diff --git a/packages/jui/src/Tree/SpeedSearchTree/SpeedSearchTree.cy.tsx b/packages/jui/src/Tree/SpeedSearchTree/SpeedSearchTree.cy.tsx
index da1e272a..d911a7e9 100644
--- a/packages/jui/src/Tree/SpeedSearchTree/SpeedSearchTree.cy.tsx
+++ b/packages/jui/src/Tree/SpeedSearchTree/SpeedSearchTree.cy.tsx
@@ -9,6 +9,41 @@ const { Dynamic, HighlightsWithSpace } = composeStories(stories);
 const OS_NORMALIZED_META = Cypress.platform === "darwin" ? "Meta" : "Control";
 
 describe("SpeedSearchTree", () => {
+  it("expands/collapses and navigates nodes by arrow keys", () => {
+    cy.mount(
+      <SpeedSearchTree selectionMode="multiple">
+        <Item title="node 1">
+          <Item>node 1.1</Item>
+          <Item>node 1.2</Item>
+        </Item>
+      </SpeedSearchTree>
+    );
+
+    cy.findByRole("treeitem") // only one should be visible initially
+      .click() // focus
+      .realPress("ArrowRight");
+    // node 1 should be expanded
+    cy.findByRole("treeitem", { name: "node 1.1" });
+    cy.findByRole("treeitem", { name: "node 1.2" });
+    // but selection doesn't go to the children right away
+    cy.findByRole("treeitem", { name: "node 1", selected: true });
+    cy.realPress("ArrowDown");
+    cy.findByRole("treeitem", { name: "node 1.1", selected: true });
+    cy.realPress("ArrowDown");
+    cy.findByRole("treeitem", { name: "node 1.2", selected: true });
+    cy.realPress("ArrowLeft"); // selection should move to node 1 but it remains expanded
+    cy.findByRole("treeitem", { name: "node 1", selected: true });
+    cy.realPress("ArrowLeft"); // the second ArrowLeft closes the node
+    cy.findAllByRole("treeitem").should("have.length", 1);
+
+    cy.realPress("ArrowRight"); // expanding the node again and going down with ArrowRight this time
+    cy.findByRole("treeitem", { name: "node 1", selected: true });
+    cy.realPress("ArrowRight");
+    cy.findByRole("treeitem", { name: "node 1.1", selected: true });
+    cy.realPress("ArrowRight");
+    cy.findByRole("treeitem", { name: "node 1.2", selected: true });
+  });
+
   it("supports Speed Search in dynamic items mode", () => {
     cy.mount(<Dynamic />);
 
diff --git a/packages/jui/src/Tree/Tree.cy.tsx b/packages/jui/src/Tree/Tree.cy.tsx
index 238f6706..1e414ec8 100644
--- a/packages/jui/src/Tree/Tree.cy.tsx
+++ b/packages/jui/src/Tree/Tree.cy.tsx
@@ -8,6 +8,41 @@ import { Item } from "../Collections";
 const { Static, ScrollAndContainerWidth } = composeStories(stories);
 
 describe("Tree", () => {
+  it("expands/collapses and navigates nodes by arrow keys", () => {
+    cy.mount(
+      <Tree selectionMode="multiple">
+        <Item title="node 1">
+          <Item>node 1.1</Item>
+          <Item>node 1.2</Item>
+        </Item>
+      </Tree>
+    );
+
+    cy.findByRole("treeitem") // only one should be visible initially
+      .click() // focus
+      .realPress("ArrowRight");
+    // node 1 should be expanded
+    cy.findByRole("treeitem", { name: "node 1.1" });
+    cy.findByRole("treeitem", { name: "node 1.2" });
+    // but selection doesn't go to the children right away
+    cy.findByRole("treeitem", { name: "node 1", selected: true });
+    cy.realPress("ArrowDown");
+    cy.findByRole("treeitem", { name: "node 1.1", selected: true });
+    cy.realPress("ArrowDown");
+    cy.findByRole("treeitem", { name: "node 1.2", selected: true });
+    cy.realPress("ArrowLeft"); // selection should move to node 1 but it remains expanded
+    cy.findByRole("treeitem", { name: "node 1", selected: true });
+    cy.realPress("ArrowLeft"); // the second ArrowLeft closes the node
+    cy.findAllByRole("treeitem").should("have.length", 1);
+
+    cy.realPress("ArrowRight"); // expanding the node again and going down with ArrowRight this time
+    cy.findByRole("treeitem", { name: "node 1", selected: true });
+    cy.realPress("ArrowRight");
+    cy.findByRole("treeitem", { name: "node 1.1", selected: true });
+    cy.realPress("ArrowRight");
+    cy.findByRole("treeitem", { name: "node 1.2", selected: true });
+  });
+
   it("opens nested expandable single-child items", () => {
     cy.mount(<Static />);
 
diff --git a/packages/jui/src/Tree/TreeKeyboardDelegate.tsx b/packages/jui/src/Tree/TreeKeyboardDelegate.tsx
index fed961d9..c6baa8a3 100644
--- a/packages/jui/src/Tree/TreeKeyboardDelegate.tsx
+++ b/packages/jui/src/Tree/TreeKeyboardDelegate.tsx
@@ -5,11 +5,19 @@ import React, { Key, RefObject } from "react";
 export class TreeKeyboardDelegate<T> extends ListKeyboardDelegate<T> {
   constructor(
     private collection: Collection<Node<T>>,
-    private disabledKeys: Set<Key>,
+    disabledKeys: Set<Key>,
     ref: RefObject<HTMLElement>,
     collator?: Intl.Collator
   ) {
-    super(collection, disabledKeys, ref, collator);
+    super({
+      collection,
+      disabledKeys,
+      ref,
+      collator,
+      // Since @react-aria/selection@3.16.0 getKeyLeftOf and getKeyRightOf is
+      // removed if orientation is vertical and layout is "stack".
+      layout: "grid",
+    });
   }
 
   getKeyLeftOf(key: React.Key): React.Key {