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Detail description:
After all the CTSSs are read from the BAM files, is there a possibility to filter out CTSSs that overlap with some genomic regions? For example, some accidental leftover rDNA regions.
While such filtering can happen downstream -- by filtering out tagClusters that overlap the said genomic regions, but keeping them could affect/bias the normalization step. Hence, completely discarding them from the CAGEr object itself would be a better approach, IMO.
The text was updated successfully, but these errors were encountered:
Detail description:
After all the CTSSs are read from the BAM files, is there a possibility to filter out CTSSs that overlap with some genomic regions? For example, some accidental leftover rDNA regions.
While such filtering can happen downstream -- by filtering out tagClusters that overlap the said genomic regions, but keeping them could affect/bias the normalization step. Hence, completely discarding them from the CAGEr object itself would be a better approach, IMO.
The text was updated successfully, but these errors were encountered: