v1.7.0

New Features:

• Support for the output of unmerged paired end files. This allows users to use Dada2 for sequence variant calling.
• The API is now documented at ReadTheDocs

v1.6.4

Fix for issue validating fastq.gz files that was not solved by v1.6.3

v1.6.3

Fixed issue validating fastq.gz and added tests.

v1.6.2

This release fixes an error that occasionally occurred when validating FASTQ files. ITSxpress used BBtools reformat.sh which occasionally threw an exception when validating FASTQ files due to a race condition. FASTQ file validation is now done with Biopython instead.

v1.6.1

• Changed the default clustering identity to 99.5%.
• Experiments with fungal soil samples showed that ITSxpress and ITSx trimmed 99.822% of reads in the ITS1 region within 2 bases of each other and 99.099% of reads in the ITS2 region within 2 bases of each other at 99.5% identity. For higher accuracy, dereplication can be run at at 100% identity.

v1.6.0

This release adds a new feature to cluster merged sequences at less than 100% identity. This speeds up typical dataset trimming by about 10x over previous versions depending on the sample, without major effects on trimming accuracy. This feature is controlled with the --cluster_id flag. Default behavior is now to cluster at 0.987 identity.

v1.5.7

Improved documentation

v1.5.6

• Database is now included in the release

v1.5.5

Fixed documentation issues for Pip

v1.5.4

• Fixed bug in handling of temporary files files specified with the --tempfile flag
• updated readme
• fixed issues with exception handling