diff --git a/.github/workflows/test-and-zip-default-themes.yml b/.github/workflows/test-and-zip-default-themes.yml
index ed48ad2868ec1..18dd365a71af3 100644
--- a/.github/workflows/test-and-zip-default-themes.yml
+++ b/.github/workflows/test-and-zip-default-themes.yml
@@ -164,6 +164,7 @@ jobs:
       fail-fast: false
       matrix:
         theme: [
+            'twentytwentyfive',
             'twentytwentyfour',
             'twentytwentythree',
             'twentytwentytwo',
diff --git a/tests/performance/specs/home.test.js b/tests/performance/specs/home.test.js
index b88b6adb9f362..004c8f03debd4 100644
--- a/tests/performance/specs/home.test.js
+++ b/tests/performance/specs/home.test.js
@@ -14,7 +14,7 @@ const results = {
 	lcpMinusTtfb: [],
 };
 
-const themes = [ 'twentytwentyone', 'twentytwentythree', 'twentytwentyfour' ];
+const themes = [ 'twentytwentyone', 'twentytwentythree', 'twentytwentyfour', 'twentytwentyfive' ];
 
 const locales = [ 'en_US', 'de_DE' ];
 
diff --git a/tests/phpunit/tests/theme.php b/tests/phpunit/tests/theme.php
index 5f9ef3959ead1..48576672b9a26 100644
--- a/tests/phpunit/tests/theme.php
+++ b/tests/phpunit/tests/theme.php
@@ -23,6 +23,7 @@ class Tests_Theme extends WP_UnitTestCase {
 		'twentytwentytwo',
 		'twentytwentythree',
 		'twentytwentyfour',
+		'twentytwentyfive',
 	);
 
 	/**