diff --git a/CHANGELOG.md b/CHANGELOG.md
index beee4e54b..6d61f7762 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -6,17 +6,19 @@
 ### Features
 * Added `get_stream_names` to `OpenEphysRecordingInterface`: [PR #1039](https://github.com/catalystneuro/neuroconv/pull/1039)
 
-### Bug fixes
-
 ### Improvements
 * Using ruff to enforce existence of public classes' docstrings [PR #1034](https://github.com/catalystneuro/neuroconv/pull/1034)
 * Separated tests that use external data by modality [PR #1049](https://github.com/catalystneuro/neuroconv/pull/1049)
 
+
+
 ## v0.6.1 (August 30, 2024)
 
 ### Bug fixes
 * Fixed the JSON schema inference warning on excluded fields; also improved error message reporting of which method triggered the error. [PR #1037](https://github.com/catalystneuro/neuroconv/pull/1037)
 
+
+
 ## v0.6.0 (August 27, 2024)
 
 ### Deprecations
@@ -38,6 +40,7 @@
 * Added helper function `neuroconv.tools.data_transfers.submit_aws_batch_job` for basic automated submission of AWS batch jobs. [PR #384](https://github.com/catalystneuro/neuroconv/pull/384)
 * Data interfaces `run_conversion` method now performs metadata validation before running the conversion. [PR #949](https://github.com/catalystneuro/neuroconv/pull/949)
 * Introduced `null_values_for_properties` to `add_units_table` to give user control over null values behavior [PR #989](https://github.com/catalystneuro/neuroconv/pull/989)
+* Most data interfaces and converters now use Pydantic to validate their inputs, including existence of file and folder paths. [PR #1022](https://github.com/catalystneuro/neuroconv/pull/1022)
 
 ### Bug fixes
 * Fixed the default naming of multiple electrical series in the `SpikeGLXConverterPipe`. [PR #957](https://github.com/catalystneuro/neuroconv/pull/957)
diff --git a/src/neuroconv/basedatainterface.py b/src/neuroconv/basedatainterface.py
index f8fce62b0..59415b043 100644
--- a/src/neuroconv/basedatainterface.py
+++ b/src/neuroconv/basedatainterface.py
@@ -6,7 +6,7 @@
 from typing import Literal, Optional, Union
 
 from jsonschema.validators import validate
-from pydantic import FilePath
+from pydantic import FilePath, validate_call
 from pynwb import NWBFile
 
 from .tools.nwb_helpers import (
@@ -39,6 +39,7 @@ def get_source_schema(cls) -> dict:
         """Infer the JSON schema for the source_data from the method signature (annotation typing)."""
         return get_json_schema_from_method_signature(cls, exclude=["source_data"])
 
+    @validate_call
     def __init__(self, verbose: bool = False, **source_data):
         self.verbose = verbose
         self.source_data = source_data
diff --git a/src/neuroconv/datainterfaces/behavior/audio/audiointerface.py b/src/neuroconv/datainterfaces/behavior/audio/audiointerface.py
index d61cdf18b..fc3f08fb8 100644
--- a/src/neuroconv/datainterfaces/behavior/audio/audiointerface.py
+++ b/src/neuroconv/datainterfaces/behavior/audio/audiointerface.py
@@ -4,7 +4,7 @@
 
 import numpy as np
 import scipy
-from pydantic import FilePath
+from pydantic import FilePath, validate_call
 from pynwb import NWBFile
 
 from ....basetemporalalignmentinterface import BaseTemporalAlignmentInterface
@@ -28,6 +28,7 @@ class AudioInterface(BaseTemporalAlignmentInterface):
     associated_suffixes = (".wav",)
     info = "Interface for writing audio recordings to an NWB file."
 
+    @validate_call
     def __init__(self, file_paths: list[FilePath], verbose: bool = False):
         """
         Data interface for writing acoustic recordings to an NWB file.
diff --git a/src/neuroconv/datainterfaces/behavior/deeplabcut/deeplabcutdatainterface.py b/src/neuroconv/datainterfaces/behavior/deeplabcut/deeplabcutdatainterface.py
index c6850b555..a0719470b 100644
--- a/src/neuroconv/datainterfaces/behavior/deeplabcut/deeplabcutdatainterface.py
+++ b/src/neuroconv/datainterfaces/behavior/deeplabcut/deeplabcutdatainterface.py
@@ -2,7 +2,7 @@
 from typing import Optional, Union
 
 import numpy as np
-from pydantic import FilePath
+from pydantic import FilePath, validate_call
 from pynwb.file import NWBFile
 
 from ....basetemporalalignmentinterface import BaseTemporalAlignmentInterface
@@ -25,6 +25,7 @@ def get_source_schema(cls) -> dict:
         source_schema["properties"]["config_file_path"]["description"] = "Path to .yml config file"
         return source_schema
 
+    @validate_call
     def __init__(
         self,
         file_path: FilePath,
diff --git a/src/neuroconv/datainterfaces/behavior/fictrac/fictracdatainterface.py b/src/neuroconv/datainterfaces/behavior/fictrac/fictracdatainterface.py
index 13136b691..1b9686fd1 100644
--- a/src/neuroconv/datainterfaces/behavior/fictrac/fictracdatainterface.py
+++ b/src/neuroconv/datainterfaces/behavior/fictrac/fictracdatainterface.py
@@ -6,7 +6,7 @@
 from typing import Optional, Union
 
 import numpy as np
-from pydantic import FilePath
+from pydantic import FilePath, validate_call
 from pynwb.behavior import Position, SpatialSeries
 from pynwb.file import NWBFile
 
