857739),\n",
       " (Seq('GAATTCAGTATGATCGCCCCGGATGGCACTA'), 1.997696811857739),\n",
       " (Seq('TGACACGTTTCGCCAGCGCGGAGCTAAATTA'), 1.997696811857739),\n",
       " (Seq('CGGAGCTAAATTACGTGACCCGCTGGATTCT'), 1.997696811857739),\n",
       " (Seq('ACGTGACCCGCTGGATTCTTTATAACGGCGA'), 1.997696811857739),\n",
       " (Seq('GACCCGCTGGATTCTTTATAACGGCGATCAG'), 1.997696811857739),\n",
       " (Seq('CCGCTGGATTCTTTATAACGGCGATCAGCAG'), 1.997696811857739),\n",
       " (Seq('GATTCTTTATAACGGCGATCAGCAGGTAGCC'), 1.997696811857739),\n",
       " (Seq('ATTCTTTATAACGGCGATCAGCAGGTAGCCG'), 1.997696811857739),\n",
       " (Seq('TTCTTTATAACGGCGATCAGCAGGTAGCCGC'), 1.997696811857739),\n",
       " (Seq('TTTATAACGGCGATCAGCAGGTAGCCGCCTT'), 1.997696811857739),\n",
       " (Seq('TTATAACGGCGATCAGCAGGTAGCCGCCTTT'), 1.997696811857739),\n",
       " (Seq('ACAGGCATTGTCTGACCGCTCAAGGCTTGAA'), 1.997696811857739),\n",
       " (Seq('AGGCATTGTCTGACCGCTCAAGGCTTGAACA'), 1.997696811857739),\n",
       " (Seq('GGCTTGAACACCCAGGCACTTATCGTTAAGG'), 1.997696811857739),\n",
       " (Seq('GCTTGAACACCCAGGCACTTATCGTTAAGGT'), 1.997696811857739),\n",
       " (Seq('CACCCAGGCACTTATCGTTAAGGTAAGTGCT'), 1.997696811857739),\n",
       " (Seq('GCATGCCTTAGCGTCAGGATGAACCCATGAT'), 1.997696811857739),\n",
       " (Seq('CATGCCTTAGCGTCAGGATGAACCCATGATT'), 1.997696811857739),\n",
       " (Seq('TGCCTTAGCGTCAGGATGAACCCATGATTAC'), 1.997696811857739),\n",
       " (Seq('CTTAGCGTCAGGATGAACCCATGATTACCGT'), 1.997696811857739),\n",
       " (Seq('TAGCGTCAGGATGAACCCATGATTACCGTTG'), 1.997696811857739),\n",
       " (Seq('AGCGTCAGGATGAACCCATGATTACCGTTGC'), 1.997696811857739),\n",
       " (Seq('GATATTTCCGGACTGGAGCTGCTAAGCCAAC'), 1.997696811857739),\n",
       " (Seq('ATATTTCCGGACTGGAGCTGCTAAGCCAACT'), 1.997696811857739),\n",
       " (Seq('TATTTCCGGACTGGAGCTGCTAAGCCAACTG'), 1.997696811857739),\n",
       " (Seq('ATGGCGACGATCATGCTCTCGGTTCACGATA'), 1.997696811857739),\n",
       " (Seq('CTCTCTAAACGCTGTAGTCCGGATGAACTGA'), 1.997696811857739),\n",
       " (Seq('CTCTAAACGCTGTAGTCCGGATGAACTGATC'), 1.997696811857739),\n",
       " (Seq('TCTAAACGCTGTAGTCCGGATGAACTGATCG'), 1.997696811857739),\n",
       " (Seq('CTAAACGCTGTAGTCCGGATGAACTGATCGC'), 1.997696811857739),\n",
       " (Seq('TAAACGCTGTAGTCCGGATGAACTGATCGCC'), 1.997696811857739),\n",
       " (Seq('TGCATGTCCATCGCGCGAATCTGCTGGAAAA'), 1.997696811857739),\n",
