diff --git a/CHANGELOG.md b/CHANGELOG.md
index c42f77a74..7c6da32f0 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -35,6 +35,7 @@
 * Implemented format-wise and modality-wise extra installation requirements. If there are any requirements to use a module or data interface, these are defined in individual requirements files at the corresponding level of the package. These are in turn easily accessible from the commands `pip install neuroconv[format_name]`. `pip install neuroconv[modality_name]` will also install all dependencies necessary to make full use of any interfaces from that modality. [PR #100](https://github.com/catalystneuro/neuroconv/pull/100)
 * Added frame stubbing to the `BaseSegmentationExtractorInterface`. [PR #116](https://github.com/catalystneuro/neuroconv/pull/116)
 * Added `mask_type: str` and `include_roi_centroids: bool` to the `add_plane_segmentation` helper and `write_segmentation` functions for the `tools.roiextractors` submodule. [PR #117](https://github.com/catalystneuro/neuroconv/pull/117)
+* Added compression and iteration (with options control) to all Fluorescence traces in `write_segmentation`. [PR #120](https://github.com/catalystneuro/neuroconv/pull/120)
 
 ### Documentation and tutorial enhancements:
 * Unified the documentation of NeuroConv structure in the User Guide readthedocs. [PR #39](https://github.com/catalystneuro/neuroconv/pull/39)
diff --git a/src/neuroconv/datainterfaces/ophys/basesegmentationextractorinterface.py b/src/neuroconv/datainterfaces/ophys/basesegmentationextractorinterface.py
index 9c01334f2..fb7cc9947 100644
--- a/src/neuroconv/datainterfaces/ophys/basesegmentationextractorinterface.py
+++ b/src/neuroconv/datainterfaces/ophys/basesegmentationextractorinterface.py
@@ -67,6 +67,8 @@ def run_conversion(
         stub_test: bool = False,
         stub_frames: int = 100,
         include_roi_centroids: bool = True,
+        iterator_options: Optional[dict] = None,
+        compression_options: Optional[dict] = None,
     ):
         from ...tools.roiextractors import write_segmentation
 
@@ -84,4 +86,6 @@ def run_conversion(
             overwrite=overwrite,
             verbose=self.verbose,
             include_roi_centroids=include_roi_centroids,
+            iterator_options=iterator_options,
+            compression_options=compression_options,
         )
diff --git a/src/neuroconv/datainterfaces/ophys/requirements.txt b/src/neuroconv/datainterfaces/ophys/requirements.txt
index f60576484..0d13afeb2 100644
--- a/src/neuroconv/datainterfaces/ophys/requirements.txt
+++ b/src/neuroconv/datainterfaces/ophys/requirements.txt
@@ -1 +1 @@
-roiextractors @ git+https://github.com/catalystneuro/roiextractors.git@9e955248d2532c74522cf896b7c27367304041d9
+roiextractors @ git+https://github.com/catalystneuro/roiextractors.git@e668d4bb3f378a308abc128fa3f4a000f0109ef1
diff --git a/src/neuroconv/tools/roiextractors/roiextractors.py b/src/neuroconv/tools/roiextractors/roiextractors.py
index dbd03fa2f..789915e85 100644
--- a/src/neuroconv/tools/roiextractors/roiextractors.py
+++ b/src/neuroconv/tools/roiextractors/roiextractors.py
@@ -28,6 +28,7 @@
 from hdmf.backends.hdf5.h5_utils import H5DataIO
 
 from .imagingextractordatachunkiterator import ImagingExtractorDataChunkIterator
+from ..hdmf import SliceableDataChunkIterator
 from ..nwb_helpers import get_default_nwbfile_metadata, make_or_load_nwbfile, get_module
 from ...utils import OptionalFilePathType, dict_deep_update, calculate_regular_series_rate
 
@@ -581,10 +582,8 @@ def add_plane_segmentation(
     NWBFile
         The nwbfile passed as an input with the plane segmentation added.
     """
-    if iterator_options is None:
-        iterator_options = dict()
-    if compression_options is None:
-        compression_options = dict()
+    iterator_options = iterator_options or dict()
+    compression_options = compression_options or dict(compression="gzip")
 
     def image_mask_iterator():
         for roi_id in segmentation_extractor.get_roi_ids():
@@ -669,6 +668,8 @@ def add_fluorescence_traces(
     nwbfile: NWBFile,
     metadata: Optional[dict],
     plane_index: int = 0,
+    iterator_options: Optional[dict] = None,
+    compression_options: Optional[dict] = None,
 ) -> NWBFile:
     """
     Adds the fluorescence traces specified by the metadata to the nwb file.
@@ -691,6 +692,8 @@ def add_fluorescence_traces(
     NWBFile
         The nwbfile passed as an input with the fluorescence traces added.
     """
+    iterator_options = iterator_options or dict()
+    compression_options = compression_options or dict(compression="gzip")
 