@@ -154,6 +154,7 @@ def get_source_schema(cls) -> dict:
         source_schema["properties"]["file_path"]["description"] = "Path to the .dat file (the output of fictrac)"
         return source_schema
 
+    @validate_call
     def __init__(
         self,
         file_path: FilePath,
diff --git a/src/neuroconv/datainterfaces/behavior/lightningpose/lightningposeconverter.py b/src/neuroconv/datainterfaces/behavior/lightningpose/lightningposeconverter.py
index 114cff0dd..dee848f19 100644
--- a/src/neuroconv/datainterfaces/behavior/lightningpose/lightningposeconverter.py
+++ b/src/neuroconv/datainterfaces/behavior/lightningpose/lightningposeconverter.py
@@ -1,7 +1,7 @@
 from copy import deepcopy
 from typing import Optional
 
-from pydantic import FilePath
+from pydantic import FilePath, validate_call
 from pynwb import NWBFile
 
 from neuroconv import NWBConverter
@@ -26,6 +26,7 @@ class LightningPoseConverter(NWBConverter):
     def get_source_schema(cls):
         return get_schema_from_method_signature(cls)
 
+    @validate_call
     def __init__(
         self,
         file_path: FilePath,
diff --git a/src/neuroconv/datainterfaces/behavior/lightningpose/lightningposedatainterface.py b/src/neuroconv/datainterfaces/behavior/lightningpose/lightningposedatainterface.py
index b87366109..f103b7c9a 100644
--- a/src/neuroconv/datainterfaces/behavior/lightningpose/lightningposedatainterface.py
+++ b/src/neuroconv/datainterfaces/behavior/lightningpose/lightningposedatainterface.py
@@ -5,7 +5,7 @@
 from typing import Optional
 
 import numpy as np
-from pydantic import FilePath
+from pydantic import FilePath, validate_call
 from pynwb import NWBFile
 
 from ....basetemporalalignmentinterface import BaseTemporalAlignmentInterface
@@ -58,6 +58,7 @@ def get_metadata_schema(self) -> dict:
 
         return metadata_schema
 
+    @validate_call
     def __init__(
         self,
         file_path: FilePath,
diff --git a/src/neuroconv/datainterfaces/behavior/medpc/medpcdatainterface.py b/src/neuroconv/datainterfaces/behavior/medpc/medpcdatainterface.py
index 0c109f559..6a4127663 100644
--- a/src/neuroconv/datainterfaces/behavior/medpc/medpcdatainterface.py
+++ b/src/neuroconv/datainterfaces/behavior/medpc/medpcdatainterface.py
@@ -1,7 +1,7 @@
 from typing import Optional
 
 import numpy as np
-from pydantic import FilePath
+from pydantic import FilePath, validate_call
 from pynwb.behavior import BehavioralEpochs, IntervalSeries
 from pynwb.file import NWBFile
 
@@ -41,6 +41,7 @@ class MedPCInterface(BaseTemporalAlignmentInterface):
     info = "Interface for handling MedPC output files."
     associated_suffixes = (".txt",)
 
+    @validate_call
     def __init__(
         self,
         file_path: FilePath,
diff --git a/src/neuroconv/datainterfaces/behavior/miniscope/miniscopedatainterface.py b/src/neuroconv/datainterfaces/behavior/miniscope/miniscopedatainterface.py
index 6a46e84fe..ecb763523 100644
--- a/src/neuroconv/datainterfaces/behavior/miniscope/miniscopedatainterface.py
+++ b/src/neuroconv/datainterfaces/behavior/miniscope/miniscopedatainterface.py
@@ -1,6 +1,6 @@
 from pathlib import Path
 
-from pydantic import DirectoryPath
+from pydantic import DirectoryPath, validate_call
 from pynwb import NWBFile
 
 from .... import BaseDataInterface
@@ -24,6 +24,7 @@ def get_source_schema(cls) -> dict:
         ] = "The main Miniscope folder. The movie files are expected to be in sub folders within the main folder."
         return source_schema
 
+    @validate_call
     def __init__(self, folder_path: DirectoryPath):
         """
         Initialize reading recordings from the Miniscope behavioral camera.
diff --git a/src/neuroconv/datainterfaces/behavior/neuralynx/neuralynx_nvt_interface.py b/src/neuroconv/datainterfaces/behavior/neuralynx/neuralynx_nvt_interface.py
index f01d11e53..e161387f0 100644
--- a/src/neuroconv/datainterfaces/behavior/neuralynx/neuralynx_nvt_interface.py
+++ b/src/neuroconv/datainterfaces/behavior/neuralynx/neuralynx_nvt_interface.py
@@ -2,7 +2,7 @@
 from typing import Optional
 
 import numpy as np
-from pydantic import FilePath
+from pydantic import FilePath, validate_call
 from pynwb import NWBFile
 from pynwb.behavior import CompassDirection, Position, SpatialSeries
 
@@ -20,6 +20,7 @@ class NeuralynxNvtInterface(BaseTemporalAlignmentInterface):
     associated_suffixes = (".nvt",)
     info = "Interface for writing Neuralynx position tracking .nvt files to NWB."
 