       " (Seq('TCAGCAATGACGTTGAACTGGCGCATCGCAT'), 1.997696811857739),\n",
       " (Seq('ATTGCCACGCTGATGAATGCTATCGCGCTGA'), 1.997696811857739),\n",
       " (Seq('ATTGTCAGTCACCTCGACTGGCGAATTGACG'), 1.997696811857739),\n",
       " (Seq('TTGTCAGTCACCTCGACTGGCGAATTGACGA'), 1.997696811857739),\n",
       " (Seq('TGTCAGTCACCTCGACTGGCGAATTGACGAA'), 1.997696811857739),\n",
       " (Seq('CGATCAACGACTGGGGCAACCTGTATATGTC'), 1.997696811857739),\n",
       " (Seq('GATCAACGACTGGGGCAACCTGTATATGTCC'), 1.997696811857739),\n",
       " (Seq('ATCAACGACTGGGGCAACCTGTATATGTCCG'), 1.997696811857739),\n",
       " (Seq('TCAACGACTGGGGCAACCTGTATATGTCCGA'), 1.997696811857739),\n",
       " (Seq('ATTTGGTCACGGCCAACACGGCAGTATCGAT'), 1.997696811857739),\n",
       " (Seq('TCACGGCCAACACGGCAGTATCGATGTTTGA'), 1.997696811857739),\n",
       " (Seq('TCTTCAACGGTAACCGCGGACCGATGAATTT'), 1.997696811857739),\n",
       " (Seq('CGGTAACCGCGGACCGATGAATTTAATCTTC'), 1.997696811857739),\n",
       " (Seq('GGTAACCGCGGACCGATGAATTTAATCTTCG'), 1.997696811857739),\n",
       " (Seq('GTAACCGCGGACCGATGAATTTAATCTTCGC'), 1.997696811857739),\n",
       " (Seq('AACCGCGGACCGATGAATTTAATCTTCGCTG'), 1.997696811857739),\n",
       " (Seq('ACCTCACCTTTTTGCGCTGAATGGGGCAAAA'), 1.997696811857739),\n",
       " (Seq('GGAGTAATCCATGCTGGCCTTCTTAAACCAG'), 1.997696811857739),\n",
       " (Seq('GAGTAATCCATGCTGGCCTTCTTAAACCAGG'), 1.997696811857739),\n",
       " (Seq('GCGCAGAACGACATGATTTCTACCTACGGGT'), 1.997696811857739),\n",
       " (Seq('CGCAGAACGACATGATTTCTACCTACGGGTT'), 1.997696811857739),\n",
       " (Seq('GCAGAACGACATGATTTCTACCTACGGGTTG'), 1.997696811857739),\n",
       " (Seq('GAACGACATGATTTCTACCTACGGGTTGAGC'), 1.997696811857739),\n",
       " (Seq('ATTTCTACCTACGGGTTGAGCATGACCGAGC'), 1.997696811857739),\n",
       " (Seq('GAAAACGCTGGTTTCCTACTACGCTGACGGT'), 1.997696811857739),\n",
       " (Seq('AAAACGCTGGTTTCCTACTACGCTGACGGTA'), 1.997696811857739),\n",
       " (Seq('AAACGCTGGTTTCCTACTACGCTGACGGTAA'), 1.997696811857739),\n",
       " (Seq('AACGCTGGTTTCCTACTACGCTGACGGTAAA'), 1.997696811857739),\n",
       " (Seq('ACGCTGGTTTCCTACTACGCTGACGGTAAAA'), 1.997696811857739),\n",
       " (Seq('CGCTGGTTTCCTACTACGCTGACGGTAAAAA'), 1.997696811857739),\n",
       " (Seq('ATTGGTATCGACCAGTGGTCAACCGTCTACG'), 1.997696811857739),\n",
       " (Seq('CGGCTGTCGGTTTCGTACCGAAAAAAGCCAT'), 1.997696811857739),\n",
       " (Seq('GGCTGTCGGTTTCGTACCGAAAAAAGCCATC'), 1.997696811857739),\n",
