diff --git a/README.md b/README.md index 4609bf5..463a156 100644 --- a/README.md +++ b/README.md @@ -28,8 +28,8 @@ conda install -c conda-forge -c bioconda biopython pandas seqtk ## Usage ``` -usage: extract_genes_abricate.py [-h] [-a ABRICATE FILE] [-g GENOMES DIR] - [-o OUTPUT DIR] [-s SUFFIX] +usage: extract_genes_abricate.py [-h] -a ABRICATE FILE -g GENOMES DIR -o OUTPUT DIR [-s SUFFIX] [--genecluster] [--csv] [--flanking] + [--flanking-bp FLANKING LENGTH] [-v] Extract genes from genes based on ABRicate output. @@ -43,6 +43,12 @@ optional arguments: directory for output -s SUFFIX, --suffix SUFFIX Genome assembly file suffix (default: .fasta) + --genecluster Extract all genes to a single fasta if located on a single contig (default: false) + --csv Use this option if your ABRicate output file is comma-separated (default: parse as tab-separated file). + --flanking Extract flanking sequences + --flanking-bp FLANKING LENGTH + Length of flanking sequence to extract in bp (default: 100) + -v, --verbose Increase verbosity ``` **IMPORTANT ASSUMPTIONS**