+    @validate_call
     def __init__(self, file_path: FilePath, verbose: bool = True):
         """
         Interface for writing Neuralynx .nvt files to nwb.
diff --git a/src/neuroconv/datainterfaces/behavior/sleap/sleapdatainterface.py b/src/neuroconv/datainterfaces/behavior/sleap/sleapdatainterface.py
index a74b90a67..713b21c98 100644
--- a/src/neuroconv/datainterfaces/behavior/sleap/sleapdatainterface.py
+++ b/src/neuroconv/datainterfaces/behavior/sleap/sleapdatainterface.py
@@ -2,7 +2,7 @@
 from typing import Optional
 
 import numpy as np
-from pydantic import FilePath
+from pydantic import FilePath, validate_call
 from pynwb.file import NWBFile
 
 from .sleap_utils import extract_timestamps
@@ -27,6 +27,7 @@ def get_source_schema(cls) -> dict:
         ] = "Path of the video for extracting timestamps (optional)."
         return source_schema
 
+    @validate_call
     def __init__(
         self,
         file_path: FilePath,
diff --git a/src/neuroconv/datainterfaces/behavior/video/videodatainterface.py b/src/neuroconv/datainterfaces/behavior/video/videodatainterface.py
index dfb22deba..7a28c2d2f 100644
--- a/src/neuroconv/datainterfaces/behavior/video/videodatainterface.py
+++ b/src/neuroconv/datainterfaces/behavior/video/videodatainterface.py
@@ -6,7 +6,7 @@
 import numpy as np
 import psutil
 from hdmf.data_utils import DataChunkIterator
-from pydantic import FilePath
+from pydantic import FilePath, validate_call
 from pynwb import NWBFile
 from pynwb.image import ImageSeries
 from tqdm import tqdm
@@ -28,6 +28,7 @@ class VideoInterface(BaseDataInterface):
     # Other suffixes, while they can be opened by OpenCV, are not supported by DANDI so should probably not list here
     info = "Interface for handling standard video file formats."
 
+    @validate_call
     def __init__(
         self,
         file_paths: list[FilePath],
diff --git a/src/neuroconv/datainterfaces/ecephys/openephys/openephyslegacydatainterface.py b/src/neuroconv/datainterfaces/ecephys/openephys/openephyslegacydatainterface.py
index 0818257a7..b3392d2db 100644
--- a/src/neuroconv/datainterfaces/ecephys/openephys/openephyslegacydatainterface.py
+++ b/src/neuroconv/datainterfaces/ecephys/openephys/openephyslegacydatainterface.py
@@ -2,7 +2,7 @@
 from typing import Optional
 from warnings import warn
 
-from pydantic import DirectoryPath
+from pydantic import DirectoryPath, validate_call
 
 from ..baserecordingextractorinterface import BaseRecordingExtractorInterface
 
@@ -35,6 +35,7 @@ def get_source_schema(cls):
 
         return source_schema
 
+    @validate_call
     def __init__(
         self,
         folder_path: DirectoryPath,
diff --git a/src/neuroconv/datainterfaces/ecephys/openephys/openephyssortingdatainterface.py b/src/neuroconv/datainterfaces/ecephys/openephys/openephyssortingdatainterface.py
index 20f292c9a..2d53e6331 100644
--- a/src/neuroconv/datainterfaces/ecephys/openephys/openephyssortingdatainterface.py
+++ b/src/neuroconv/datainterfaces/ecephys/openephys/openephyssortingdatainterface.py
@@ -1,4 +1,4 @@
-from pydantic import DirectoryPath
+from pydantic import DirectoryPath, validate_call
 
 from ..basesortingextractorinterface import BaseSortingExtractorInterface
 from ....utils import get_schema_from_method_signature
@@ -23,6 +23,7 @@ def get_source_schema(cls) -> dict:
         metadata_schema["additionalProperties"] = False
         return metadata_schema
 
+    @validate_call
     def __init__(self, folder_path: DirectoryPath, experiment_id: int = 0, recording_id: int = 0):
         from spikeextractors import OpenEphysSortingExtractor
 
diff --git a/src/neuroconv/datainterfaces/ecephys/phy/phydatainterface.py b/src/neuroconv/datainterfaces/ecephys/phy/phydatainterface.py
index da5ebbfc2..07324b602 100644
--- a/src/neuroconv/datainterfaces/ecephys/phy/phydatainterface.py
+++ b/src/neuroconv/datainterfaces/ecephys/phy/phydatainterface.py
@@ -1,6 +1,6 @@
 from typing import Optional
 
-from pydantic import DirectoryPath
+from pydantic import DirectoryPath, validate_call
 
 from ..basesortingextractorinterface import BaseSortingExtractorInterface
 
@@ -24,6 +24,7 @@ def get_source_schema(cls) -> dict:
         ] = "Path to the output Phy folder (containing the params.py)."
         return source_schema
 