       " (Seq('GCTGTCGGTTTCGTACCGAAAAAAGCCATCG'), 1.997696811857739),\n",
       " (Seq('CTGTCGGTTTCGTACCGAAAAAAGCCATCGG'), 1.997696811857739),\n",
       " (Seq('TGTCGGTTTCGTACCGAAAAAAGCCATCGGC'), 1.997696811857739),\n",
       " (Seq('CTGCGGACGGTATCAAAGGAACCTTCGCTTA'), 1.997696811857739),\n",
       " (Seq('TGCGGACGGTATCAAAGGAACCTTCGCTTAT'), 1.997696811857739),\n",
       " (Seq('GCGGACGGTATCAAAGGAACCTTCGCTTATC'), 1.997696811857739),\n",
       " (Seq('CGGACGGTATCAAAGGAACCTTCGCTTATCT'), 1.997696811857739),\n",
       " (Seq('GATTCGCCGCCTGAGCGAACGTCTGAATATT'), 1.997696811857739),\n",
       " (Seq('ATTCGCCGCCTGAGCGAACGTCTGAATATTG'), 1.997696811857739),\n",
       " (Seq('TTCGCCGCCTGAGCGAACGTCTGAATATTGA'), 1.997696811857739),\n",
       " (Seq('TCGCCGCCTGAGCGAACGTCTGAATATTGAT'), 1.997696811857739),\n",
       " (Seq('CCGCCTGAGCGAACGTCTGAATATTGATGTA'), 1.997696811857739),\n",
       " (Seq('TGCATCGCCCTGATGAGTCGCATTTGCAAAA'), 1.997696811857739),\n",
       " (Seq('GCATCGCCCTGATGAGTCGCATTTGCAAAAT'), 1.997696811857739),\n",
       " (Seq('CATCGCCCTGATGAGTCGCATTTGCAAAATG'), 1.997696811857739),\n",
       " (Seq('ATCGCCCTGATGAGTCGCATTTGCAAAATGC'), 1.997696811857739),\n",
       " (Seq('CTGATGAGTCGCATTTGCAAAATGCCGCTCA'), 1.997696811857739),\n",
       " (Seq('TGAGTCGCATTTGCAAAATGCCGCTCAAGTG'), 1.997696811857739),\n",
       " (Seq('GAGTCGCATTTGCAAAATGCCGCTCAAGTGT'), 1.997696811857739),\n",
       " (Seq('GCAAAATGCCGCTCAAGTGTTACTGATCTGG'), 1.997696811857739),\n",
       " (Seq('CAAAATGCCGCTCAAGTGTTACTGATCTGGC'), 1.997696811857739),\n",
       " (Seq('GCATCGGTTGCTACAAAAAGCGCGTTTAGCC'), 1.997696811857739),\n",
       " (Seq('CATCGGTTGCTACAAAAAGCGCGTTTAGCCG'), 1.997696811857739),\n",
       " (Seq('ATCGGTTGCTACAAAAAGCGCGTTTAGCCGC'), 1.997696811857739),\n",
       " (Seq('TCGGTTGCTACAAAAAGCGCGTTTAGCCGCA'), 1.997696811857739),\n",
       " (Seq('GCTTTTTGCGCGAAATGGAACCCGATATGCA'), 1.997696811857739),\n",
       " (Seq('CTTTTTGCGCGAAATGGAACCCGATATGCAG'), 1.997696811857739),\n",
       " (Seq('TATGCAGGAGATCAACGCGTTTCAACTGCGC'), 1.997696811857739),\n",
       " (Seq('ATGCAGGAGATCAACGCGTTTCAACTGCGCT'), 1.997696811857739),\n",
       " (Seq('TGCAGGAGATCAACGCGTTTCAACTGCGCTA'), 1.997696811857739),\n",
       " (Seq('GCAGGAGATCAACGCGTTTCAACTGCGCTAT'), 1.997696811857739),\n",
       " (Seq('GCGCTATAACGCCTTTTTTCAGCCGGAAGAG'), 1.997696811857739),\n",
       " (Seq('CGCTATAACGCCTTTTTTCAGCCGGAAGAGG'), 1.997696811857739),\n",
