Hifi and ONT behave differently in 3D-DNA(same species)

[anxuan-web]

We assembled the genome if the same species using Hifi and ONT data, respectively, and both using Hic data to assist assembly.
We find that there was some big rearrangement between the two genomes. For example, about 20M of the sequence in one chromosome is in a different location.

How do we choose? Which result is correct

[anxuan-web]

Blue represents the genome of the HIFI data assembly and green represents the ONT data.

[dudcha]

Use Juicebox assembly tools. You can find useful instructions on how to interpret contact maps in the Genome assembly cookbook hosted on dnazoo.org/methods. Best, -OlgaOn Jul 22, 2023, at 3:37 AM, anxuan-web ***@***.***> wrote: Blue represents the genome of the HIFI data assembly and green represents the ONT data. —Reply to this email directly, view it on GitHub, or unsubscribe.You are receiving this because you are subscribed to this thread.Message ID: ***@***.***>