+    @validate_call
     def __init__(
         self,
         folder_path: DirectoryPath,
diff --git a/src/neuroconv/datainterfaces/ecephys/plexon/plexondatainterface.py b/src/neuroconv/datainterfaces/ecephys/plexon/plexondatainterface.py
index 8b2e53338..4a6a50fa5 100644
--- a/src/neuroconv/datainterfaces/ecephys/plexon/plexondatainterface.py
+++ b/src/neuroconv/datainterfaces/ecephys/plexon/plexondatainterface.py
@@ -1,6 +1,6 @@
 from pathlib import Path
 
-from pydantic import FilePath
+from pydantic import FilePath, validate_call
 
 from ..baserecordingextractorinterface import BaseRecordingExtractorInterface
 from ..basesortingextractorinterface import BaseSortingExtractorInterface
@@ -24,6 +24,7 @@ def get_source_schema(cls) -> dict:
         source_schema["properties"]["file_path"]["description"] = "Path to the .plx file."
         return source_schema
 
+    @validate_call
     def __init__(self, file_path: FilePath, verbose: bool = True, es_key: str = "ElectricalSeries"):
         """
         Load and prepare data for Plexon.
@@ -68,6 +69,7 @@ def get_source_schema(cls) -> dict:
         source_schema["properties"]["file_path"]["description"] = "Path to the .pl2 file."
         return source_schema
 
+    @validate_call
     def __init__(self, file_path: FilePath, verbose: bool = True, es_key: str = "ElectricalSeries"):
         """
         Load and prepare data for Plexon.
@@ -119,6 +121,7 @@ def get_source_schema(cls) -> dict:
         source_schema["properties"]["file_path"]["description"] = "Path to the plexon spiking data (.plx file)."
         return source_schema
 
+    @validate_call
     def __init__(self, file_path: FilePath, verbose: bool = True):
         """
         Load and prepare data for Plexon.
diff --git a/src/neuroconv/datainterfaces/ecephys/spike2/spike2datainterface.py b/src/neuroconv/datainterfaces/ecephys/spike2/spike2datainterface.py
index 5895dfe3c..ccd98a369 100644
--- a/src/neuroconv/datainterfaces/ecephys/spike2/spike2datainterface.py
+++ b/src/neuroconv/datainterfaces/ecephys/spike2/spike2datainterface.py
@@ -1,6 +1,6 @@
 from pathlib import Path
 
-from pydantic import FilePath
+from pydantic import FilePath, validate_call
 
 from ..baserecordingextractorinterface import BaseRecordingExtractorInterface
 from ....tools import get_package
@@ -40,6 +40,7 @@ def get_all_channels_info(cls, file_path: FilePath):
         _test_sonpy_installation()
         return cls.get_extractor().get_all_channels_info(file_path=file_path)
 
+    @validate_call
     def __init__(self, file_path: FilePath, verbose: bool = True, es_key: str = "ElectricalSeries"):
         """
         Initialize reading of Spike2 file.
diff --git a/src/neuroconv/datainterfaces/ecephys/spikegadgets/spikegadgetsdatainterface.py b/src/neuroconv/datainterfaces/ecephys/spikegadgets/spikegadgetsdatainterface.py
index a5ad98d52..4a63dc237 100644
--- a/src/neuroconv/datainterfaces/ecephys/spikegadgets/spikegadgetsdatainterface.py
+++ b/src/neuroconv/datainterfaces/ecephys/spikegadgets/spikegadgetsdatainterface.py
@@ -22,6 +22,7 @@ def get_source_schema(cls) -> dict:
         source_schema["properties"]["file_path"].update(description="Path to SpikeGadgets (.rec) file.")
         return source_schema
 
+    # @validate_call
     def __init__(
         self,
         file_path: FilePath,
diff --git a/src/neuroconv/datainterfaces/ecephys/spikeglx/spikeglxconverter.py b/src/neuroconv/datainterfaces/ecephys/spikeglx/spikeglxconverter.py
index 9d40cde3d..6aeb36cec 100644
--- a/src/neuroconv/datainterfaces/ecephys/spikeglx/spikeglxconverter.py
+++ b/src/neuroconv/datainterfaces/ecephys/spikeglx/spikeglxconverter.py
@@ -1,7 +1,7 @@
 from pathlib import Path
 from typing import Optional
 
-from pydantic import DirectoryPath
+from pydantic import DirectoryPath, validate_call
 
 from .spikeglxdatainterface import SpikeGLXRecordingInterface
 from .spikeglxnidqinterface import SpikeGLXNIDQInterface
@@ -33,6 +33,7 @@ def get_streams(cls, folder_path: DirectoryPath) -> list[str]:
 
         return SpikeGLXRecordingExtractor.get_streams(folder_path=folder_path)[0]
 
+    @validate_call
     def __init__(
         self,
         folder_path: DirectoryPath,
diff --git a/src/neuroconv/datainterfaces/ecephys/spikeglx/spikeglxdatainterface.py b/src/neuroconv/datainterfaces/ecephys/spikeglx/spikeglxdatainterface.py
index 7d97f7d25..d45a7f946 100644
--- a/src/neuroconv/datainterfaces/ecephys/spikeglx/spikeglxdatainterface.py
+++ b/src/neuroconv/datainterfaces/ecephys/spikeglx/spikeglxdatainterface.py
@@ -4,7 +4,7 @@
 from typing import Optional
 
 import numpy as np
-from pydantic import FilePath
+from pydantic import FilePath, validate_call
 
 from .spikeglx_utils import (
     add_recording_extractor_properties,
@@ -42,6 +42,7 @@ def get_source_schema(cls) -> dict:
         source_schema["properties"]["file_path"]["description"] = "Path to SpikeGLX ap.bin or lf.bin file."
         return source_schema
 