       " (Seq('ATTAACGGCCAGATGGCGCGATTCATCGTTA'), 1.997696811857739),\n",
       " (Seq('GCCAGATGGCGCGATTCATCGTTAGCCAAAT'), 1.997696811857739),\n",
       " (Seq('TAAAGCGTAGCCGCCATCGTATTTGCTAAGG'), 1.997696811857739),\n",
       " (Seq('TAGCCGCCATCGTATTTGCTAAGGGTGAACC'), 1.997696811857739),\n",
       " (Seq('AGCCGCCATCGTATTTGCTAAGGGTGAACCA'), 1.997696811857739),\n",
       " (Seq('CGCCATCGTATTTGCTAAGGGTGAACCACAG'), 1.997696811857739),\n",
       " (Seq('CACATCCCACTGTATCAACAGGTTGTGGAGT'), 1.997696811857739),\n",
       " (Seq('ACATCCCACTGTATCAACAGGTTGTGGAGTG'), 1.997696811857739),\n",
       " (Seq('ATTATGTTCGCCACCTCCCTAAAGAGGTGAG'), 1.997696811857739),\n",
       " (Seq('GATTTTGCCGATCACGTCATTACGCGAGAAC'), 1.997696811857739),\n",
       " (Seq('TTTTGCCGATCACGTCATTACGCGAGAACAG'), 1.997696811857739),\n",
       " (Seq('GAACAGAATTTTCCAACGGGTCTACCGGTTG'), 1.997696811857739),\n",
       " (Seq('ACAGAATTTTCCAACGGGTCTACCGGTTGAG'), 1.997696811857739),\n",
       " (Seq('CAGAATTTTCCAACGGGTCTACCGGTTGAGC'), 1.997696811857739),\n",
       " (Seq('AGAATTTTCCAACGGGTCTACCGGTTGAGCC'), 1.997696811857739),\n",
       " (Seq('AGGTGTCGCTATTCCACATACTGATCACAGG'), 1.997696811857739),\n",
       " (Seq('CAGGTATGTTCGCCAAAACGCCATTAGCGTT'), 1.997696811857739),\n",
       " (Seq('AGGTATGTTCGCCAAAACGCCATTAGCGTTG'), 1.997696811857739),\n",
       " (Seq('GTATGTTCGCCAAAACGCCATTAGCGTTGGA'), 1.997696811857739),\n",
       " (Seq('CCATTAGCGTTGGAATACTGGCAGAACCCGT'), 1.997696811857739),\n",
       " (Seq('CATTAGCGTTGGAATACTGGCAGAACCCGTC'), 1.997696811857739),\n",
       " (Seq('GCGTTGGAATACTGGCAGAACCCGTCAATTT'), 1.997696811857739),\n",
       " (Seq('CGTTGGAATACTGGCAGAACCCGTCAATTTC'), 1.997696811857739),\n",
       " (Seq('GTTGGAATACTGGCAGAACCCGTCAATTTCG'), 1.997696811857739),\n",
       " (Seq('TGTTGCTACTTCCACGGCTGTGGCAGAAAAA'), 1.997696811857739),\n",
       " (Seq('TTGCTACTTCCACGGCTGTGGCAGAAAAAGT'), 1.997696811857739),\n",
       " (Seq('TTGCCAAACAATACCGATGGTGCAGCTTTGG'), 1.997696811857739),\n",
       " (Seq('TGCCAAACAATACCGATGGTGCAGCTTTGGT'), 1.997696811857739),\n",
       " (Seq('GCCAAACAATACCGATGGTGCAGCTTTGGTT'), 1.997696811857739),\n",
       " (Seq('CCAAACAATACCGATGGTGCAGCTTTGGTTG'), 1.997696811857739),\n",
       " (Seq('CGCTAACGCGAGGTCTGGTCACATCTGAATA'), 1.997696811857739),\n",
       " (Seq('GATTTAGGCTGGCCACCATTGGCCGCAATTA'), 1.997696811857739),\n",