     # Set the defaults and required infrastructure
     metadata_copy = deepcopy(metadata)
@@ -776,7 +779,7 @@ def add_fluorescence_traces(
 
         # Build the roi response series
         roi_response_series_kwargs.update(
-            data=np.array(trace).T,
+            data=H5DataIO(SliceableDataChunkIterator(trace, **iterator_options), **compression_options),
             rois=roi_table_region,
             **trace_metadata,
         )
@@ -884,6 +887,8 @@ def write_segmentation(
     buffer_size: int = 10,
     plane_num: int = 0,
     include_roi_centroids: bool = True,
+    iterator_options: Optional[dict] = None,
+    compression_options: Optional[dict] = None,
 ):
     """
     Primary method for writing an SegmentationExtractor object to an NWBFile.
@@ -924,6 +929,9 @@ def write_segmentation(
         nwbfile_path is None or nwbfile is None
     ), "Either pass a nwbfile_path location, or nwbfile object, but not both!"
 
+    iterator_options = iterator_options or dict()
+    compression_options = compression_options or dict(compression="gzip")
+
     # parse metadata correctly considering the MultiSegmentationExtractor function:
     if isinstance(segmentation_extractor, MultiSegmentationExtractor):
         segmentation_extractors = segmentation_extractor.segmentations
@@ -978,11 +986,8 @@ def write_segmentation(
                 nwbfile=nwbfile_out,
                 metadata=metadata,
                 include_roi_centroids=include_roi_centroids,
-                iterator_options=dict(buffer_size=buffer_size),
-                compression_options=dict(
-                    compression=True,
-                    compression_opts=9,
-                ),
+                iterator_options=iterator_options,
+                compression_options=compression_options,
             )
 
             # Add fluorescence traces:
@@ -990,6 +995,8 @@ def write_segmentation(
                 segmentation_extractor=segmentation_extractor,
                 nwbfile=nwbfile_out,
                 metadata=metadata,
+                iterator_options=iterator_options,
+                compression_options=compression_options,
             )
 
             # Adding summary images (mean and correlation)
diff --git a/tests/test_ophys/test_tools_roiextractors.py b/tests/test_ophys/test_tools_roiextractors.py
index 35e0afa6b..59614fc02 100644
--- a/tests/test_ophys/test_tools_roiextractors.py
+++ b/tests/test_ophys/test_tools_roiextractors.py
@@ -10,7 +10,7 @@
 from hdmf.testing import TestCase
 from numpy.testing import assert_array_equal, assert_raises
 from parameterized import parameterized, param
-from pynwb import NWBFile, NWBHDF5IO
+from pynwb import NWBFile, NWBHDF5IO, H5DataIO
 from pynwb.device import Device
 from roiextractors.testing import (
     generate_dummy_imaging_extractor,
@@ -616,9 +616,18 @@ def test_add_fluorescence_traces(self):
 
         traces = self.segmentation_extractor.get_traces_dict()
 
-        assert_array_equal(fluorescence["RoiResponseSeries"].data, traces["raw"].T)
-        assert_array_equal(fluorescence["Deconvolved"].data, traces["deconvolved"].T)
-        assert_array_equal(fluorescence["Neuropil"].data, traces["neuropil"].T)
+        for nwb_series_name, roiextractors_name in zip(
+            ["RoiResponseSeries", "Deconvolved", "Neuropil"], ["raw", "deconvolved", "neuropil"]
+        ):
+            series_outer_data = fluorescence[nwb_series_name].data
+            assert_array_equal(series_outer_data.data.data, traces[roiextractors_name])
+
+            # Check compression options are set
+            assert isinstance(series_outer_data, H5DataIO)
+
+            compression_parameters = series_outer_data.get_io_params()
+            assert compression_parameters["compression"] == "gzip"
+
         # Check that df/F trace data is not being written to the Fluorescence container
         df_over_f = ophys.get(self.df_over_f_name)
         assert_raises(
@@ -670,7 +679,14 @@ def test_add_df_over_f_trace(self):
 
         traces = segmentation_extractor.get_traces_dict()
 
-        assert_array_equal(df_over_f[trace_name].data, traces["dff"].T)
+        series_outer_data = df_over_f[trace_name].data
+        assert_array_equal(series_outer_data.data.data, traces["dff"])
+
+        # Check compression options are set
+        assert isinstance(series_outer_data, H5DataIO)
+
+        compression_parameters = series_outer_data.get_io_params()
+        assert compression_parameters["compression"] == "gzip"
 
     def test_add_fluorescence_one_of_the_traces_is_none(self):
         """Test that roi response series with None values are not added to the