+    @validate_call
     def __init__(
         self,
         file_path: FilePath,
diff --git a/src/neuroconv/datainterfaces/ecephys/spikeglx/spikeglxnidqinterface.py b/src/neuroconv/datainterfaces/ecephys/spikeglx/spikeglxnidqinterface.py
index 3c0b886ec..9e903f3f1 100644
--- a/src/neuroconv/datainterfaces/ecephys/spikeglx/spikeglxnidqinterface.py
+++ b/src/neuroconv/datainterfaces/ecephys/spikeglx/spikeglxnidqinterface.py
@@ -25,6 +25,7 @@ def get_source_schema(cls) -> dict:
         source_schema["properties"]["file_path"]["description"] = "Path to SpikeGLX .nidq file."
         return source_schema
 
+    # @validate_call
     def __init__(
         self,
         file_path: FilePath,
diff --git a/src/neuroconv/datainterfaces/ecephys/tdt/tdtdatainterface.py b/src/neuroconv/datainterfaces/ecephys/tdt/tdtdatainterface.py
index 420d0f242..d01e63c5d 100644
--- a/src/neuroconv/datainterfaces/ecephys/tdt/tdtdatainterface.py
+++ b/src/neuroconv/datainterfaces/ecephys/tdt/tdtdatainterface.py
@@ -1,4 +1,4 @@
-from pydantic import DirectoryPath
+from pydantic import DirectoryPath, validate_call
 
 from ..baserecordingextractorinterface import BaseRecordingExtractorInterface
 
@@ -10,6 +10,7 @@ class TdtRecordingInterface(BaseRecordingExtractorInterface):
     associated_suffixes = (".tbk", ".tbx", ".tev", ".tsq")
     info = "Interface for TDT recording data."
 
+    @validate_call
     def __init__(
         self,
         folder_path: DirectoryPath,
diff --git a/src/neuroconv/datainterfaces/icephys/abf/abfdatainterface.py b/src/neuroconv/datainterfaces/icephys/abf/abfdatainterface.py
index 5336b6116..535381466 100644
--- a/src/neuroconv/datainterfaces/icephys/abf/abfdatainterface.py
+++ b/src/neuroconv/datainterfaces/icephys/abf/abfdatainterface.py
@@ -1,9 +1,10 @@
 import json
 from datetime import datetime, timedelta
 from pathlib import Path
+from typing import Optional
 from warnings import warn
 
-from pydantic import FilePath
+from pydantic import FilePath, validate_call
 
 from ..baseicephysinterface import BaseIcephysInterface
 
@@ -50,8 +51,12 @@ def get_source_schema(cls) -> dict:
         )
         return source_schema
 
+    @validate_call
     def __init__(
-        self, file_paths: list[FilePath], icephys_metadata: dict = None, icephys_metadata_file_path: FilePath = None
+        self,
+        file_paths: list[FilePath],
+        icephys_metadata: Optional[dict] = None,
+        icephys_metadata_file_path: Optional[FilePath] = None,
     ):
         """
         ABF IcephysInterface based on Neo AxonIO.
diff --git a/src/neuroconv/datainterfaces/icephys/baseicephysinterface.py b/src/neuroconv/datainterfaces/icephys/baseicephysinterface.py
index ff82ba391..76c9bf840 100644
--- a/src/neuroconv/datainterfaces/icephys/baseicephysinterface.py
+++ b/src/neuroconv/datainterfaces/icephys/baseicephysinterface.py
@@ -1,6 +1,7 @@
 import importlib.util
 
 import numpy as np
+from pydantic import FilePath, validate_call
 from pynwb import NWBFile
 
 from ...baseextractorinterface import BaseExtractorInterface
@@ -24,7 +25,8 @@ def get_source_schema(cls) -> dict:
         source_schema = get_schema_from_method_signature(method=cls.__init__, exclude=[])
         return source_schema
 
-    def __init__(self, file_paths: list):
+    @validate_call
+    def __init__(self, file_paths: list[FilePath]):
         # Check if the ndx_dandi_icephys module is available
         dandi_icephys_spec = importlib.util.find_spec("ndx_dandi_icephys")
         if dandi_icephys_spec is not None:
diff --git a/src/neuroconv/datainterfaces/ophys/hdf5/hdf5datainterface.py b/src/neuroconv/datainterfaces/ophys/hdf5/hdf5datainterface.py
index c980fcf41..2526f7fb3 100644
--- a/src/neuroconv/datainterfaces/ophys/hdf5/hdf5datainterface.py
+++ b/src/neuroconv/datainterfaces/ophys/hdf5/hdf5datainterface.py
@@ -13,6 +13,7 @@ class Hdf5ImagingInterface(BaseImagingExtractorInterface):
     associated_suffixes = (".h5", ".hdf5")
     info = "Interface for HDF5 imaging data."
 