       " (Seq('GATTAATGTCGCCATGCTGGCCCTGAATAAG'), 1.997696811857739),\n",
       " (Seq('TTAATGTCGCCATGCTGGCCCTGAATAAGAC'), 1.997696811857739),\n",
       " (Seq('CATGCTGGCCCTGAATAAGACACGTACTGTT'), 1.997696811857739),\n",
       " (Seq('ATGCTGGCCCTGAATAAGACACGTACTGTTG'), 1.997696811857739),\n",
       " (Seq('TGCTGGCCCTGAATAAGACACGTACTGTTGA'), 1.997696811857739),\n",
       " (Seq('GCTGGCCCTGAATAAGACACGTACTGTTGAT'), 1.997696811857739),\n",
       " (Seq('CTGGCCCTGAATAAGACACGTACTGTTGATG'), 1.997696811857739),\n",
       " (Seq('GGCCCTGAATAAGACACGTACTGTTGATGTC'), 1.997696811857739),\n",
       " (Seq('GCCCTGAATAAGACACGTACTGTTGATGTCG'), 1.997696811857739),\n",
       " (Seq('GAATGCCAAATATCCTGGCCGTGATGATCTC'), 1.997696811857739),\n",
       " (Seq('TATCGCGGTAGCAGTAGGTAACCCAGCCATT'), 1.997696811857739),\n",
       " (Seq('TCGCGGTAGCAGTAGGTAACCCAGCCATTAT'), 1.997696811857739),\n",
       " (Seq('GGCAATATTTTCCGGGCTGTGATAACCGCAA'), 1.997696811857739),\n",
       " (Seq('ATTGTCGCCGACTTGTGGATTGCGACCAAAA'), 1.997696811857739),\n",
       " (Seq('TCGCCGACTTGTGGATTGCGACCAAAATTGC'), 1.997696811857739),\n",
       " (Seq('CGACTTGTGGATTGCGACCAAAATTGCGCCA'), 1.997696811857739),\n",
       " (Seq('GACTTGTGGATTGCGACCAAAATTGCGCCAT'), 1.997696811857739),\n",
       " (Seq('CAGGTATCAAGCTTCCTTGATGGCGGTAACC'), 1.997696811857739),\n",
       " (Seq('AGGTATCAAGCTTCCTTGATGGCGGTAACCC'), 1.997696811857739),\n",
       " (Seq('TTCGCCTGACAAAAGGAACGGTTTATGCCTG'), 1.997696811857739),\n",
       " (Seq('AGGAACGGTTTATGCCTGATTACCACGCAGC'), 1.997696811857739),\n",
       " (Seq('GCAGCGCTTGTCATTGACATTGGCACAACTA'), 1.997696811857739),\n",
       " (Seq('ATAAGGAGGGCGTCATTCTTACCCCGATGCT'), 1.997696811857739),\n",
       " (Seq('CTTATCTCTCGTCATTAAGCAAGGCAGAGGC'), 1.997696811857739),\n",
       " (Seq('TATCTCTCGTCATTAAGCAAGGCAGAGGCTG'), 1.997696811857739),\n",
       " (Seq('GAAATATCCCTGGCCAGTAGAACGTTGTGTC'), 1.997696811857739),\n",
       " (Seq('CAACGCTTAGAGAGGAATTGGGCTTCTCTCA'), 1.997696811857739),\n",
       " (Seq('GGGCTTCTCTCATGAGGTAAAAGTCACACTC'), 1.997696811857739),\n",
       " (Seq('GGCTTCTCTCATGAGGTAAAAGTCACACTCG'), 1.997696811857739),\n",
       " (Seq('TCTCTCATGAGGTAAAAGTCACACTCGCTGG'), 1.997696811857739),\n",
       " (Seq('ATCATATGGTCGGTGCCCGCGCATTACAGAT'), 1.997696811857739),\n",
       " (Seq('CTGAATTCTACCGGCACGACAGAGGGTATTT'), 1.997696811857739),\n",
       " (Seq('TCTACCGGCACGACAGAGGGTATTTTACTGC'), 1.997696811857739),\n",
