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could transrate be used to merge assembly from trinity #1

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pandafish123 opened this issue Apr 9, 2020 · 0 comments
Open

could transrate be used to merge assembly from trinity #1

pandafish123 opened this issue Apr 9, 2020 · 0 comments

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@pandafish123
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Hi,
I am using Trinity to assemble transcriptomes from 21 samples. Since I cannot assemble the reads from all sample into a single Trinity run which consumes LOTS of RAM, I am assembling each data set independently.
I want to merge all assemblies (21 trinity.fasta files) into a single, unified assembly. I see that transrate has the function that merge-assemblies= ( Merge best contigs from multiple assemblies into file).
So i was wondering whether Transate could be used to merge 21 assemblies into one assembly for DE analysis and so on.

Hope for your advice.

Thanks a lot.

Repick.

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