+    # @validate_call
     def __init__(
         self,
         file_path: FilePath,
diff --git a/src/neuroconv/datainterfaces/ophys/micromanagertiff/micromanagertiffdatainterface.py b/src/neuroconv/datainterfaces/ophys/micromanagertiff/micromanagertiffdatainterface.py
index 4e758e742..17cbc95ed 100644
--- a/src/neuroconv/datainterfaces/ophys/micromanagertiff/micromanagertiffdatainterface.py
+++ b/src/neuroconv/datainterfaces/ophys/micromanagertiff/micromanagertiffdatainterface.py
@@ -1,5 +1,5 @@
 from dateutil.parser import parse
-from pydantic import DirectoryPath
+from pydantic import DirectoryPath, validate_call
 
 from ..baseimagingextractorinterface import BaseImagingExtractorInterface
 
@@ -18,6 +18,7 @@ def get_source_schema(cls) -> dict:
         source_schema["properties"]["folder_path"]["description"] = "The folder containing the OME-TIF image files."
         return source_schema
 
+    @validate_call
     def __init__(self, folder_path: DirectoryPath, verbose: bool = True):
         """
         Data Interface for MicroManagerTiffImagingExtractor.
diff --git a/src/neuroconv/datainterfaces/ophys/miniscope/miniscopeconverter.py b/src/neuroconv/datainterfaces/ophys/miniscope/miniscopeconverter.py
index 09aba9f0e..cfee8f027 100644
--- a/src/neuroconv/datainterfaces/ophys/miniscope/miniscopeconverter.py
+++ b/src/neuroconv/datainterfaces/ophys/miniscope/miniscopeconverter.py
@@ -1,6 +1,6 @@
 from typing import Optional
 
-from pydantic import DirectoryPath
+from pydantic import DirectoryPath, validate_call
 from pynwb import NWBFile
 
 from ... import MiniscopeBehaviorInterface, MiniscopeImagingInterface
@@ -23,6 +23,7 @@ def get_source_schema(cls):
         source_schema["properties"]["folder_path"]["description"] = "The path to the main Miniscope folder."
         return source_schema
 
+    @validate_call
     def __init__(self, folder_path: DirectoryPath, verbose: bool = True):
         """
         Initializes the data interfaces for the Miniscope recording and behavioral data stream.
diff --git a/src/neuroconv/datainterfaces/ophys/miniscope/miniscopeimagingdatainterface.py b/src/neuroconv/datainterfaces/ophys/miniscope/miniscopeimagingdatainterface.py
index 594bf00dc..64a180c46 100644
--- a/src/neuroconv/datainterfaces/ophys/miniscope/miniscopeimagingdatainterface.py
+++ b/src/neuroconv/datainterfaces/ophys/miniscope/miniscopeimagingdatainterface.py
@@ -3,7 +3,7 @@
 from typing import Literal, Optional
 
 import numpy as np
-from pydantic import DirectoryPath
+from pydantic import DirectoryPath, validate_call
 from pynwb import NWBFile
 
 from ..baseimagingextractorinterface import BaseImagingExtractorInterface
@@ -26,6 +26,7 @@ def get_source_schema(cls) -> dict:
 
         return source_schema
 
+    @validate_call
     def __init__(self, folder_path: DirectoryPath):
         """
         Initialize reading the Miniscope imaging data.
diff --git a/src/neuroconv/datainterfaces/ophys/sbx/sbxdatainterface.py b/src/neuroconv/datainterfaces/ophys/sbx/sbxdatainterface.py
index 2fa90a2bb..5b921f6f3 100644
--- a/src/neuroconv/datainterfaces/ophys/sbx/sbxdatainterface.py
+++ b/src/neuroconv/datainterfaces/ophys/sbx/sbxdatainterface.py
@@ -1,6 +1,6 @@
 from typing import Literal
 
-from pydantic import FilePath
+from pydantic import FilePath, validate_call
 
 from ..baseimagingextractorinterface import BaseImagingExtractorInterface
 
@@ -12,6 +12,7 @@ class SbxImagingInterface(BaseImagingExtractorInterface):
     associated_suffixes = (".sbx",)
     info = "Interface for Scanbox imaging data."
 
+    @validate_call
     def __init__(
         self,
         file_path: FilePath,
diff --git a/src/neuroconv/datainterfaces/ophys/scanimage/scanimageimaginginterfaces.py b/src/neuroconv/datainterfaces/ophys/scanimage/scanimageimaginginterfaces.py
index 76a67ef9b..09e8f86d3 100644
--- a/src/neuroconv/datainterfaces/ophys/scanimage/scanimageimaginginterfaces.py
+++ b/src/neuroconv/datainterfaces/ophys/scanimage/scanimageimaginginterfaces.py
@@ -4,7 +4,7 @@
 from typing import Optional
 
 from dateutil.parser import parse as dateparse
-from pydantic import DirectoryPath, FilePath
+from pydantic import DirectoryPath, FilePath, validate_call
 
 from ..baseimagingextractorinterface import BaseImagingExtractorInterface
 
@@ -32,6 +32,7 @@ def get_source_schema(cls) -> dict:
         source_schema["properties"]["file_path"]["description"] = "Path to Tiff file."
         return source_schema
 
