diff --git a/.github/workflows/test_and_deploy.yml b/.github/workflows/test_and_deploy.yml
index 8d4ffbba..5d9e017a 100644
--- a/.github/workflows/test_and_deploy.yml
+++ b/.github/workflows/test_and_deploy.yml
@@ -36,16 +36,16 @@ jobs:
       fail-fast: false
       matrix:
         # Run all supported Python versions on linux
-        python-version: ["3.9", "3.10", "3.11"]
         os: [ubuntu-latest]
-        # Include one windows and macos run each of Intel/ARM
+        python-version: ["3.10", "3.11", "3.12"]
+        # Include one windows and two macOS (intel based and arm based) runs
         include:
-        - os: macos-13
-          python-version: "3.11"
-        - os: macos-latest
-          python-version: "3.11"
-        - os: windows-latest
-          python-version: "3.11"
+          - os: macos-13
+            python-version: "3.12"
+          - os: macos-latest
+            python-version: "3.12"
+          - os: windows-latest
+            python-version: "3.12"
 
     steps:
       - name: Cache brainglobe directory
diff --git a/pyproject.toml b/pyproject.toml
index 7fe8417b..0702c7a6 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -5,7 +5,7 @@ authors = [
 ]
 description = "Visualisation and exploration of brain atlases and other anatomical data"
 readme = "README.md"
-requires-python = ">=3.9.0"
+requires-python = ">=3.10"
 dynamic = ["version"]
 
 dependencies = [
@@ -34,9 +34,9 @@ classifiers = [
     "Development Status :: 3 - Alpha",
     "Programming Language :: Python",
     "Programming Language :: Python :: 3",
-    "Programming Language :: Python :: 3.9",
     "Programming Language :: Python :: 3.10",
     "Programming Language :: Python :: 3.11",
+    "Programming Language :: Python :: 3.12",
     "Operating System :: OS Independent",
     "License :: OSI Approved :: BSD License",
     "Intended Audience :: Developers",
@@ -86,7 +86,7 @@ markers = [
 ]
 
 [tool.black]
-target-version = ['py39', 'py310', 'py311']
+target-version = ['py310','py311', 'py312']
 skip-string-normalization = false
 line-length = 79
 
@@ -116,14 +116,14 @@ select = ["I", "E", "F"]
 [tool.tox]
 legacy_tox_ini = """
 [tox]
-envlist = py{39,310,311}
+envlist = py{310,311,312}
 isolated_build = True
 
 [gh-actions]
 python =
-    3.9: py39
     3.10: py310
     3.11: py311
+    3.12: py312
 
 [testenv]
 extras =