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We are running T-Coffee heavily for our (GeneSeqToFamily) workflow and we found possibly a bug in T-Coffee. When we put same input (protein) FASTA file in different way
by using normal input file and (./t_coffee 'temp' -method clustalw_msa -output fasta_aln > direct.fasta)
by standard input (stdin), (cat temp | ./t_coffee '-infile=stdin' -method clustalw_msa -output fasta_aln > indirect.fasta)
resulting alignment varies.
I am attaching input and output files for your reference.
Hi
We are running T-Coffee heavily for our (GeneSeqToFamily) workflow and we found possibly a bug in T-Coffee. When we put same input (protein) FASTA file in different way
./t_coffee 'temp' -method clustalw_msa -output fasta_aln > direct.fasta
)cat temp | ./t_coffee '-infile=stdin' -method clustalw_msa -output fasta_aln > indirect.fasta
)resulting alignment varies.
I am attaching input and output files for your reference.
Archive.zip
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