diff --git a/ci/scripts/matlab_build.sh b/ci/scripts/matlab_build.sh
index 4acd4d3b82a50..235002da3afc6 100755
--- a/ci/scripts/matlab_build.sh
+++ b/ci/scripts/matlab_build.sh
@@ -29,7 +29,6 @@ cmake \
   -S ${source_dir} \
   -B ${build_dir} \
   -G Ninja \
-  -D MATLAB_ARROW_INTERFACE=ON \
   -D MATLAB_BUILD_TESTS=ON \
   -D CMAKE_INSTALL_PREFIX=${install_dir} \
   -D MATLAB_ADD_INSTALL_DIR_TO_SEARCH_PATH=OFF
diff --git a/matlab/CMakeLists.txt b/matlab/CMakeLists.txt
index c7be74de8a951..5380e4cbe1b60 100644
--- a/matlab/CMakeLists.txt
+++ b/matlab/CMakeLists.txt
@@ -379,9 +379,8 @@ endif()
 # Create a subdirectory at CMAKE_INSTALL_PREFIX to install the interface.
 set(CMAKE_INSTALL_DIR "${CMAKE_INSTALL_PREFIX}/arrow_matlab")
 
-if(MATLAB_ARROW_INTERFACE)
-  include(BuildMatlabArrowInterface)
-endif()
+# Build the MATLAB Interface to Arrow.
+include(BuildMatlabArrowInterface)
 
 # Install MATLAB source files.
 # On macOS, exclude '.DS_Store' files in the source tree from installation.
diff --git a/matlab/README.md b/matlab/README.md
index 13b27274d8b7d..1e8565ece4e8c 100644
--- a/matlab/README.md
+++ b/matlab/README.md
@@ -76,12 +76,10 @@ $ cd arrow/matlab
 To build the MATLAB interface, use [CMake](https://cmake.org/cmake/help/latest/):
 
 ```console
-$ cmake -S . -B build -D MATLAB_ARROW_INTERFACE=ON
+$ cmake -S . -B build
 $ cmake --build build --config Release
 ```
 
-**NOTE:** To build the experimental MATLAB interface code, `-D MATLAB_ARROW_INTERFACE=ON` must be specified as shown above.
-
 ## Install
 
 To install the MATLAB interface to the default software installation location for the target machine (e.g. `/usr/local` on Linux or `C:\Program Files` on Windows), pass the `--target install` flag to CMake.
@@ -111,7 +109,7 @@ To run the MATLAB tests, start MATLAB in the `arrow/matlab` directory and call t
 To enable the C++ tests, set the `MATLAB_BUILD_TESTS` flag to `ON` at build time: 
 
 ```console
-$ cmake -S . -B build -D MATLAB_ARROW_INTERFACE=ON -D MATLAB_BUILD_TESTS=ON
+$ cmake -S . -B build -D MATLAB_BUILD_TESTS=ON
 $ cmake --build build --config Release
 ```