diff --git a/sample-apps/python-manual-instrumentation-sample-app/requirements.txt b/sample-apps/python-manual-instrumentation-sample-app/requirements.txt
index 74ad8a9b..65d85027 100644
--- a/sample-apps/python-manual-instrumentation-sample-app/requirements.txt
+++ b/sample-apps/python-manual-instrumentation-sample-app/requirements.txt
@@ -1,13 +1,13 @@
-boto3==1.34.45
+boto3==1.35.45
 Flask==2.3.3
-opentelemetry-distro==0.43b0
-opentelemetry-exporter-otlp==1.22.0
-opentelemetry-api==1.22.0
-opentelemetry-instrumentation-flask==0.43b0
-opentelemetry-instrumentation-requests==0.43b0
-opentelemetry-instrumentation-botocore==0.43b0
-opentelemetry-sdk-extension-aws==2.0.1
-opentelemetry-propagator-aws-xray==1.0.1
-protobuf==4.25.3
-PyYAML==6.0.1
-requests==2.32.2
\ No newline at end of file
+opentelemetry-distro==0.48b0
+opentelemetry-exporter-otlp==1.27.0
+opentelemetry-api==1.27.0
+opentelemetry-instrumentation-flask==0.48b0
+opentelemetry-instrumentation-requests==0.48b0
+opentelemetry-instrumentation-botocore==0.48b0
+opentelemetry-sdk-extension-aws==2.0.2
+opentelemetry-propagator-aws-xray==1.0.2
+protobuf==5.28.2
+PyYAML==6.0.2
+requests==2.32.3
\ No newline at end of file