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WGS read-pairs are always from the same segment #31

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cerebis opened this issue Jan 26, 2024 · 0 comments
Open

WGS read-pairs are always from the same segment #31

cerebis opened this issue Jan 26, 2024 · 0 comments

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@cerebis
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cerebis commented Jan 26, 2024

An oversight has been discovered in the simulation of WGS pairs.

Despite the possibility that real WGS pairs can span neighbouring contigs when occurring at contig-ends, during simulation in sim3C, these contig-spanning WGS pairs are never generated.

This is because no assumption is made regarding the orientation and order of non-contiguous sequences, even when defined as a single molecule group.

It would be possible to allow this as part of the community definition.

Two approaches:

  1. stating explicitly that sim3C should consider the multi-fasta as defining the order and orientation
  2. allow the user to specify collective ordering and orientations.

The second option would be more work to implement.

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