       " (Seq('CTACCGGCACGACAGAGGGTATTTTACTGCT'), 1.997696811857739),\n",
       " (Seq('TGCGGTGAAAAACCCATACTGGCTCCAGTTG'), 1.997696811857739),\n",
       " (Seq('AGTTGAAGGCTGACATCCTCCAGTGTCCAAT'), 1.997696811857739),\n",
       " (Seq('TGAAGGCTGACATCCTCCAGTGTCCAATTGA'), 1.997696811857739),\n",
       " (Seq('GAAGGCTGACATCCTCCAGTGTCCAATTGAG'), 1.997696811857739),\n",
       " (Seq('AAGGCTGACATCCTCCAGTGTCCAATTGAGG'), 1.997696811857739),\n",
       " (Seq('CCTGACGCGGTTCGTTCTGCAAAGTTGAAAA'), 1.997696811857739),\n",
       " (Seq('ACATGTCCTTGCGGGGGCATTTAATACAACG'), 1.997696811857739),\n",
       " (Seq('CATGTCCTTGCGGGGGCATTTAATACAACGA'), 1.997696811857739),\n",
       " (Seq('TGTCCTTGCGGGGGCATTTAATACAACGAAC'), 1.997696811857739),\n",
       " (Seq('GTCCTTGCGGGGGCATTTAATACAACGAACC'), 1.997696811857739),\n",
       " (Seq('TCCTTGCGGGGGCATTTAATACAACGAACCT'), 1.997696811857739),\n",
       " (Seq('CCTTGCGGGGGCATTTAATACAACGAACCTG'), 1.997696811857739),\n",
       " (Seq('GCCGATAAAATGGATATTCCTGTCAGCCTGC'), 1.997696811857739),\n",
       " (Seq('TCCTGTCAGCCTGCATCTGGATCATGGGAAA'), 1.997696811857739),\n",
       " (Seq('CCTGTCAGCCTGCATCTGGATCATGGGAAAA'), 1.997696811857739),\n",
       " (Seq('CTGTCAGCCTGCATCTGGATCATGGGAAAAC'), 1.997696811857739),\n",
       " (Seq('TGTCAGCCTGCATCTGGATCATGGGAAAACT'), 1.997696811857739),\n",
       " (Seq('GTCAGCCTGCATCTGGATCATGGGAAAACTC'), 1.997696811857739),\n",
       " (Seq('TCAGCCTGCATCTGGATCATGGGAAAACTCT'), 1.997696811857739),\n",
       " (Seq('CAGCCTGCATCTGGATCATGGGAAAACTCTG'), 1.997696811857739),\n",
       " (Seq('AACCGTTGATACGCTGGTATTCAGGTACCGA'), 1.997696811857739),\n",
       " (Seq('CGAAAACCGGCTGGCTAACGTTGAGTTCTTC'), 1.997696811857739),\n",
       " (Seq('TGCTTAAGCAGGCCGTGTCAAAACTGCGCAT'), 1.997696811857739),\n",
       " (Seq('GCTTAAGCAGGCCGTGTCAAAACTGCGCATT'), 1.997696811857739),\n",
       " (Seq('CTTAAGCAGGCCGTGTCAAAACTGCGCATTG'), 1.997696811857739),\n",
       " (Seq('TTAAGCAGGCCGTGTCAAAACTGCGCATTGT'), 1.997696811857739),\n",
       " (Seq('TAAGCAGGCCGTGTCAAAACTGCGCATTGTC'), 1.997696811857739),\n",
       " (Seq('AAGCAGGCCGTGTCAAAACTGCGCATTGTCT'), 1.997696811857739),\n",
       " (Seq('ATTGTCTCCTGGGGAATGGCATCCACGCTAA'), 1.997696811857739),\n",
       " (Seq('TTGTCTCCTGGGGAATGGCATCCACGCTAAA'), 1.997696811857739),\n",
       " (Seq('TGTCTCCTGGGGAATGGCATCCACGCTAAAT'), 1.997696811857739),\n",
       " (Seq('GTCTCCTGGGGAATGGCATCCACGCTAAATA'), 1.997696811857739),\n",
       " (Seq('CAGGCAAGCATAGTGTTTTGCTAACTCACGG'), 1.997696811857739),\n",
       " (Seq('GCATAGTGTTTTGCTAACTCACGGAAACCCG'), 1.997696811857739),\n",
       " (Seq('ATAGTGTTTTGCTAACTCACGGAAACCCGCG'), 1.997696811857739),\n",
       " (Seq('TAGTGTTTTGCTAACTCACGGAAACCCGCGA'), 1.997696811857739),\n",
       " (Seq('AGTGTTTTGCTAACTCACGGAAACCCGCGAG'), 1.997696811857739),\n",
       " (Seq('GTGTTTTGCTAACTCACGGAAACCCGCGAGT'), 1.997696811857739),\n",
       " (Seq('GGAAGGTGTAAAACCAATGCTCGTCTCCCTT'), 1.997696811857739),\n",
       " (Seq('TGCCAACACGATTGCGCGATCGCGATTTTGA'), 1.997696811857739),\n",
       " (Seq('GCCAACACGATTGCGCGATCGCGATTTTGAT'), 1.997696811857739),\n",
       " (Seq('TGCGTGGATTTTCCGCACTAAAACGACGCAG'), 1.997696811857739),\n",
       " (Seq('CGTGGATTTTCCGCACTAAAACGACGCAGGT'), 1.997696811857739),\n",
       " (Seq('CCAGATTATTCAGGCGACCGACAGGCGTTAT'), 1.997696811857739),\n",
       " (Seq('GATTATTCAGGCGACCGACAGGCGTTATATC'), 1.997696811857739),\n",
       " (Seq('ATTATTCAGGCGACCGACAGGCGTTATATCG'), 1.997696811857739),\n",
       " (Seq('ATTCAGGCGACCGACAGGCGTTATATCGTTA'), 1.997696811857739),\n",
       " (Seq('CAGGCGACCGACAGGCGTTATATCGTTATTC'), 1.997696811857739),\n",
       " (Seq('TATGTCCTGAAACGTTCGGTAAAACTGGCGC'), 1.997696811857739),\n",
       " (Seq('ATGTCCTGAAACGTTCGGTAAAACTGGCGCT'), 1.997696811857739),\n",
       " (Seq('CATCCAGGTCGCGGTGATTCAACTGGCAAAT'), 1.997696811857739),\n",
       " (Seq('TCCAGGTCGCGGTGATTCAACTGGCAAATAC'), 1.997696811857739),\n",
       " (Seq('CAGGTCGCGGTGATTCAACTGGCAAATACCT'), 1.997696811857739),\n",
       " (Seq('CCGGATACGACGTTAGTCGTTATCCGGCAAT'), 1.997696811857739),\n",
       " (Seq('CGGATACGACGTTAGTCGTTATCCGGCAATA'), 1.997696811857739),\n",
       " (Seq('TCCCGTTTTCGGAGATAAAACAGCGCGGATT'), 1.997696811857739),\n",
       " (Seq('AACAGCGCGGATTGCCGTAATTATCCCGTAG'), 1.997696811857739),\n",
       " (Seq('CGTAAATTTCCCAGCCGCGGTAAGGGTTCAT'), 1.997696811857739),\n",
       " (Seq('GTAAATTTCCCAGCCGCGGTAAGGGTTCATC'), 1.997696811857739),\n",
       " (Seq('TTGCGCCCCGGCATGTCGTAATAGTCAAATA'), 1.997696811857739),\n",
       " (Seq('CATTTCACGCGGCGCGGCTGATAATAGTTAA'), 1.997696811857739),\n",
       " (Seq('TTCACGCGGCGCGGCTGATAATAGTTAATCC'), 1.997696811857739),\n",
       " (Seq('CGGCGCGGCTGATAATAGTTAATCCCCAGTA'), 1.997696811857739),\n",
       " (Seq('AATCGATTTTGCCGTCGGCAATAAGCTGACG'), 1.997696811857739),\n",