+    @validate_call
     def __new__(
         cls,
         file_path: FilePath,
@@ -90,6 +91,7 @@ def get_source_schema(cls) -> dict:
         source_schema["properties"]["file_path"]["description"] = "Path to Tiff file."
         return source_schema
 
+    @validate_call
     def __init__(
         self,
         file_path: FilePath,
@@ -168,6 +170,7 @@ def get_source_schema(cls) -> dict:
         source_schema["properties"]["folder_path"]["description"] = "Path to the folder containing the TIFF files."
         return source_schema
 
+    @validate_call
     def __new__(
         cls,
         folder_path: DirectoryPath,
@@ -226,6 +229,7 @@ class ScanImageMultiPlaneImagingInterface(BaseImagingExtractorInterface):
 
     ExtractorName = "ScanImageTiffMultiPlaneImagingExtractor"
 
+    @validate_call
     def __init__(
         self,
         file_path: FilePath,
@@ -327,6 +331,7 @@ class ScanImageMultiPlaneMultiFileImagingInterface(BaseImagingExtractorInterface
 
     ExtractorName = "ScanImageTiffMultiPlaneMultiFileImagingExtractor"
 
+    @validate_call
     def __init__(
         self,
         folder_path: DirectoryPath,
@@ -443,6 +448,7 @@ class ScanImageSinglePlaneImagingInterface(BaseImagingExtractorInterface):
 
     ExtractorName = "ScanImageTiffSinglePlaneImagingExtractor"
 
+    @validate_call
     def __init__(
         self,
         file_path: FilePath,
@@ -560,6 +566,7 @@ class ScanImageSinglePlaneMultiFileImagingInterface(BaseImagingExtractorInterfac
 
     ExtractorName = "ScanImageTiffSinglePlaneMultiFileImagingExtractor"
 
+    @validate_call
     def __init__(
         self,
         folder_path: DirectoryPath,
diff --git a/src/neuroconv/datainterfaces/ophys/sima/simadatainterface.py b/src/neuroconv/datainterfaces/ophys/sima/simadatainterface.py
index 8bf34cc04..570e2cfe3 100644
--- a/src/neuroconv/datainterfaces/ophys/sima/simadatainterface.py
+++ b/src/neuroconv/datainterfaces/ophys/sima/simadatainterface.py
@@ -1,4 +1,4 @@
-from pydantic import FilePath
+from pydantic import FilePath, validate_call
 
 from ..basesegmentationextractorinterface import BaseSegmentationExtractorInterface
 
@@ -10,6 +10,7 @@ class SimaSegmentationInterface(BaseSegmentationExtractorInterface):
     associated_suffixes = (".sima",)
     info = "Interface for SIMA segmentation."
 
+    @validate_call
     def __init__(self, file_path: FilePath, sima_segmentation_label: str = "auto_ROIs"):
         """
 
diff --git a/src/neuroconv/datainterfaces/ophys/suite2p/suite2pdatainterface.py b/src/neuroconv/datainterfaces/ophys/suite2p/suite2pdatainterface.py
index 359dea25f..056616ce5 100644
--- a/src/neuroconv/datainterfaces/ophys/suite2p/suite2pdatainterface.py
+++ b/src/neuroconv/datainterfaces/ophys/suite2p/suite2pdatainterface.py
@@ -1,7 +1,7 @@
 from copy import deepcopy
 from typing import Optional
 
-from pydantic import DirectoryPath
+from pydantic import DirectoryPath, validate_call
 from pynwb import NWBFile
 
 from ..basesegmentationextractorinterface import BaseSegmentationExtractorInterface
@@ -72,6 +72,7 @@ def get_available_channels(cls, folder_path: DirectoryPath) -> dict:
 
         return Suite2pSegmentationExtractor.get_available_channels(folder_path=folder_path)
 
+    @validate_call
     def __init__(
         self,
         folder_path: DirectoryPath,
diff --git a/src/neuroconv/datainterfaces/ophys/tdt_fp/tdtfiberphotometrydatainterface.py b/src/neuroconv/datainterfaces/ophys/tdt_fp/tdtfiberphotometrydatainterface.py
index 72c854634..aa58f6ae4 100644
--- a/src/neuroconv/datainterfaces/ophys/tdt_fp/tdtfiberphotometrydatainterface.py
+++ b/src/neuroconv/datainterfaces/ophys/tdt_fp/tdtfiberphotometrydatainterface.py
@@ -6,7 +6,7 @@
 
 import numpy as np
 import pytz
-from pydantic import FilePath
+from pydantic import DirectoryPath, validate_call
 from pynwb.file import NWBFile
 
 from neuroconv.basetemporalalignmentinterface import BaseTemporalAlignmentInterface
@@ -28,7 +28,8 @@ class TDTFiberPhotometryInterface(BaseTemporalAlignmentInterface):
     info = "Data Interface for converting fiber photometry data from TDT files."
     associated_suffixes = ("Tbk", "Tdx", "tev", "tin", "tsq")
 
-    def __init__(self, folder_path: FilePath, verbose: bool = True):
+    @validate_call
+    def __init__(self, folder_path: DirectoryPath, verbose: bool = True):
         """Initialize the TDTFiberPhotometryInterface.
 
         Parameters
diff --git a/src/neuroconv/datainterfaces/ophys/tiff/tiffdatainterface.py b/src/neuroconv/datainterfaces/ophys/tiff/tiffdatainterface.py
index 017aa2e98..1eaa3b55e 100644
--- a/src/neuroconv/datainterfaces/ophys/tiff/tiffdatainterface.py
+++ b/src/neuroconv/datainterfaces/ophys/tiff/tiffdatainterface.py
@@ -1,6 +1,6 @@
 from typing import Literal
 
-from pydantic import FilePath
+from pydantic import FilePath, validate_call
 
 from ..baseimagingextractorinterface import BaseImagingExtractorInterface
 
@@ -18,6 +18,7 @@ def get_source_schema(cls) -> dict:
         source_schema["properties"]["file_path"]["description"] = "Path to Tiff file."
         return source_schema
 
+    @validate_call
     def __init__(
         self,
         file_path: FilePath,
diff --git a/src/neuroconv/datainterfaces/text/excel/exceltimeintervalsinterface.py b/src/neuroconv/datainterfaces/text/excel/exceltimeintervalsinterface.py
index cb4ae2330..92fbbbaa7 100644
--- a/src/neuroconv/datainterfaces/text/excel/exceltimeintervalsinterface.py
+++ b/src/neuroconv/datainterfaces/text/excel/exceltimeintervalsinterface.py
@@ -1,7 +1,7 @@
 from typing import Optional
 
 import pandas as pd
-from pydantic import FilePath
+from pydantic import FilePath, validate_call
 
 from ..timeintervalsinterface import TimeIntervalsInterface
 
@@ -13,6 +13,7 @@ class ExcelTimeIntervalsInterface(TimeIntervalsInterface):
     associated_suffixes = (".xlsx", ".xls", ".xlsm")
     info = "Interface for writing a time intervals table from an excel file."
 
+    @validate_call
     def __init__(
         self,
         file_path: FilePath,
diff --git a/src/neuroconv/datainterfaces/text/timeintervalsinterface.py b/src/neuroconv/datainterfaces/text/timeintervalsinterface.py
index b00779cb0..5f5b1107d 100644
--- a/src/neuroconv/datainterfaces/text/timeintervalsinterface.py
+++ b/src/neuroconv/datainterfaces/text/timeintervalsinterface.py
@@ -3,7 +3,7 @@
 from typing import Optional
 
 import numpy as np
-from pydantic import FilePath
+from pydantic import FilePath, validate_call
 from pynwb import NWBFile
 
 from ...basedatainterface import BaseDataInterface
@@ -16,6 +16,7 @@ class TimeIntervalsInterface(BaseDataInterface):
 
     keywords = ("table", "trials", "epochs", "time intervals")
 
+    @validate_call
     def __init__(
         self,
         file_path: FilePath,
diff --git a/src/neuroconv/nwbconverter.py b/src/neuroconv/nwbconverter.py
index eabf6d772..1f3e7c9f8 100644
--- a/src/neuroconv/nwbconverter.py
+++ b/src/neuroconv/nwbconverter.py
@@ -7,7 +7,7 @@
 from typing import Literal, Optional, Union
 
 from jsonschema import validate
-from pydantic import FilePath
+from pydantic import FilePath, validate_call
 from pynwb import NWBFile
 
 from .basedatainterface import BaseDataInterface
@@ -73,6 +73,7 @@ def _validate_source_data(self, source_data: dict[str, dict], verbose: bool = Tr
         if verbose:
             print("Source data is valid!")
 
+    @validate_call
     def __init__(self, source_data: dict[str, dict], verbose: bool = True):
         """Validate source_data against source_schema and initialize all data interfaces."""
         self.verbose = verbose
diff --git a/tests/test_behavior/test_audio_interface.py b/tests/test_behavior/test_audio_interface.py
index 4fad4fe3d..50331d744 100644
--- a/tests/test_behavior/test_audio_interface.py
+++ b/tests/test_behavior/test_audio_interface.py
@@ -80,11 +80,6 @@ class AudioTestNWBConverter(NWBConverter):
             aligned_segment_starting_times=self.aligned_segment_starting_times
         )
 
-    def test_unsupported_format(self):
-        exc_msg = "The currently supported file format for audio is WAV file. Some of the provided files does not match this format: ['.test']."
-        with pytest.raises(ValueError, match=re.escape(exc_msg)):
-            AudioInterface(file_paths=["test.test"])
-
     def test_get_metadata(self):
         audio_interface = AudioInterface(file_paths=self.file_paths)
         metadata = audio_interface.get_metadata()
diff --git a/tests/test_on_data/ophys/test_fiber_photometry_interfaces.py b/tests/test_on_data/ophys/test_fiber_photometry_interfaces.py
index b9121f08b..82cbb0e4a 100644
--- a/tests/test_on_data/ophys/test_fiber_photometry_interfaces.py
+++ b/tests/test_on_data/ophys/test_fiber_photometry_interfaces.py
@@ -400,8 +400,3 @@ def test_load_invalid_evtype(self):
         interface = self.data_interface_cls(**self.interface_kwargs)
         with self.assertRaises(AssertionError):
             interface.load(t2=1.0, evtype=["invalid"])
-
-    def test_load_invalid_folder_path(self):
-        interface = self.data_interface_cls(folder_path="invalid")
-        with self.assertRaises(AssertionError):
-            interface.load(t2=1.0)