       " (Seq('TGGTCATAGGTTTTCAGCAGCGCCACCAAGT'), 1.997696811857739),\n",
       " (Seq('TTGAATAACAGATCCGCATGATGCGCGGCTT'), 1.997696811857739),\n",
       " (Seq('TTAAAGGTAAACCAGTGCTTCACGCGGTCGC'), 1.997696811857739),\n",
       " (Seq('CTCGTCGATGACGTGATTGTAGAAGCTCACC'), 1.997696811857739),\n",
       " (Seq('GTTTCTGCTGGACCGATGCCCTGATGAAAAC'), 1.997696811857739),\n",
       " (Seq('TTTCTGCTGGACCGATGCCCTGATGAAAACG'), 1.997696811857739),\n",
       " (Seq('TTCTGCTGGACCGATGCCCTGATGAAAACGA'), 1.997696811857739),\n",
       " (Seq('ATCGGGAAAACGGTATCGCATTGTCGTACTC'), 1.997696811857739),\n",
       " (Seq('TCGGGAAAACGGTATCGCATTGTCGTACTCC'), 1.997696811857739),\n",
       " (Seq('CGGGAAAACGGTATCGCATTGTCGTACTCCT'), 1.997696811857739),\n",
       " (Seq('TTGATCGCGTAAATTCGCTCCAGAATACGCG'), 1.997696811857739),\n",
       " (Seq('TGATCGCGTAAATTCGCTCCAGAATACGCGG'), 1.997696811857739),\n",
       " (Seq('GATCGCGTAAATTCGCTCCAGAATACGCGGT'), 1.997696811857739),\n",
       " (Seq('ATCGCGTAAATTCGCTCCAGAATACGCGGTT'), 1.997696811857739),\n",
       " (Seq('AAATTCGCTCCAGAATACGCGGTTTTGGCGA'), 1.997696811857739),\n",
       " (Seq('AATTCGCTCCAGAATACGCGGTTTTGGCGAA'), 1.997696811857739),\n",
       " (Seq('ATTCGCTCCAGAATACGCGGTTTTGGCGAAC'), 1.997696811857739),\n",
       " (Seq('TTCGCTCCAGAATACGCGGTTTTGGCGAACA'), 1.997696811857739),\n",
       " (Seq('TTTTGGCGAACAGAAACCGGTTACAGTCCGC'), 1.997696811857739),\n",
       " (Seq('GAAAAGGTGTGCTACATCTCCGGGCATTCCA'), 1.997696811857739),\n",
       " (Seq('GTAAGTATAGACCGTGCGACGTTCACTCATC'), 1.997696811857739),\n",
       " (Seq('TAAGTATAGACCGTGCGACGTTCACTCATCG'), 1.997696811857739),\n",
       " ...]"
      ]
     },
     "execution_count": 45,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "sorted(kmer_complexity, key=lambda x: x[1], reverse=True)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "id": "923991bf-6a63-4a53-94ae-c563ca58433b",
   "metadata": {},
   "outputs": [],
   "source": []
  }
 ],
 "metadata": {
  "kernelspec": {
   "display_name": "Python 3",
   "language": "python",
   "name": "python3"
  },
  "language_info": {
   "codemirror_mode": {
    "name": "ipython",
    "version": 3
   },
   "file_extension": ".py",
   "mimetype": "text/x-python",
   "name": "python",
   "nbconvert_exporter": "python",
   "pygments_lexer": "ipython3",
   "version": "3.6.13"
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 },
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